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Method or culturing microalgae through membrane aeration

A membrane aeration, microalgae technology, applied in the directions of microorganism-based methods, chemical instruments and methods, biochemical equipment and methods, etc., to achieve the effects of being beneficial to the growth of microalgae, increasing the degree of photosynthesis, and increasing the utilization rate

Inactive Publication Date: 2018-11-13
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Targeting CO in fossil fuel combustion exhaust 2 The problem of excess emissions, regarding the fixation of CO by microalgae 2 Relevant researches have also been carried out since the late 20th century, but most of the research is on the adsorption of carbon dioxide in the air by microalgae, and only a few use artificial experimental conditions to match relatively high concentrations of carbon dioxide to test the tolerance of microalgae to carbon dioxide. Research

Method used

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  • Method or culturing microalgae through membrane aeration

Examples

Experimental program
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Embodiment 1

[0031] A method utilizing membrane aeration to cultivate microalgae, said method comprising the steps of:

[0032] Source of algae species: select chlorella that has been transformed into algae for 5 days in a constant temperature light incubator, and add 10mL of algae per 100mL of culture solution;

[0033] Experimental vial: the vial is a 1L glass bottle with a blue cap, and the working volume is 660mL (that is, 60mL of algae solution + 600mL of culture solution);

[0034] Vial reaction conditions: control the internal temperature of the incubator at 25°C, the light intensity at 8333 lumens, the initial pH of the culture solution at 7.0, the hollow fiber membrane is polyvinylidene fluoride, the pore size of the hollow fiber membrane is 300nm, and the membrane area of ​​the hollow fiber membrane is 14.57cm 2 , the concentration of carbon dioxide gas is 20%;

[0035] Batch test operation mode: the reaction time of each batch of vial experiments is 6 days, water samples are t...

Embodiment 2

[0037] A method for cultivating microalgae using membrane aeration, the difference from Example 1 is that the pore diameter of the hollow fiber membrane is 100nm.

Embodiment 3

[0039] A method for cultivating microalgae using membrane aeration, the difference from Example 1 is that the pore diameter of the hollow fiber membrane is 30nm.

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Abstract

The invention relates to a method or culturing a micro alga through membrane aeration and belongs to the technical field of micro alga culture. The micro alga is chlorella. A culture liquid comprises1.5g / L sodium nitrate, 0.04g / L dipotassium phosphate, 0.075g / L magnesium sulfate, 0.036g / L calcium chloride, 0.006g / L citric acid, 0.006g / L ammonium ferric citrate, 0.01g / L ethylenediamine tetraaceticacid disodium and 1mL / L a trace element solution, and a pH value is 7.0. By adopting the method, culture conditions are optimized, the purposes that carbon dioxide bubbles are reduced and the utilization rate of carbon dioxide is increased are achieved through hollow fiber membrane aeration, meanwhile, the growth speed of the micro alga is increased, and then an economic and effective novel technique is provided for culture of the micro alga and utilization of the carbon dioxide.

Description

technical field [0001] The invention relates to a method for cultivating microalgae by using membrane aeration, and belongs to the technical field of microalgae cultivation. Background technique [0002] Microalgae reproduce rapidly, can consume a large amount of nutrients, and consume carbon dioxide to release oxygen through photosynthesis; they are widely distributed, have no strict requirements on environmental conditions, and have strong adaptability; they also have the functions of decomposition, oxidation, filtration, and adsorption. With the development of microalgae culture technology, in order to reduce the energy consumption and cost of microalgae culture, microalgae fix CO 2 The technical coupling with sewage treatment has become a current research hotspot. Targeting CO in fossil fuel combustion exhaust 2 The problem of excess emissions, regarding the fixation of CO by microalgae 2 Relevant researches have also been carried out since the late 20th century, but ...

Claims

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Application Information

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IPC IPC(8): C12N1/12C02F3/32C12R1/89
CPCC02F3/322C12N1/12
Inventor 乔森田奕晖金若菲周集体
Owner DALIAN UNIV OF TECH
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