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Application of sea cucumber enzymatic hydrolysate in preparation of medicine for improving phlebothrombosis

A technology of venous thrombosis and enzymatic hydrolysis solution, which is applied in the field of medicine, can solve the problems of biological activity destruction of active substances, achieve the effects of improving venous thrombosis, protecting the fibrinolytic system, and protecting from damage

Inactive Publication Date: 2018-09-25
GUANGDONG OCEAN UNIVERSITY +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the biological activity of the single extract of sea cucumber is damaged to a certain extent during the extraction process, so it is necessary to make full use of sea cucumber while ensuring its biological activity.

Method used

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  • Application of sea cucumber enzymatic hydrolysate in preparation of medicine for improving phlebothrombosis
  • Application of sea cucumber enzymatic hydrolysate in preparation of medicine for improving phlebothrombosis
  • Application of sea cucumber enzymatic hydrolysate in preparation of medicine for improving phlebothrombosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] This example is an example of thrombolysis in vivo.

[0046] (1) Experimental grouping and processing

[0047] 84 mice were randomly divided into 5 groups after routine feeding for one week: blank control group (n=16), model control group (n=20), high-dose group (n=16), middle-dose group (n=16) , low dose group (n=16). All groups were administered by intragastric administration, and administered at a dosage of 0.1mL / 10g.bw. Feed for 30 days with weighing every 5 days. The grouping and processing methods are shown in Table 1.

[0048] Table 1 Grouping and processing methods

[0049]

[0050] (2) Preparation of mouse venous thrombosis model

[0051] Modeling was started on the 30th day, and the carrageenan was accurately weighed, and the mass concentration of 0.2% was prepared with physiological saline; after the mice were weighed, the mice were administered by intraperitoneal injection. The injection dose is 0.1mL / 10g.bw. The blank control group was injected wi...

Embodiment 2

[0069] This example is an example of antithrombotic in vitro.

[0070] (1) Antithrombotic model experiment in vitro

[0071] Take fresh New Zealand white rabbit blood, coagulate into clots in the natural state, and divide the blood clots into five equal parts, 0.5g each, and put them in centrifuge tubes. The high-dose group is 800mg / kg, the middle-dose group is 400mg / kg, and the low-dose group is 200mg / kg. Do 6 parallels for each set. Then 1 mL of the corresponding solution was added to the centrifuge tube, and incubated on a constant temperature shaker at 37° C. for 5 h. Finally, take out the remaining plug and weigh it quickly.

[0072] Thrombolysis rate = (weight before thrombolysis - weight after thrombolysis) / weight before thrombolysis × 100%

[0073] (2) Fibrin plate method to measure the in vitro fibrinolytic activity of sea cucumber enzymatic hydrolyzate

[0074] Solution preparation:

[0075] ② Accurately weigh 0.075g of fibrinogen into a conical flask filled ...

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PUM

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Abstract

The invention discloses application of sea cucumber enzymatic hydrolysate in preparation of a medicine for improving phlebothrombosis. The sea cucumber enzymatic hydrolysate has better in-vivo thrombolysis effect; the sea cucumber enzymatic hydrolysate has the advantages that on the one hand, thrombus can be directly improved by activating a fibrinolytic system, on the other hand, by anti-inflammatory effect, the vascular endothelial cell can be prevented from being injured, and the fibrinolytic system is indirectly protected, and certain improving effect on the phlebothrombosis is finally realized. In addition, the sea cucumber enzymatic hydrolysate can also be used for thrombolysis in vitro.

Description

technical field [0001] The invention relates to the technical field of medicine, and more specifically relates to the application of sea cucumber enzymatic hydrolyzate in the preparation of medicine for improving venous thrombosis. Background technique [0002] Sea cucumber is a health-care functional food. After the sea cucumber is caught, remove the viscera, wash the sediment in the cavity, boil it in appropriate salt water for about 1 hour, pick it up and let it cool, and expose it to the sun or bake it until it is 80% to 90% dry. When it is hot, put it into the liquid of the leaves and cook for a while, until the color turns black, take it out and dry it in the sun. [0003] Sea cucumbers are rich in more than 50 natural and precious active substances such as protein, minerals, and vitamins. Among them, acidic mucopolysaccharides and chondroitin can significantly reduce the amount of lipofuscin in heart tissue and skin proline, and play a role in delaying aging. The 18 ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/616A61P7/02A61P29/00
CPCA61K35/616A61P7/02A61P29/00
Inventor 宋采杨隆恩张永平张才张翼秦邦伟卢虹玉
Owner GUANGDONG OCEAN UNIVERSITY
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