Lactobacillus plantarum JYLP-002, products prepared thereby and application of lactobacillus plantarum JYLP-002
A technology of JYLP-002, Lactobacillus plantarum, applied in biochemical equipment and methods, microorganism-based methods, medical preparations with non-active ingredients, etc., can solve the problems of slow effect, side effects, etc. The effect of improving uncomfortable symptoms and avoiding the risk of drug resistance
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Embodiment 1
[0028] Screening of dominant lactic acid bacteria
[0029] ①Bacteria source: vaginal secretions of healthy young women
[0030] ② Plate medium: peptone 10g, beef extract powder 8.6g, yeast extract powder 5g K 2 HPO 4 2g triammonium citrate 2g sodium acetate 5g glucose 20g Tween 80 1mL MgSO 4 ·7H 2 O 0.58g MnSO 4 4H 2 O 0.25g agar 15g distilled water 1000mL
[0031] ③Isolation of strains
[0032] The gynecological medical examiner of Qingzhou Central Hospital assisted in sampling, and swabbed the rear 1 / 3 of the vagina with a sterile cotton swab in a clockwise direction, avoiding contact with the vulva and vaginal opening to prevent contamination. Immediately put it into sterile water after taking it out, and record the number.
[0033] The sample liquid was diluted tenfold, and 100 μL of each gradient was evenly spread on the MRS solid plate medium, then taken out and placed in an anaerobic tank, and incubated overnight at 37°C.
[0034] The next day, colonies of dif...
Embodiment 2
[0043] Comparison of the pH value drop rate during the cultivation of different strains
[0044] The ability to reduce the pH value (after 16h of MRS medium fermentation) is shown in Table 1:
[0045] Table 1
[0046] bacteria
pH value
bacteria
pH value
JYLP-002
3.65
ATCC4357
5.17
CICC22700
4.12
CICC6236
4.12
ATCC39392
3.98
ATCC8014
4.04
CICC6126
4.62
ATCC11443
4.23
[0047] Other strains in the table are from ATCC and CICC Culture Collection.
[0048] Conclusion: It can be seen from Table 1 that the JYLP-002 strain has the fastest pH drop under the same time and conditions, and has stronger acid production ability than other strains.
[0049] Fermentation of different strains grows faster and slower. (The initial pH value of MRS medium at 37°C is 6.2-6.4 to the peak time of fermentation, and the detection data of each bacteria is the average value after 10 measurements) as shown i...
Embodiment 3
[0055] Inhibit the growth of harmful bacteria (co-cultivation) (Gardnerella is the main pathogenic bacteria of bacterial vaginosis, Candida albicans is the main pathogenic bacteria of Candida vaginitis)
[0056] Gardnerella: ATCC 14018
[0057] Co-cultivation medium: MRS medium
[0058] JYLP-002 initial inoculum volume: 10 6 cfu / mL
[0059] Gardnerella initial inoculum size: 10 6 cfu / mL
[0060] Instructions attached figure 1 is the co-culture curve.
[0061] Conclusion: JYLP-002 can significantly inhibit the growth of Gardnerella in liquid environment
[0062] Candida albicans: CICC 1965
[0063] Co-cultivation medium: 5°Bé wort 1.0L
[0064] Initial inoculum volume of JYLP-002: 106cfu / mL
[0065] Initial inoculation volume of Candida albicans: 106cfu / mL.
[0066] Instructions attached figure 2 is the co-culture curve.
[0067] Conclusion: JYLP-002 can significantly inhibit the growth of Candida albicans in liquid environment.
[0068] Inhibition zone of pathoge...
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