Preparation method of biodiesel of pyrenoid chlorella by using cassava residue as primary raw material
A technology of chlorella pyrenoidosa and biodiesel, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of high cultivation cost and low efficiency of biodiesel produced by algae
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Embodiment 1
[0025] A method for preparing oil and fat from chlorella pyrenoidosa cells using cassava residue as a main raw material, which comprises the following steps:
[0026] Streak inoculation of Trichoderma reesei on the PDA medium and culture to obtain a single colony; pick a single colony and inoculate it into a primary seed medium for cultivation, and then perform secondary seed medium cultivation to obtain a Trichoderma reesei seed liquid; The first-level seed culture medium and the second-level seed culture medium are both PDA liquid culture medium;
[0027] Pick Chlorella pyrenoidosa, plant it in a container containing a growth medium, cultivate it at 28°C with a light intensity of 6000 lux, shake the container 2-3 times a day, wait until it reaches the logarithmic growth phase, and obtain a Chlorella pyrenoidosa liquid; The components of the growth medium are: glucose 10g / L, ammonium chloride 2g / L, sodium nitrate 1g / L, potassium dihydrogen phosphate 0.5g / L, sodium chloride 0....
Embodiment 2
[0033] A method for preparing oil and fat from chlorella pyrenoidosa cells using cassava residue as a main raw material, which comprises the following steps:
[0034] Streak inoculation of Trichoderma reesei on the PDA medium and culture to obtain a single colony; pick a single colony and inoculate it into a primary seed medium for cultivation, and then perform secondary seed medium cultivation to obtain a Trichoderma reesei seed liquid; The first-level seed culture medium and the second-level seed culture medium are both PDA liquid culture medium;
[0035] Pick Chlorella pyrenoidosa, plant it in a container containing a growth medium, cultivate it at 28°C with a light intensity of 6000 lux, shake the container 2-3 times a day, wait until it reaches the logarithmic growth phase, and obtain a Chlorella pyrenoidosa liquid; The components of the growth medium are: glucose 10g / L, ammonium chloride 2g / L, sodium nitrate 1g / L, potassium dihydrogen phosphate 0.5g / L, sodium chloride 0....
Embodiment 3
[0047] The dry weight content of biomass, the total fat content (percentage of dry weight of biomass) and oil production were detected in the examples and comparative examples of the present invention. The specific test results are shown in Table 1;
[0048] Table 1
[0049] group
[0050]Conclusion: the impact of strain type, timing of addition on biomass dry weight content, total fat content and oil production was verified by Table 1, and it was found that the dry weight content, total fat content and oil production indicators of Example 1 were higher than those of the comparison Ratio 1-3; Comparative Example 1 does not add bacterial strains, only adopts algae culture, all aspects of indicators are the lowest, oil production is about 40% lower than that of Example 1; Comparative Example 2 only uses Trichoderma reesei, wherein Trichoderma reesei Utilize fermented cassava residue to produce reducing sugar, reducing sugar can promote the growth rate of algae, increa...
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