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A kind of method for extracting high-purity resveratrol

A resveratrol-specific technology, applied in the field of extracting high-purity resveratrol, can solve the problems of low purity and complicated resveratrol extraction process, and achieve high selectivity and the effect of eliminating the interference of impurities

Active Publication Date: 2020-11-10
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Based on this, it is necessary to provide a method for extracting high-purity resveratrol aiming at the technical problems that the extraction process of resveratrol is complicated and the purity is not high

Method used

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  • A kind of method for extracting high-purity resveratrol
  • A kind of method for extracting high-purity resveratrol
  • A kind of method for extracting high-purity resveratrol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] This example proposes a method for preparing a hydrophilic adsorption material that specifically recognizes resveratrol, comprising the following steps:

[0044] T1, measure 250mL of chloroform (an inert porogen), add 4.56g (20mmol) of 4'-chlororesveratrol (an alternative template molecule) and 6.40g (80mmol) of methacrylic acid (a A functional monomer), completely dissolved, and reacted for 10 minutes.

[0045] In other embodiments, the inert porogen can also be one or more of acetonitrile, toluene, chloroform and methanol; the functional monomer can also be methacrylic acid, acrylic acid, methylene succinic acid, p-vinyl One or more of benzoic acid, methacrylamide and N-isopropylacrylamide.

[0046] T2, add 10.40g (80mmol) of hydrophilic hydroxyethyl methacrylate (HEMA), 0.70g (4mmol) of N, N, N', N", N"-pentamethyldiethylenetriamine (PMDETA) and 0.90 g (4 mmol) of CuBr 2, then add 79.28g (400mmol) of ethylene glycol dimethacrylate (a cross-linking agent, the Engli...

Embodiment 2

[0054] This example proposes a method for preparing a hydrophilic adsorption material that specifically recognizes resveratrol, comprising the following steps:

[0055] T1. Measure 250 mL of acetonitrile, add 4.56 g (20 mmol) of azo-resveratrol and 11.77 g (80 mmol) of p-vinylbenzoic acid, dissolve completely, and react for 10 minutes.

[0056] T2, add 10.40g (80mmol) of hydrophilic hydroxyethyl methacrylate (HEMA), 0.70g (4mmol) of N, N, N', N", N"-pentamethyldiethylenetriamine (PMDETA) and 0.90g (4mmol) of CuBr2, then add 52.08g (400mmol) of DVB and 0.36g (4mmol) of tert-butyl hydroperoxide, react with nitrogen gas at 0-4°C for 30 minutes, seal, After reacting in a water bath at 50° C. for 48 hours, the polymer obtained by the reaction precipitated.

[0057] T3. Soxhlet extraction was performed with 250 mL of glacial acetic acid-ethanol solution (10% by volume of glacial acetic acid), and the extraction was repeated 6 times for 6 hours each time. The collected precipitate ...

Embodiment 3

[0063] Extraction of trans-resveratrol crystalline monomers from thorn grape seeds:

[0064] This example proposes a method for extracting resveratrol crystalline monomers using the hydrophilic adsorption material that specifically recognizes resveratrol prepared in Example 1, including the following steps:

[0065] S1. Accurately weigh 50g of crushed and sieved grape seeds, specifically grape seeds, into a 250mL conical flask with stopper, add 50mL of deionized water at a temperature of 90-100°C and 10g of hydrophilic adsorption material, and stir Evenly, add 10mL of 100mg / L ascorbic acid solution, sonicate for 10 minutes, and transfer the mixture thoroughly to a glass column with an inner diameter of 2cm (with piston, sieve plate and ground plug). The thorn grape seeds are directly crushed, and the resveratrol is extracted by deionized water with a high temperature. The hydrophilic adsorption material can absorb the resveratrol molecules in the mixed solution and retain them...

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Abstract

The invention relates to a method for extracting high-purity resveratrol, comprising the steps of: 1) uniformly mixing raw materials containing resveratrol and a hydrophilic adsorption material that specifically recognizes resveratrol, and filling them into an adsorption column; 2) washing the adsorption column with deionized water, and discarding the washing liquid; 3) eluting the adsorption column with an acetonitrile solution containing 1 to 10% organic acid, and collecting the eluate; or, further The method comprises the following steps: 4) concentrating the obtained eluent, redissolving into a supersaturated solution, adding pure resveratrol as a seed crystal, and standing to crystallize, the method is simple and the obtained resveratrol has high purity, The highest purity is 98.38%.

Description

technical field [0001] The invention relates to the technical field of food deep processing, in particular to a method for extracting high-purity resveratrol. Background technique [0002] Because the content of resveratrol in plants is very low, the cost of extracting resveratrol from plants and obtaining resveratrol by biosynthesis is too high and the process is complicated; and the use of chemical methods to synthesize resveratrol has become the main focus of its development. means. However, chemical synthesis methods generally have many shortcomings such as complex routes, high safety risks, low yields, complex by-products, low purity, chemical residues, and food risks. In addition, because trans-resveratrol is unstable, it will be converted into cis-resveratrol under natural light, and its activity will be greatly reduced. It is difficult to obtain high-purity trans-resveratrol with a single structure by chemical synthesis. . Contents of the invention [0003] Base...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F220/20C08F220/06C08F222/14C08F212/14C08F212/36C08J9/28B01J20/26C07C37/70C07C37/82C07C39/21
CPCC07C37/685C07C37/70C07C37/82C08F220/20B01J20/261C08J2201/0502C08J2333/14C07C39/21C08F220/06C08F222/102C08F212/14C08F212/36
Inventor 谭君杨国顺白描彭淼王美军
Owner HUNAN AGRICULTURAL UNIV
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