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Paddy field nitrogen-fixing cyanobacteria producing salicylic acid and application of paddy field nitrogen-fixing cyanobacteria to paddy fields

A technology of nitrogen-fixing cyanobacteria and salicylic acid, applied in the direction of application, microbial-based methods, biochemical equipment and methods, etc., to achieve the effects of increasing lodging and disease resistance, fast growth, and strong nitrogen and carbon fixation capabilities

Active Publication Date: 2018-11-06
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, studies at home and abroad have shown that nitrogen-fixing cyanobacteria can produce a variety of plant hormones including auxin and cytokinin, but there is no report on the production of salicylic acid by nitrogen-fixing cyanobacteria in paddy fields.

Method used

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  • Paddy field nitrogen-fixing cyanobacteria producing salicylic acid and application of paddy field nitrogen-fixing cyanobacteria to paddy fields
  • Paddy field nitrogen-fixing cyanobacteria producing salicylic acid and application of paddy field nitrogen-fixing cyanobacteria to paddy fields
  • Paddy field nitrogen-fixing cyanobacteria producing salicylic acid and application of paddy field nitrogen-fixing cyanobacteria to paddy fields

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Separation, purification and identification of nitrogen-fixing cyanobacteria SCAU-003

[0031] (1) Separation and purification of algae species

[0032] Surface soil sample: collected from the paddy field of Linxi Base in Zengcheng, South China Agricultural University.

[0033] Separation and purification of algae: take soil sample and add 20mL BG11 at 1g 0 In the liquid medium, pre-culture for 7 days in a light incubator at a temperature of 28°C, a light of 3000lx, and a light and dark time of 16h:8h. Then take the upper layer of clear liquid containing algae and spread it on 9cm containing BG11 0 On a plate with solid medium. After the bacteria that are visible to the naked eye grow, carefully pick out and re-scratch the plate and check it under microscope. This process is repeated more than 3 times to obtain a single culture of nitrogen-fixing cyanobacteria. Finally, a sterile algae strain is obtained through combined treatment of ultraviolet radiation and mult...

Embodiment 2

[0053] Example 2: Evaluation of SCAU-003 growth, nitrogen fixation, carbon fixation and biomass capacity

[0054] Use the above BG11 0 After the liquid medium is inoculated to the logarithmic growth phase of the algae SCAU-003, in a light incubator, the temperature is 28℃, the light is 3000lx, and the light and dark time is 16h:8h. The growth curve of the algae strain SCAU-003 is as follows Figure 5 Shown, OD 680 The value increased from 0.08 at the time of vaccination to 0.65 on the 13th day, an increase of more than 5 times. The calculation results show that the algae has a higher specific growth rate (μ = 0.163d -1 ).

[0055] The algae were cultivated in the same way, and samples were taken on the 4th day. The algae liquid was taken to determine the nitrogenase activity by acetylene reduction-gas chromatography. The measurement results showed that the nitrogenase activity of the algae was 0.309±0.032μmol C 2 H 2 ·H -1 ·(Μg Chla) -1 . On the 15th day, samples were taken to col...

Embodiment 3

[0057] Example 3: Evaluation of SCAU-003's ability to produce salicylic acid and other plant hormones

[0058] SCAU-003 algae in BG11 0 Cultured in the medium for 20 days, centrifuged to get the algae cells to freeze-dry. The supernatant was filtered with a 0.22 micron filter membrane. The algae cells were ground and broken in liquid nitrogen and added 1ml Bieleski buffer (60% methanol, 25% chloroform, 10% formic acid and 5% water) containing an internal standard (5mg / L DHZ), ultrasound (frequency 20kHz, Power is 200W, working / intermittent is 30s / 30s) 3min to fully extract. The supernatant was collected by centrifugation, and the precipitate was extracted with 0.3ml of a solution containing 2% formic acid and 50% methanol by ultrasound for 3 minutes again. The supernatant was collected by centrifugation, combined with the supernatant from the first extraction, and rotary evaporated to dryness at 40°C under reduced pressure Afterwards, the volume was adjusted to 1 mL with a meth...

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Abstract

The invention discloses paddy field nitrogen-fixing cyanobacteria producing salicylic acid and application of the paddy field nitrogen-fixing cyanobacteria to paddy fields. The name of the strain is Nostoc piscinale SCAU-003; the preservation number of the strain is CCTCC NO: M 2018194; the preservation date of the strain is April 11, 2018; the preservation organization is in China Center for TypeCulture Collection in Wuhan University, Wuhan, China. The strain can produce plant hormone, such as high-concentration salicylic acid, is fast to grow and high in nitrogen-fixing and carbon-fixing capacity, can serve as biofertilizer to be applied to paddy fields through a novel applying mode, can replace half of chemical nitrogenous fertilizer, and improves the lodging-resistant and disease-resistant capacity of paddies.

Description

Technical field [0001] The invention relates to the field of microorganisms and the field of application of microbial fertilizers, in particular to a nitrogen-fixing cyanobacteria that produces salicylic acid and its application in the paddy field. Background technique [0002] After Indian scientists first reported using cyanobacteria to fertilize fields in 1939, many countries have successively carried out related studies, and studies have shown that inoculation of cyanobacteria in rice fields can increase production. Since the 1950s, Li Shanghao has conducted experiments on algae cultivation in paddy fields in some southern provinces of my country, and the results show that rice production has increased by 10-30%. Nitrogen-fixing cyanobacteria can not only provide nitrogen and organic matter for crops, increase yield, reduce fertilizer consumption, increase phosphate solubility, reduce water and soil pollution, but also secrete plant hormones to promote crop growth and other b...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05G3/00C12R1/89
CPCC05F11/00C05G3/00C12N1/20C12N1/125C12R2001/89
Inventor 贺鸿志包江桥周伊薇卢玉真陈桂葵黎华寿秦俊豪
Owner SOUTH CHINA AGRI UNIV
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