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A kind of chlorella growth promoter and preparation method thereof

A growth promoter and a technology for Chlorella, applied in the field of Chlorella growth promoter and its preparation, can solve the problem of lack of stable and effective Chlorella genetic transformation and separation system, affecting the growth and metabolic state of algal cells, and reducing the total output of microalgae and other problems, to achieve the effect of improving potential, improving photosynthesis, and easy operation

Active Publication Date: 2022-03-04
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are still some problems to be solved urgently in the above work: (1) Some irradiation treatments and stress treatments will affect the growth and metabolism of algal cells, and even lead to a decrease in the total production of microalgae. Multi-stage culture needs to transfer algal cells, so the operation process is relatively strict consume more training costs
(2) There is a lack of a stable and effective Chlorella genetic transformation isolation system, and the expression of recombinant vectors in the nucleus or chloroplasts is transient and random

Method used

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  • A kind of chlorella growth promoter and preparation method thereof

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Effect test

Embodiment 1

[0030] A chlorella growth promoter of the present embodiment includes the following raw materials in parts by weight: 7 parts of regulator, 0.005 part of quercetin, 0.06 part of nano selenium, 992.935 parts of polyethylene glycol; wherein, the regulator It is prepared by mixing micro-nano bio-silicon powder and pistachio chinensis extract dry powder according to the mass ratio of 2.5:5. Compared with the blank control group, the growth promotion rate of chlorella on the 4th day of the treatment group added with extracts can reach 3.43 times of that of the normal treatment group.

Embodiment 2

[0032] A chlorella growth promoter of the present embodiment includes the following raw materials in parts by weight: 2 parts of regulator, 0.015 part of quercetin, 0.7 part of nano-selenium, and 997.285 parts of polyethylene glycol; wherein, the regulator It is prepared by mixing micro-nano bio-silicon powder and pistachio chinensis extract dry powder according to the mass ratio of 3:5.5. Compared with the blank control group, the growth promotion rate of chlorella on the 4th day of the treatment group added with the extract was 1.93 times that of the normal treatment group.

Embodiment 3

[0034] A chlorella growth promoter of the present embodiment includes the following raw materials in parts by weight: 4 parts of regulator, 0.035 part of quercetin, 0.1 part of nano selenium, 995.865 parts of polyethylene glycol; wherein, the regulator It is prepared by mixing micro-nano bio-silicon powder and pistachio chinensis extract dry powder according to the mass ratio of 2.5:6. Compared with the blank control group, the growth promotion rate of chlorella on the 4th day of the treatment group added with the extract can reach 2.47 times that of the normal treatment group.

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Abstract

The invention discloses a chlorella growth promoter and a preparation method thereof. The chlorella growth promoter comprises the following raw materials in parts by weight: 0.1-10 parts of regulator, 0.001-2 parts of quercetin, nano 0.005-1 part of selenium, 987-999.894 parts of polyethylene glycol; wherein the regulator is formed by mixing micro-nano bio-silicon powder and Pistacia chinensis extract dry powder according to the mass ratio (2-3):(5-7); The invention opens up a new way for industrial cultivation of microalgae growth, with simple materials, convenient operation and low cost; the products used are green, environmentally friendly and safe, and plant-derived flavonoids can be used as a natural plant stimulant to promote photosynthetic metabolism of algae cells The reaction improves the growth potential of chlorella and the accumulation of carbon source substrates in chlorella cells, thereby increasing its growth rate and solving the problem of industrialized cultivation of rapid growth of microalgae.

Description

technical field [0001] The invention belongs to the field of biomass resource utilization and bioenergy, and in particular relates to a chlorella growth promoter and a preparation method thereof. Background technique [0002] Chlorella has the characteristics of stable reproduction speed, fast cultivation system and low demand for cultivated land. It can be industrially cultivated in high-efficiency bioreactors for large-scale production and application. Chlorella biomass production rates have also been reported to exceed those of many terrestrial plants. It has high economic value and can be used to produce biodiesel, protein, and secondary metabolites carotenoids, astaxanthin, especially unsaturated fatty acids, etc., so it is widely used in the development of green and sustainable bioenergy. When exogenous biostimulants are added to the chlorella culture medium, it can cause stress, improve its growth potential, and promote photosynthesis efficiency and biomass. The hig...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/38C12N1/12C12R1/89
CPCC12N1/12C12N1/38
Inventor 吴丽芳马瑜晗
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI