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Application of a space station cell culture bottle and its auxiliary device

A technology for cell culture and auxiliary devices, which is applied in tissue cell/virus culture devices, biochemical cleaning devices, enzymology/microbiology devices, etc., to achieve good phenotype expression, maintain cell structure, and good cell structure.

Active Publication Date: 2022-03-11
天晴干细胞股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention solves the problem that there is currently no method for cultivating cells in a certain gravity environment that simulates a certain gravity environment in a specific space microgravity environment, and provides a method suitable for use in an aerospace on-orbit environment for space station cell culture

Method used

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  • Application of a space station cell culture bottle and its auxiliary device
  • Application of a space station cell culture bottle and its auxiliary device
  • Application of a space station cell culture bottle and its auxiliary device

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specific Embodiment approach 1

[0080] Specific implementation mode one: combine Figure 1 to Figure 13 Illustrate this embodiment, the application of a kind of space station cell culture bottle of this embodiment and its auxiliary device, it is used for cultivating cells derived from mammals, plant cells or microorganisms;

[0081] The cell culture flask comprises a culture flask body 1 and an end cap 3;

[0082] The culture bottle body 1 is a concentric cylinder composed of a plurality of culture bottles 4 with different diameters; each culture bottle 4 is a hollow cylinder; each culture bottle 4 is connected together by ribs 12;

[0083] Each culture bottle 4 is provided with a plurality of chambers in the side wall along the circumference as the culture chamber 2, and the culture bottle 4 is provided with multi-layer culture chamber 2 from top to bottom; , a hydrophobic and moisture barrier film is installed on the vent hole 1;

[0084] The end cap 3 is covered on the culture bottle body 1; the end cap...

specific Embodiment approach 2

[0103] Specific implementation mode two: combination Figure 1 to Figure 13 To illustrate this embodiment, the first difference between this embodiment and specific embodiments is that the cells derived from mammals are primary cells, passage cells, suspension cells or adherent cells. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0104] Specific implementation mode three: combination Figure 1 to Figure 13 Describe this embodiment, the difference between this embodiment and the specific embodiment is: it is to cultivate cells, plant cells or microorganisms derived from mammals according to the following method:

[0105] 1. Inoculate the culture medium containing cells into the culture bottle 4 from the A-way threaded joint 6 of the culture bottle body 1, and the cell-containing medium enters through the A-way perfusion main channel 9 and the A-way branch channel 11 and fills the adjacent In the culture room 2; enter the culture room 2 adjacent to the culture bottle 4 through the A-way branch channel 11; after the culture medium containing the cells can be seen from the B-way threaded joint 7, stop inoculation;

[0106] 2. Start the micro-motor 30 through the computer and the central controller, and drive the drive wheel 28 to control and drive the space station cell culture bottle 18 to rotate the cult...

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Abstract

The invention relates to an application of a space station cell culture bottle and its auxiliary device, which relates to an application of the space station cell culture bottle and its auxiliary device. The present invention aims to solve the problem that there is no method for cultivating cells in a specific environment of space microgravity simulating a certain gravity environment. In the present invention, the cell culture bottle of the space station is placed on an auxiliary device, and the cells are transported to the cell culture bottle of the space station through a threaded joint of the cell culture bottle of the space station to cultivate cells, plant cells or microorganisms derived from mammals. The present invention can simulate a certain gravitational environment through the high-speed rotating cell culture bottle, and make full use of the culture space and area through the rationally arranged culture bottle and cell chamber, increasing the surface area in the cell culture bottle that can carry out cell adherent culture, and can Simultaneous cultivation of multiple batches and types of cells reduces the experimental space and shortens the experimental time.

Description

technical field [0001] The invention relates to the application of a space station cell culture bottle and an auxiliary device thereof, and belongs to the field of space biological cell culture. Background technique [0002] The development of aerospace technology has made experts in life support technology and biology realize that the goal of manned spaceflight is not only short-term flights in low-earth orbit, but also lunar bases, Mars bases, and more distant deep space exploration and resident. The great goal must rely on the bio-regenerative life support system technology. In the closed micro-ecological circulation system artificially designed and constructed by scientists based on the characteristics of the space environment, biotechnology, especially cytology, undertakes the main task of life science. Cultivating cells in space can not only understand the changes in the metabolic activity of various cells in a microgravity environment, so as to control the changes in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00C12M1/24C12M1/04C12M1/00
CPCC12M23/08C12M29/20C12M41/48
Inventor 张怡张宇刘艳青
Owner 天晴干细胞股份有限公司
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