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Method for determining contents of paraquat and diquat in biological fluid

A biological fluid and paraquat technology, which is applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of difficult separation, high detection limit, and difficult popularization and application, and achieve the effects of low cost, high sensitivity and strong anti-interference ability.

Active Publication Date: 2018-09-28
SOUTHERN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Paraquat and diquat have similar structures and are not easy to separate. At the same time, false positive results and false negative results are often caused by the interference of endogenous compounds. The LC-MS method has high selectivity and sensitivity, but often requires hydrophilic interaction. Functional chromatographic columns and instrument consumables are expensive to detect and maintain, making it difficult to popularize and apply them clinically

Method used

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  • Method for determining contents of paraquat and diquat in biological fluid
  • Method for determining contents of paraquat and diquat in biological fluid
  • Method for determining contents of paraquat and diquat in biological fluid

Examples

Experimental program
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Embodiment 1

[0023] Example 1 (measurement method example)

[0024] 1. Instruments, reagents and samples

[0025] 1.1. Instruments: Vortex-Genie adjustable vortex mixer; Shumei KQ-500DB CNC ultrasonic cleaner; micro pipette; MiLLi-Q ELement ultrapure water treatment system; 1 / 1000 electronic balance; G13U desktop centrifuge ; LC-20A high performance liquid chromatography (SPD-M20A diode array detector); Phenomenex Synergi Max-RP 80A-C12 chromatographic column (250 × 4.6mm, 4μm).

[0026] 1.2. Reagents: Triton X-114; NaCl (chromatographically pure); formic acid (chromatographically pure); sodium octane sulfonate (chromatographically pure); phosphoric acid; diethylamine; acetonitrile (chromatographically pure).

[0027] Prepare 0.14g / mL Triton X-114 solution: Take a 100mL volumetric flask, weigh and peel it with a 1 / 1000 electronic balance, add 14g Triton X-114, dilute to the mark with ultrapure water, shake well and put in Refrigerate at -4°C for 4 hours, take it out and return to room te...

Embodiment 2

[0058] Embodiment 2 (kit product example)

[0059] The kit of this embodiment includes reagent A, reagent B, reagent C, reagent D, reagent E and reagent F, and the preparation method of each reagent is as follows.

[0060] Reagent A: 0.14g / mL Triton X-114 solution; the preparation method is as follows, take 14.0g of Triton X-114, add pure water to 100mL;

[0061] Reagent B: 0.1308g / mL NaCl solution; the preparation method is as follows, take 6.54g of NaCl, add pure water and dilute to 50mL;

[0062] Reagent C: 5mL formic acid;

[0063] Reagent D: 0.1% formic acid solution; the preparation method is as follows, take 100 μL of formic acid, add pure water to dilute to 100 mL;

[0064] Reagent E: 5mmol / L sodium octane sulfonate aqueous solution (containing 0.4% phosphoric acid) 1000mL; the preparation method is as follows, weigh 1.081g sodium octane sulfonate into a 1000mL volumetric flask, dissolve with 200mL water, add 4mL phosphoric acid, and then Add diethylamine to adjust ...

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Abstract

The invention relates to a method for testing or analyzing a material by determining the chemical or physical properties of the material, in particular to a method for determining the contents of paraquat and diquat in biological fluid. The method comprises the steps: taking a fluid sample and sequentially adding a Triton X-114 aqueous solution, a NaCl aqueous solution and formic acid, so that thefinal concentration of Triton X-114 is 0.0545 to 0.109g / mL, the final concentration of NaCl is 0.0165 to 0.0495g / mL, and the final concentration of formic acid is 15 to 30 muL / mL; then oscillating, ultrasonically treating in a water bath, centrifuging, taking a supernatant, and filtering to obtain a sample injection solution of a sample to be determined; taking standard solutions of different concentrations of the same volume with the fluid sample to prepare a series of concentration standard product sample injection solutions; detecting the sample injection solution of the sample to be determined and a series of concentration standard product sample injection solutions by a liquid phase ion pair chromatography to obtain liquid phase chromatograms of the sample to be determined and standard products, the chromatograms thereof are compared to determine the characteristic peak, and then the content of paraquat or diquat in the sample is detected by calculation of a calibration curve according to the peak area.

Description

technical field [0001] The invention relates to testing or analyzing materials by means of measuring their chemical or physical properties, in particular to a method for measuring the content of bipyridine compounds in human tissue. Background technique [0002] Both paraquat and diquat belong to bipyridine compounds, which are non-selective contact herbicides and are highly toxic to humans and animals. Currently, there is no effective detoxification method. The mortality rate of patients with paraquat poisoning in my country has As high as 85% to 90%. Studies have shown that paraquat and diquat can enter the human body through the skin, mucous membranes, respiratory tract, digestive tract, etc., and are distributed in the heart, liver, brain, kidney and other organs. Among them, the lungs are the most distributed and the most damaged. Pulmonary fibrosis Accompanied by respiratory failure is the main cause of death in patients with late poisoning. Modern medicine generally ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/14
CPCG01N30/02G01N30/06G01N30/14
Inventor 潘加亮袁嘉豪黄清湄马安德
Owner SOUTHERN MEDICAL UNIVERSITY
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