Preparation method of fish skin immunomodulatory peptides of Japanese croakers

Abstract

The invention relates to the technical field of preparation of polypeptides of Japanese croakers and particularly relates to a preparation method of fish skin immunomodulatory peptides of Japanese croakers. The preparation method comprises the following steps: performing degreasing on fish skin by lipase combined with isopropanol to eliminate the bitter taste of immunomodulatory peptides; then, treating the fish skin through tartaric acid, a pulsed electric field and a reductive amination reagent to weaken the crosslinking degree among fish skin molecular proteins and enhance the swelling of the fish skin proteins; dissolving out the fish skin proteins in the fish skin as much as possible by virtue of the action of papain, chymotrypsin, trypsin, aspergillus serine protease or / and alkali protease and other protein extraction enzymes with weaker decomposition effect on the fish skin proteins at the temperature of 50-60 DEG C; and then, decomposing the fish skin proteins by virtue of thecombined action of neutral protease and neutral metal protease to obtain the fish skin immunomodulatory peptides. The utilization ratio of the fish skin proteins is high, the chain breaking decomposition uniformity is high, and the molecular weight of the obtained immunomodulatory peptides is 2000-3500Da.

Description

technical field [0001] The invention relates to the technical field of polypeptide preparation of Japanese croaker, in particular to a method for preparing the skin immunomodulatory peptide of Japanese croaker. Background technique [0002] Japanese croaker is a kind of fish belonging to the genus crocodile in the family Crowfish. It is distributed in North Korea, southern Japan and coastal areas of China. At present, it has been introduced and cultured in Zhoushan fishery in Zhejiang Province. It has rapid seedling growth and disease resistance. Strong ability, short growth cycle, high nutritional value, economic benefits and other advantages. Japanese croaker will produce scraps such as fish skin, fish bones, and fish viscera during the processing process. In order to further increase the economic value of Japanese croaker and rationally utilize resources, it is necessary to develop and utilize the approach of Japanese croaker scraps . [0003] Immunomodulatory peptides ...

AI Technical Summary

Problems solved by technology

[0005] In view of the low utilization rate of fish skin protein in the current enzymatic hydrolysis method to obtain polypeptides from fish skin, the purpose of this application is to provide a method for preparing the skin immunomodulatory peptide of Japanese yellow croaker. Fish skin leftovers are used as raw materials to prepare fish skin immunomodulatory peptides. The preparation process is gentle. The fish skin protein in fish skin is fully utilized and the activity is guaranteed. The obtained immunomodulatory peptides have high preparation efficiency and good activity.