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Dioscin preparation method

A technology of diosgenin and diosgenin, which is applied in the field of diosgenin preparation, can solve the problems of unstable product quality, large amount of acid, and long time consumption, and achieve high content of active ingredients in the solution, strong oxidation resistance, and avoid heating. Effect

Inactive Publication Date: 2018-09-25
NANJING ZELANG AGRI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the large amount of acid used in the acid hydrolysis method, there are many by-products of acid degradation organic matter, resulting in large sewage discharge and serious environmental pollution, which limits the industrialization of diosgenin.
The pre-fermentation method is used to extract diosgenin, but this method is time-consuming, and the product quality is not stable enough, often due to long-term natural fermentation to produce smilagenin ketone and epismilagenin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Prepare Dioscorea scutellaria, pulverize it, grind it into powder, add citric acid-disodium hydrogen phosphate buffer solution with a pH value of 4.5 at a material-to-liquid ratio of 1:5, add 15% cellulase by mass after uniformity, and heat at 55°C After enzymatic hydrolysis for 1 h, centrifuge at 4000 r / min for 10 min, collect the supernatant, take an appropriate amount of enzymatically broken wall sediment, and add warm water (30°C), 50% absolute ethanol, 0.05 mol / L citric acid-sodium hydrogen phosphate buffer solution with a pH value of 3.7, extracted for 30 min, and placed the material in a 30KHz ultrasonic device for extraction under the same conditions. After the extraction, centrifuged at 4000 r / min for 10 min, and took the The supernatants were combined and dried at 60°C to obtain diosgenin, with an extraction rate of 85.28%. According to UV detection, the content of diosgenin was 83.5%.

Embodiment 2

[0022] Prepare Dioscorea scutellaria, pulverize it, grind it into powder, add citric acid-disodium hydrogen phosphate buffer solution with a pH value of 4.5 at a material-to-liquid ratio of 1:5, add 15% cellulase by mass after uniformity, and heat at 55°C After enzymatic hydrolysis for 1 h, centrifuge at 4000 r / min for 10 min, collect the supernatant, take an appropriate amount of enzyme-broken sediment, add warm water (45°C), 55% absolute ethanol, 0.05 mol / L citric acid-disodium hydrogen phosphate buffer solution with a pH value of 3.7, extract for 35 min, and extract the material in a 30KHz ultrasonic device under the same conditions. After the extraction, centrifuge at 4000 r / min for 10 min, and take the The supernatants were combined twice and dried at 60°C to obtain diosgenin, with an extraction rate of 87.35%. According to UV detection, the content of dioscin was 85.8%.

Embodiment 3

[0024] Prepare Dioscorea scutellaria, pulverize it, grind it into powder, add citric acid-disodium hydrogen phosphate buffer solution with a pH value of 4.5 at a material-to-liquid ratio of 1:5, add 15% cellulase by mass after uniformity, and heat at 55°C After enzymatic hydrolysis for 1 h, centrifuge at 4000 r / min for 10 min, collect the supernatant, take an appropriate amount of enzyme-broken wall sediment, add warm water (50°C), 60% absolute ethanol, 0.05 mol / L citric acid-disodium hydrogen phosphate buffer solution with a pH value of 3.7 was extracted for 40 min, and the material was extracted in a 30KHz ultrasonic device under the same conditions. After the extraction was completed, centrifuged at 4000 r / min for 10 min, and the The supernatants were combined twice and dried at 60°C to obtain diosgenin, with an extraction rate of 88.21%. According to UV detection, the content of dioscin was 90.2%.

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Abstract

The invention relates to a dioscin preparation method, particularly to a method for extracting dioscin by using an enzyme method-ultrasound wall breaking method. The treatment steps comprise: preparing Dioscorea zingiberensis, crushing, grinding to obtain powder, adding to a citric acid-disodium hydrogen phosphate buffer solution having a pH value of 4.5 according to a material-to-liquid ratio of1:5, uniformly mixing, adding 15% by mass of cellulase, carrying out enzymolysis for 1 h at a temperature of 55 DEG C, carrying out centrifugation for 10 min at a rate of 4000 r / min, and collecting the supernatant; taking an appropriate amount of the enzyme method wall breaking precipitate, respectively adding warm water, 50-60% absolute ethanol and 0.05 mol / L citric acid / disodium hydrogen phosphate buffer solution having a pH value of 3.7 according to a material-to-liquid ratio of 1:5, extracting for 30-40 min, carrying out ultrasonic wave assisted extraction under the same conditions, carrying out centrifugation for 10 min at a rate of 4000 r / min after completing the extraction, and taking the supernatant; and combining the two supernatants, and drying at a temperature of 60 DEG C to obtain the dioscin. According to the present invention, the method is efficient, the effective component of the solution is high, and the anti-oxidation property of the solution is strong.

Description

technical field [0001] The invention relates to a preparation method of diosgenin, which belongs to the field of extracting active ingredients of traditional Chinese medicine. Background technique [0002] Dioscorea saponin, also known as diosgenin, is a typical natural saponin compound, widely found in medicinal plants such as Dioscoreaceae, Liliaceae, Caryophyllaceae and Rosaceae, such as Dioscorea punctatus, Dioscorea shield leaf and Chinese yam Wait. It is not only the main drug source for the production of drugs for the treatment of cardiovascular diseases, but also one of the important drug sources for the production of diosgenin, which can be used to synthesize various contraceptives and steroid hormone drugs. Dioscorea is a plant of the genus Dioscorea in the family Dioscoreaceae. It is a medicinal and edible plant with a long history of medicinal use. In recent years, with the continuous development of science and technology, the research on diosgenin is also chang...

Claims

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Application Information

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IPC IPC(8): C07J71/00
CPCC07J71/0005
Inventor 杨成东
Owner NANJING ZELANG AGRI DEV
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