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A droplet chip cell analysis system and its analysis method

An analysis system and chip technology, applied in the field of medical devices, to achieve the effects of low reagent consumption, saving test costs, and improving work efficiency

Active Publication Date: 2021-07-02
GUANGZHOU FIRST PEOPLES HOSPITAL (GUANGZHOU DIGESTIVE DISEASE CENT GUANGZHOU FIRST PEOPLES HOSPITAL GUANGZHOU MEDICAL UNIV THE SECOND AFFILIATED HOSPITAL OF SOUTH CHINA UNIV OF TECH)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, there is no integrated system that can complete all cell analysis steps

Method used

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  • A droplet chip cell analysis system and its analysis method
  • A droplet chip cell analysis system and its analysis method
  • A droplet chip cell analysis system and its analysis method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A droplet chip cell analysis system, comprising: an installation platform as a basic installation plane, a sample carrier platform for carrying cell analysis samples, a reagent carrier platform for cell analysis reagents, a chip carrier platform for open chips, and a sample carrier platform on the sample carrier platform , Reagent carrying platform, chip carrying platform between the sampling needle for micro pipetting, the temperature control module for temperature control of the chip, the optical detection module for cell analysis of the contents in the chip, and the signal acquisition for the optical detection module The signal acquisition module, the spatial translation mechanism, the linear translation mechanism, and the dark chamber where the optical detection module is installed; the sample carrier, reagent carrier, chip carrier, sampling needle, temperature control module, optical detection module, and signal acquisition module are all integrated in the On the in...

Embodiment 2

[0040] A droplet chip cell analysis system, comprising: an installation platform as a basic installation plane, a sample carrier platform for carrying cell analysis samples, a reagent carrier platform for cell analysis reagents, a chip carrier platform for open chips, and a sample carrier platform on the sample carrier platform , Reagent carrying platform, chip carrying platform between the sampling needle for micro pipetting, the temperature control module for temperature control of the chip, the optical detection module for cell analysis of the contents in the chip, and the signal acquisition for the optical detection module The signal acquisition module, the plane translation mechanism, the rotating lifting arm, and the dark chamber where the optical detection module is installed; the sample carrier, reagent carrier, chip carrier, sampling needle, temperature control module, optical detection module, and signal acquisition module are all integrated in the On the installation...

Embodiment 3

[0045] The analysis system of Embodiment 1 is used to realize the bacterial antibiotic susceptibility test based on the color reaction on the glass chip with patterned hydrophilic dots.

[0046] The experiment uses a glass material chip with a size of 5×10cm. The chip has a group of 6×12 hydrophilic lattices, each hydrophilic dot has a diameter of 3mm, and the area around the hydrophilic dot is treated with hydrophobic and oleophobic coating.

[0047] Specific steps are as follows:

[0048] (1) The sampling needle sucks 5 μL of FC-40 fluorinated oil and 5 μL of urine sequentially, and loads them on the hydrophilic spots of the column, forming a total of 72 droplets in 6 rows and 12 columns.

[0049] (2) 5 μL of culture medium containing six concentrations (0-5) of one antibiotic was added to each row of droplets. There were 12 kinds of antibiotics tested, including: erythromycin, clindamycin, ampicillin, vancomycin, gentamicin, ciprofloxacin, levofloxacin, imipenem, cefazoli...

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Abstract

The invention discloses a droplet chip cell analysis system, comprising: an installation platform serving as a basic installation plane, a sample carrying platform carrying a cell analysis sample, a reagent carrying platform carrying a cell analysis reagent, a chip carrying platform carrying an open chip, The sampling needle for translating micropipetting between the sample carrier, reagent carrier, and chip carrier, the temperature control module for temperature control of the chip, the optical detection module for cell analysis of the contents in the chip, and the optical detection module A signal acquisition module for signal acquisition. It also includes an analysis method of the droplet chip cell analysis system. The liquid droplet chip cell analysis system based on the micro-droplet technology provided by the present invention integrates the whole process of certain cell analysis, has the advantages of automatic operation, fast analysis speed, and high test throughput, and belongs to the medical device for biological detection.

Description

technical field [0001] The invention relates to a medical device for biological detection, in particular to a droplet chip cell analysis system and an analysis method thereof. Background technique [0002] Compared with traditional biological analysis using test tubes as carriers, droplet analysis has many advantages: (1) The droplet reactor is small in size, which can significantly save reagent consumption; (2) It is easy to achieve high-throughput analysis; (3) Benefiting from the shortening of the reaction time in the microsystem, the analysis time can be significantly saved; (4) the water phase sample is wrapped by the oil phase, which eliminates the influence of evaporation and surface adsorption factors on the analysis results. [0003] Typical cell analysis steps include: cell seeding, cell culture, cell stimulation, and cell response detection. In the prior art, there is no integrated system that can complete all cell analysis steps. Contents of the invention [...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N35/00G01N35/10G01N21/64
CPCG01N21/6458G01N35/00584G01N35/1011G01N2035/1037
Inventor 刘大渔
Owner GUANGZHOU FIRST PEOPLES HOSPITAL (GUANGZHOU DIGESTIVE DISEASE CENT GUANGZHOU FIRST PEOPLES HOSPITAL GUANGZHOU MEDICAL UNIV THE SECOND AFFILIATED HOSPITAL OF SOUTH CHINA UNIV OF TECH)