Unlock instant, AI-driven research and patent intelligence for your innovation.

Microbial preparation for low-temperature soil remediation and its preparation method and application

A compound microbial agent and soil remediation technology, applied in the field of soil remediation, can solve problems such as inability to remediate soil, unstable enzyme activity, single treatment effect, etc., to solve environmental pollution problems, simple preparation method, suitable for large-scale production Effect

Active Publication Date: 2020-12-08
安徽中富恒源生物科技有限公司
View PDF10 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Pesticides are the largest and most toxic chemical substances that people actively put into the environment. After pesticides enter the soil, they mainly have two destinations: adsorption and degradation. Adsorption is accomplished by the fixation and complexation of organic matter or clay minerals. , due to changes in the status of pesticides, the ecotoxicity of pesticides can be reduced within a certain range, but this is a passive process. At present, there are many studies on the use of enzymes to remediate soil pesticide pollution, but simple enzyme remediation has great limitations, and simple enzyme activity unstable, prone to deactivation
[0004] According to investigations, the pollution has continued to remote cold areas. There are also many reports on the use of microbial agents for soil remediation. However, because many microorganisms cannot tolerate low temperature environments, or the treatment effect is single, it cannot be fundamentally treated. overall soil restoration
[0005] Soil polyphenol oxidase is mainly derived from enzymes released from the decomposition of soil microorganisms, plant root exudates, and animal and plant residues. It is a compound enzyme that can effectively repair soil, but it also has a single and unstable effect. In order to be able to To more effectively repair the problems of pesticide pollution, organic matter pollution, and ammonia nitrogen pollution of soil in low-temperature areas, it is urgent to find a solution that can comprehensively solve the above pollution problems

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Microbial preparation for low-temperature soil remediation and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Immobilization of spiny root peroxidase: add mesoporous molecular sieve material SBA-15 to spiny root peroxidase with RZ value of 3.2, at a temperature of 5°C; pH of 5.0; rotational speed of 220rpm; immobilization time Obtained after immobilization under 12h.

[0025] Immobilization of soil polyphenol oxidase: add mesoporous molecular sieve material SBA-15 to soil polyphenol oxidase, after immobilization at a temperature of 5°C; pH of 7; rotation speed of 220rpm; immobilization time of 12h get.

[0026] Preparation of the psychrotrophic bacterium Pseudomonas roserubinus HD1031 inoculum: culture the psychrophilic tolerant bacterium Pseudomonas roserubinus HD1031 to the logarithmic phase, and then inoculate the strains cultivated to the logarithmic phase according to the inoculation amount of 2% of the medium volume Into the culture medium, the medium is a medium with wheat straw powder and sweet potato vine powder as carbon sources, ammonium sulfate and peptone as nitro...

Embodiment 2

[0032] Immobilization of spiny root peroxidase: add mesoporous molecular sieve material SBA-15 to spiny root peroxidase with an RZ value of 3.2, at a temperature of 20°C; pH of 5.0; rotational speed of 150rpm; immobilization time of Obtained after immobilization under 24h.

[0033] Immobilization of soil polyphenol oxidase: Add mesoporous molecular sieve material SBA-15 to soil polyphenol oxidase, after immobilization at a temperature of 20°C; pH of 10; rotation speed of 150rpm; immobilization time of 24h get.

[0034] Preparation of the psychrotrophic bacterium Pseudomonas roserubin HD1031 inoculum: culture the psychrogenic tolerant bacterium Pseudomonas roserubinus HD1031 to the logarithmic phase, and then inoculate the strains cultivated to the logarithmic phase according to the inoculation amount of 5% of the medium volume Into the culture medium, the medium is a medium with wheat straw powder and sweet potato vine powder as carbon sources, ammonium sulfate and peptone as...

Embodiment 3

[0040] Immobilization of spiny root peroxidase: add mesoporous molecular sieve material SBA-15 to spiny root peroxidase with RZ value of 3.2, at a temperature of 12°C; pH of 5.0; rotation speed of 180rpm; immobilization time Obtained after immobilization under 18h.

[0041] Immobilization of soil polyphenol oxidase: Add mesoporous molecular sieve material SBA-15 to soil polyphenol oxidase, after immobilization at a temperature of 13°C; pH of 8; rotation speed of 180rpm; immobilization time of 18h get.

[0042] Preparation of the psychrotrophic bacterium Pseudomonas roserubinus HD1031 inoculum: culture the psychrophilic tolerant bacterium Pseudomonas roserubinus HD1031 to the logarithmic phase, and then inoculate the strains cultivated to the logarithmic phase according to the inoculation amount of 3% of the medium volume Into the culture medium, the medium is a medium with wheat straw powder and sweet potato vine powder as carbon source, ammonium sulfate and peptone as nitrog...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a microbial preparation for low-temperature soil repair. The compound microbial preparation comprises a cold-resistant pseudogymnoascus roseus HD1031 bactericide, an aerobic denitrifying cold-resistant acinetobacter johnsonii DBP-3 bactericide and immobilized oxidordeuctase, wherein the immobilized oxidordeuctase consists of immobilized horseradish peroxidase and immobilized soil polyphenol oxidase. The invention further discloses a preparation method and application of the microbial preparation. The microbial preparation for low-temperature soil repair has a removal rate of 86% or more of a pentazolol pesticide and an organophosphorus pesticide and a removal rate of 85% or more of ammonia nitrogen, which are much higher than removal rates in the prior art and a comparison example of the present invention. Thus, the microbial preparation for low-temperature soil repair can effectively repair polluted soil. The preparation method is simple and is suitable for large-scale production. Benefits are brought for remote low-temperature regions. The problem of environmental pollution that urgently needs to be solved is solved.

Description

[0001] Technical solutions [0002] The invention belongs to the technical field of soil remediation, and in particular relates to a microbial preparation for low-temperature soil remediation, a preparation method and application thereof. Background technique [0003] With the acceleration of urbanization and industrialization, soil organic pollution near cities and industrial areas is increasing day by day, and organic pollutants such as polycyclic aromatic hydrocarbons, pesticides, PCBs, and phthalates are in the soil around industrial areas Exceeding the national standard many times. At present, the overall situation of soil pollution in China is quite severe, which has already threatened the ecological environment, food safety, people's health and sustainable agricultural development. Pesticides are the largest and most toxic chemical substances that people actively put into the environment. After pesticides enter the soil, they mainly have two destinations: adsorption an...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/00B09C1/10C09K17/14A62D3/02C12N1/14C12N1/20C12R1/01C12R1/645C09K101/00
CPCA62D3/02A62D2101/04A62D2101/26B09C1/105C09K17/14C09K2101/00C12N1/14C12N1/20
Inventor 谢悦波姚竣耀刘才群
Owner 安徽中富恒源生物科技有限公司