Bacillus amyloliquefaciens for preventing and curing strawberry wilt and application thereof

A technology for dissolving amyloid spores and bacilli, applied in the application, bacteria, fungicides and other directions, can solve the problems of poor control efficiency and insufficient colonization ability of antagonistic bacteria, so as to reduce the occurrence, production and transportation costs of strawberry fusarium wilt Low, easy to process effect

Active Publication Date: 2018-09-28
SHANDONG INST OF POMOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the deficiencies of the prior art, the present invention provides a Bacillus amyloliquefaciens JM-3 for preventing and treating strawberry wilt and its application, which solves the problems of insufficient colonization ability of antagonistic bacteria and poor control efficiency in the existing biocontrol technology

Method used

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  • Bacillus amyloliquefaciens for preventing and curing strawberry wilt and application thereof
  • Bacillus amyloliquefaciens for preventing and curing strawberry wilt and application thereof
  • Bacillus amyloliquefaciens for preventing and curing strawberry wilt and application thereof

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Experimental program
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Effect test

Embodiment 1

[0025] The present invention isolates multiple probiotic antagonistic bacterial strains from the soil with a better effect of solving continuous cropping obstacles, and screens and obtains a bacterial strain with high antagonistic performance to Fusarium wilt of strawberry through ultraviolet mutagenesis and stress domestication, named JM-3 , the 16s rDNA sequence of the strain was determined, and a gene sequence fragment with a length of 1413bp was obtained. After sequence comparison, the strain JM-3 was identified as Bacillus amyloliquefaciens.

[0026] The above-mentioned Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) JM-3 was preserved in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures on March 12, 2018. The preservation address is: No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, Chinese Academy of Sciences Microbiology Institute, deposit number: CGMCC No.15444.

[0027] The 16S rDNA nucleotide seq...

Embodiment 2

[0033] According to the routine operation in this field, activate and purify the strain of Fusarium wilt of strawberry, on the ultra-clean workbench, inoculate the pre-activated fungus cake of Fusarium wilt of strawberry with a diameter of 7mm in the center of the PDA solid medium plate, and cultivate in the dark at 25°C After 24h, pick the activated Bacillus amyloliquefaciens JM-3 single bacterium colony described in embodiment 1 with a toothpick, and inoculate 1 point respectively at the upper, lower, left and right 4 positions at the center of the bacterium cake at a distance of 2 cm. Seal with plastic wrap, incubate in the dark at 25°C for 3-4 days, and observe the results.

[0034] The antagonistic effect of Bacillus amyloliquefaciens JM-3 and the target pathogen Fusarium wilt figure 2 , the inhibitory effect of Bacillus amyloliquefaciens JM-3 on Strawberry Wilt was very obvious, and the inhibition zone was clearly visible.

Embodiment 3

[0036] Pick a single colony of activated Bacillus amyloliquefaciens JM-3, inoculate it into a 250mL Erlenmeyer flask filled with about 100mL of sterilized LB liquid medium, and culture it on a shaker at 37°C and 150rpm for 24h to obtain a bacterial cell concentration of 10 8 ~10 9 cfu mL -1 Bacillus amyloliquefaciens JM-3 bacterial fluid.

[0037] Take soil from the field where strawberry wilt takes place seriously, put it in a plastic flowerpot with a diameter of 15cm, transplant strawberry seedlings, and water 20mL of strawberry rhizosphere in the treatment group with a concentration of 10 8 ~10 9 cfu mL -1 Bacillus amyloliquefaciens JM-3 bacterial fluid, the control group replaced the bacterial fluid with water. Each treatment was replicated 3 times, and each replicated 10 pots. With conventional cultivation and management, the incidence of strawberry wilt in each treatment group was investigated every 30 days, and the relative control effect was calculated for 4 conse...

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Abstract

The invention relates to a bacillus amyloliquefaciens for preventing and curing strawberry wilt and application thereof. The bacillus amyloliquefaciens JM-3 is deposited at the general microorganism center of China Committee for Culture Collection of Microorganisms on March 12, 2018, the deposition address is Institute of Microbiology, Chinese Academy of Sciences, No. 3, yard 1, beichen west road,Chaoyang District of Beijing, and the deposition number is CGMCC No.15444. Microbial preparation prepared by fermentation broth of the bacillus amyloliquefaciens JM-3 has a lower moisture content, bacterial cells are in a semi-dormant state, spores have high stress resistance, and the storage period of the preparation can be up to 2 years; and the microbial preparation of the bacillus amyloliquefaciens JM-3 can effectively reduce the occurrence of strawberry wilt and reduce the amount of chemicals used.

Description

technical field [0001] The invention relates to a functional microorganism, more specifically to a Bacillus amyloliquefaciens JM-3 for preventing and treating strawberry wilt and its application, belonging to the technical field of biological pesticides. Background technique [0002] Strawberry wilt is an important soil-borne disease caused by Fusarium oxysporum f.sp. Due to the special cultivation conditions and perennial planting methods of strawberry, the disease is common and increasingly serious in the main strawberry producing areas, and has become one of the main diseases affecting the economic benefits and sustainable development of the strawberry industry. [0003] At present, the control of strawberry wilt in my country is still dominated by chemical agents. The frequent and irrational use of fungicides not only easily leads to the outbreak of drug resistance of pathogenic bacteria, but also poses a serious threat to the ecological environment and human health. Bi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07
CPCA01N63/00C12N1/20C12N1/205C12R2001/07
Inventor 姜莉莉孙瑞红王红艳宫庆涛武海斌刘政军方新军
Owner SHANDONG INST OF POMOLOGY
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