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A kind of Lactobacillus plantarum jylp-002 and the product and application thereof

A technology of JYLP-002 and Lactobacillus plantarum, applied in biochemical equipment and methods, methods based on microorganisms, medical preparations with non-active ingredients, etc., can solve problems such as side effects and slow effect, and improve discomfort symptoms , Eliminate peculiar smell, reduce the effect of recurrence probability

Active Publication Date: 2019-06-28
WEIFANG ZHONGKEJIAYI BIO FODDER TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the effect of external application of traditional Chinese medicine and the dosage are not as obvious as that of oral administration. However, oral administration of traditional Chinese medicine still has side effects, and the effect is slow

Method used

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  • A kind of Lactobacillus plantarum jylp-002 and the product and application thereof
  • A kind of Lactobacillus plantarum jylp-002 and the product and application thereof
  • A kind of Lactobacillus plantarum jylp-002 and the product and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Screening of dominant lactic acid bacteria

[0030] ①Bacteria source: vaginal secretions of healthy young women

[0031] ② Plate medium: peptone 10g beef extract powder 8.6g yeast extract powder 5g K 2 HPO 4 2g Triammonium Citrate 2g Sodium Acetate 5g Glucose 20g Tween 80 1mL MgSO 4 ·7H 2 O 0.58g MnSO 4 4H 2 O 0.25g agar 15g distilled water 1000mL

[0032] ③Isolation of strains

[0033] The gynecological medical examiner of Qingzhou Central Hospital assisted in sampling, and swabbed the rear 1 / 3 of the vagina with a sterile cotton swab in a clockwise direction, avoiding contact with the vulva and vaginal opening to prevent contamination. Immediately put it into sterile water after taking it out, and record the number.

[0034] The sample liquid was diluted tenfold, and 100 μL of each gradient was evenly spread on the MRS solid plate medium, then taken out and placed in an anaerobic tank, and incubated overnight at 37°C.

[0035] The next day, colonies of diffe...

Embodiment 2

[0044] Comparison of the pH value drop rate during the cultivation of different strains

[0045] The ability to reduce the pH value (after 16h of MRS medium fermentation) is shown in Table 1:

[0046] Table 1

[0047] bacteria

pH value

bacteria

pH value

JYLP-002

3.65

ATCC4357

5.17

CICC22700

4.12

CICC6236

4.12

ATCC39392

3.98

ATCC8014

4.04

CICC6126

4.62

ATCC11443

4.23

[0048] Other strains in the table are from ATCC and CICC Culture Collection.

[0049] Conclusion: It can be seen from Table 1 that the JYLP-002 strain has the fastest pH drop under the same time and conditions, and has stronger acid production ability than other strains.

[0050] Fermentation of different strains grows faster and slower. (The initial pH value of MRS medium at 37°C is 6.2-6.4 to the peak time of fermentation, and the detection data of each bacteria is the average value after 10 measurements) as shown i...

Embodiment 3

[0056] Inhibit the growth of harmful bacteria (co-cultivation) (Gardnerella is the main pathogenic bacteria of bacterial vaginosis, Candida albicans is the main pathogenic bacteria of Candida vaginitis)

[0057] Gardnerella: ATCC 14018

[0058] Co-cultivation medium: MRS medium

[0059] JYLP-002 initial inoculum volume: 10 6 cfu / mL

[0060] Gardnerella initial inoculum size: 10 6 cfu / mL

[0061] Instructions attached figure 1 is the co-culture curve.

[0062] Conclusion: JYLP-002 can significantly inhibit the growth of Gardnerella in liquid environment

[0063] Candida albicans: CICC 1965

[0064] Co-cultivation medium: 5°Bé wort 1.0L

[0065] Initial inoculum volume of JYLP-002: 106cfu / mL

[0066] Initial inoculation volume of Candida albicans: 106cfu / mL.

[0067] Instructions attached figure 2 is the co-culture curve.

[0068] Conclusion: JYLP-002 can significantly inhibit the growth of Candida albicans in liquid environment.

[0069] Inhibition zone of pathoge...

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Abstract

The invention discloses Lactobacillus plantarum JYLP-002 and its prepared product and application. The preserved strain Lactobacillus plantarum JYLP‑002 is used to make external and internal products for joint use. It can not only relieve local inflammation, inhibit pathogenic bacteria, colonize the vaginal wall, produce antibacterial substances, eliminate peculiar smell, and maintain the balance of environmental flora through external application; symptoms of discomfort. The risk of drug resistance caused by the use of antibiotics is avoided, and the gastrointestinal damage caused by the use of antibiotics to the body is avoided. Improve the cure rate of vaginitis and reduce the probability of vaginitis recurrence.

Description

technical field [0001] The invention relates to the technical field of Lactobacillus plantarum and its application, in particular to a Lactobacillus plantarum JYLP-002 and its prepared product and application. Background technique [0002] The current treatment methods for vaginitis are as follows: [0003] 1. Antibiotic treatment. Antibiotics are effective in treatment, but their disadvantages are also significant. The use of antibiotics will kill both pathogenic and beneficial bacteria. Although it will temporarily relieve symptoms, it will seriously lead to a lack of vaginal flora, which can easily cause recurrence. Overuse of antibiotics can lead to resistance of harmful bacteria. At the same time, long-term use of antibiotics can cause gastrointestinal discomfort, low immunity, etc., and induce other diseases. [0004] 2. Chinese medicine treatment. The methods of treating vaginitis with traditional Chinese medicine are complicated, various in type, slow in effect a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/747A61K9/19A61K9/00A61P15/02C12R1/25
CPCA61K9/0034A61K9/19A61K47/26A61K2035/115A61P15/02C12N1/20C12N1/205C12R2001/25
Inventor 潘玉林曹维超马明磊司书锋闫洪伟王维新
Owner WEIFANG ZHONGKEJIAYI BIO FODDER TECH
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