Preparation method of activated Agrocybe cylindracea liquid strain

A technology of liquid strains and camellia oleifera mushrooms is applied in the fields of botanical equipment and methods, mushroom cultivation, horticulture, etc., which can solve the problems of low strain concentration, few germination points of mycelium, long harvesting period, etc. The effect of shortening the fruiting time

Inactive Publication Date: 2018-11-20
贵州省贵福菌业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The time for artificial domestication and successful cultivation of Camellia oleifera is very short. Although the output of artificial cultivation has increased greatly in recent years, due to its long harvest cycle and relatively cumbersome process, it is still in the primary bag cultivation stage. The impact is great, and large-scale factory cultivation has not been realized, and the strains are still traditional solid strains. Since most solid strains are in the dormant period, the culture time of the strains is long, and each strain bottle can only be inoculated with a few at most. Ten cultivation bags need to prepare a large number of strain bottles, and after inoculation, the cultivation bags have few mycelial germination points, the germination speed is slow, and miscellaneous fungus and insect damage are prone to occur. The cultivation period is long, and industrial cultivation is difficult to achieve.
[0003] Therefore, in the prior art, the seed entity of Camellia oleifera will be first activated and liquid expanded culture, such as the invention patent disclosed in the patent CN201610472477.9 "A kind of culture medium of Camellia oleifera based on Juncao and preparation of liquid strains of Camellia oleifera Method", which uses Juncao, carrot powder, yeast autodissolving powder, royal jelly, potassium dihydrogen phosphate, magnesium sulfate, gypsum powder, sophorolipids, calcium propionate, plant hydrogenated oil, sodium hydroxymethyl cellulose, The culture medium of cellulase, light calcium carbonate, nano-scale tourmaline powder, and bacterial nanocellulose is used to expand the culture of the strain, such as the invention patent disclosed in the patent CN201510587382. Preparation method", the above technology also plays a certain role, but the culture time is too long, and the concentration of the cultured bacteria is too low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] This embodiment provides a kind of preparation method of oleifera oleifera activated liquid spawn, comprising the following steps:

[0019] 1. Medium preparation

[0020] 1) In the activation medium: 15g / L potato juice, 40g / L agar and 4g / L ginkgo leaf extract, ginkgo leaf extract contains 20-30% ginkgo flavonoids and 2-8% lactone, ginkgo leaf extract purchased from Xi'an Best Biological Technology Co., Ltd.

[0021] 2) Fermentation liquid medium: 10g / L wheat flour, 30g / L carrot powder, 35g / L shell powder, 5g / L ginkgo leaf extract, 0.4g / L potassium dihydrogen phosphate, 20g / L lignocellulose, 0.3g / L magnesium sulfate, 20g / L sugar, 2g / L yeast powder. The shell powder is calcined at 560-575°C for 1 hour in an internal environment of 0.03 atmospheres, then ground into powder, sprayed with 10% sodium hydroxide solution, and kept at 200°C for 5 hours, then adjusting the pH value to 6 with phosphoric acid. -6.5 is enough.

[0022] 2. Preparation of activated liquid strains...

Embodiment 2

[0026] This embodiment provides a kind of preparation method of oleifera oleifera activated liquid spawn, comprising the following steps:

[0027] 1. Medium preparation

[0028] 1) In the activation medium: 45g / L potato juice, 20g / L agar and 10g / L ginkgo leaf extract, ginkgo leaf extract contains 20-30% ginkgo flavonoids and 2-8% lactone, ginkgo leaf extract purchased from Xi'an Best Biological Technology Co., Ltd.

[0029] 2) Fermentation liquid medium: 20g / L wheat flour, 20g / L carrot powder, 45g / L shell powder, 0.2g / L ginkgo leaf extract, 0.5g / L potassium dihydrogen phosphate, 10g / L lignocellulose, 0.4 g / L magnesium sulfate, 10g / L sugar, 2g / L yeast powder. The shell powder is calcined at 560~575°C for 3 hours in an internal environment of 0.08 atmospheric pressure, then ground into powder, sprayed with 10-15% sodium hydroxide solution, and kept at 200~300°C for 3~5 hours, and then used Phosphoric acid can be used to adjust the pH to 6-6.5.

[0030] 2. Preparation of acti...

Embodiment 3

[0034] This embodiment provides a kind of preparation method of oleifera oleifera activated liquid spawn, comprising the following steps:

[0035] 1. Medium preparation

[0036] 1) In the activation medium: 20g / L potato juice, 35g / L agar and 7g / L Ginkgo biloba extract, Ginkgo biloba extract contains 20-30% Ginkgo flavonoids and 2-8% lactone, Ginkgo biloba extract purchased from Xi'an Best Biological Technology Co., Ltd.

[0037] 2) Fermentation liquid medium: 15g / L wheat flour, 27g / L carrot powder, 38g / L shell powder, 2g / L ginkgo leaf extract, 0.45g / L potassium dihydrogen phosphate, 15g / L lignocellulose, 0.33g / L magnesium sulfate, 15g / L sugar, 4g / L yeast powder. The shell powder is calcined at 560~575°C for 1~3 hours in an internal environment of 0.03~0.08 atmospheres, then ground into powder, sprayed with 10-15% sodium hydroxide solution, and heated at 200~300°C for 3~3 hours. 5h, and then adjust the pH value to 6-6.5 with phosphoric acid.

[0038] 2. Preparation of acti...

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Abstract

The invention discloses a preparation method of an activated Agrocybe cylindracea liquid strain. The preparation method comprises activated culture of Agrocybe cylindracea fruiting bodies and fermented culture. An adopted activation medium containing ginkgo leaf extract can rapidly activate the Agrocybe cylindracea fruiting bodies, by means of culture with a fermented liquid medium and added ginkgo leaf extract and shell powder, the Agrocybe cylindracea strain can be propagated rapidly by chitin contained in the shell powder, fruiting time of inoculated Agrocybe cylindracea is shortened, and yield of Agrocybe cylindracea is increased.

Description

technical field [0001] The invention belongs to the field of edible fungi, and in particular relates to a preparation method of an activated liquid strain of Camellia oleifera. Background technique [0002] Camellia oleifera, columnar field head mushroom, is a kind of edible and medicinal fungus. It has a delicate cap, a crisp handle, ambiguous fragrance, and delicious taste. The columnar mushroom is rich in nutrients, high in protein, contains a variety of essential amino acids, and is rich in B vitamins and mineral elements such as potassium, sodium, calcium, magnesium, iron, and zinc. Agrocybe cylindrica has the effects of clearing heat, calming the liver, improving eyesight, diuresis, and invigorating the spleen. In recent years, the market demand has increased rapidly. The time for artificial domestication and successful cultivation of Camellia oleifera is very short. Although the output of artificial cultivation has increased greatly in recent years, due to its lon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/40A01G18/20
CPCA01G18/40A01G18/20
Inventor 李威李敬廖之锦倪玉善杨绍翠陈家平
Owner 贵州省贵福菌业发展有限公司
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