Yeast-source functional protein and preparation method thereof
A functional protein and yeast technology, which is applied in the field of yeast-derived functional protein preparation, can solve the problems of lack of functional components in fermented soybean meal, and achieve the effects of improving efficiency, reducing cost and improving fermentation effect.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
preparation example Construction
[0027] The present invention is a preparation method of yeast-derived functional protein, comprising the following steps:
[0028] (1) Centrifuge the brewer's yeast sludge, and then collect the yeast cells.
[0029] (2) adding the yeast cells collected in the step (1) into sterile water to suspend to obtain a yeast suspension containing 200-500 g / L of yeast dry matter.
[0030] (3) Add the yeast suspension obtained in step (2) to the enzymolysis tank, and add enzyme preparation and high temperature resistant yeast agent to the enzymolysis tank, at a temperature of 42°C to 45°C and a rotating speed of 50rpm Under the condition of ~100rpm, the enzyme is hydrolyzed for 3h ~ 10h to obtain the yeast hydrolyzate. Preferably, the enzyme preparation added is a mixture of cellulase, β-glucanase, β-mannanase and protease; In terms of the total mass percentage of the liquid, they are: 0.001% to 2% of cellulase, 0.001% to 2% of β-glucanase, 0.001% to 2% of β-mannanase, and 0.001% to 2% ...
Embodiment 1
[0038] Yeast slime was taken from Zhanjiang Zhujiang Brewery Co., Ltd., the yeast slime was centrifuged, and then the yeast cells were collected; the collected yeast cells were suspended in sterile water to obtain a yeast suspension containing 300 g / L of yeast dry matter ( 99% of the yeast cell walls in the bacterial suspension are intact and not broken). Add the yeast suspension to the enzymolysis tank, then add 0.01% cellulase, 0.02% β-glucanase, 0.03% β-mannanase and 0.02% Protease, then add 0.02% high-temperature-resistant yeast agent (produced by Hubei Angel Yeast Co., Ltd.), enzymolysis 8h under the condition of 43 ℃ and rotating speed at a temperature of 70rpm, to obtain yeast hydrolyzate (the rate of wall breaking of yeast 92%); 1000kg of yeast hydrolyzate and 1000kg of soybean meal crushed through a 60-mesh sieve are mixed, then mixed with 2kg of Bacillus subtilis inoculum; Cover and seal the fermented materials in the pile with plastic film. The fermentation tempera...
Embodiment 2
[0040]Yeast slime was taken from Zhanjiang Zhujiang Brewery Co., Ltd., the yeast slime was centrifuged, and then the yeast cells were collected; the collected yeast cells were suspended in sterile water to obtain a yeast suspension containing 400 g / L of yeast dry matter ( 99% of the yeast cell walls in the bacterial suspension are intact and not broken). Add the yeast suspension to the enzymolysis tank, then add 0.02% cellulase, 0.03% β-glucanase, 0.04% β-mannanase and 0.03% Protease, then add 0.02% high-temperature-resistant yeast agent (produced by Hubei Angel Yeast Co., Ltd.), enzymolysis 8h under the condition of 42 ℃ and rotating speed at a temperature of 80rpm, to obtain a yeast hydrolyzate (the wall breaking rate of yeast 91%); 900kg of yeast hydrolyzate and 1000kg of soybean meal crushed through a 60-mesh sieve are mixed, then inserted into 2kg of Bacillus subtilis inoculum and mixed evenly; 130cm, and cover the piled fermentation material with a plastic film and seal...
PUM
| Property | Measurement | Unit |
|---|---|---|
| Broken rate | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More