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Use of 3,6-anhydro-l-galactose for preventing dental caries

一种半乳糖、龋齿的技术，应用在3领域，能够解决天然糖不足等问题

Active Publication Date: 2018-11-23
KOREA UNIV RES & BUSINESS FOUND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Xylitol is a 5-carbon alcohol that exists in nature. It is manufactured and widely used as chewing gum because of its sweetness and refreshing taste similar to sugar, but it has the disadvantage that it can be inhibited only in high concentrations of xylitol Streptococcus mutans growth
[0003] Currently, research on natural materials with antimicrobial activity against Streptococcus mutans is increasingly conducted, and more and more attention is paid to natural sugars as anti-cariogenic sweeteners, but studies have found anti-cariogenic properties similar to xylitol Insufficient natural sugars for action

Method used

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  • Use of 3,6-anhydro-l-galactose for preventing dental caries
  • Use of 3,6-anhydro-l-galactose for preventing dental caries
  • Use of 3,6-anhydro-l-galactose for preventing dental caries

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0108] Agarose pretreatment

[0109] The agarose was dissolved in 3N acetic acid to a concentration of 7% (w / v), and reacted at 130°C for 10 minutes using a microwave digester, and then the reaction product was washed twice with 1.5L of 94% ethanol to remove residual acetic acid and excess Decomposition products. The reaction product was freeze-dried for 24 hours to obtain powdered agar-oligosaccharides.

Embodiment 2

[0110] 3,6-Anhydrous-L-galactose is produced by enzymatic hydrolysis of agar-oligosaccharides

[0111] The acid hydrolysate (agar oligosaccharide) produced in Example 1 was reacted with exo-type β-agarosease (i.e., Aga50D) (refer to Korean Patent Application Publication No. 2010-0040438 published on April 20, 2010) , In order to make it decomposed into D-galactose and 3,6-anhydrous-L-galactose (both monosaccharides), and as a reaction product to generate agarotriose and new agarobiose.

[0112] After the Aga50D reaction is completed, the agarotriose is treated with the enzyme VejABG (enzyme that can decompose the β-1,4 bond at the non-reducing end) (see Korean Patent Application Publication No. 2015-0043040 published on April 22, 2015) , To produce 3,6-anhydrous-L-galactose and new agarbiose.

[0113] In order to produce D-galactose and 3,6-anhydrous-L-galactose (monosaccharides) from New Sepharose, the reaction product of VejABG and the SdNABH enzyme (see Korean Patent Application...

Embodiment 3

[0115] Separation and purification using silica gel chromatography and Bio-Gel P2 chromatography

[0116] Chromatography was performed to separate and purify only 3,6-anhydrous-L-galactose from the reaction product prepared in Example 2. The reaction product is adsorbed on diatomaceous earth to form a sample in powder form, and then subjected to silica gel chromatography (adsorption chromatography). A solvent obtained by mixing chloroform, methanol, and water in a ratio of 78:20:2 (v / v / v) was used as the mobile phase, and the total volume of the solvent as the mobile phase was 4L. The volume of one part is 20 mL, and a sample consisting of a total of 200 parts is analyzed by TLC, thereby collecting the part containing 3,6-anhydrous-L-galactose therein, and removing the organic solvent therefrom using a rotary vacuum concentrator .

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PUM

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Abstract

The present invention relates to a use of 3,6-anhydro-L-galactose for preventing dental caries. More specifically, 3,6-anhydro-L-galactose inhibits the growth of oral microorganisms and exhibits anti-caries activity to inhibit the production of acids caused by the consumption of a carbon source by the oral microorganisms. Thus, 3,6-anhydro-L-galactose can be used in pharmaceuticals, food products,oral hygiene preparations, etc. for preventing, ameliorating or treating oral diseases caused by oral microorganisms, such as dental caries, gingivitis, periodontitis, oral mucosal ulcer, halitosis or xerostomia.

Description

Technical field [0001] The present invention relates to the anti-caries use of 3,6-anhydrous-L-lactose for preventing or treating oral diseases by inhibiting the growth of oral microorganisms and inhibiting acid production. Background technique [0002] Tooth decay is one of the main reasons for people to visit the dentist's oral disease, and as humans use sugar as a sweetener, its incidence is increasing. Streptococcus mutans is the main pathogenic bacteria of dental caries (Loesche WJ et al. (1975) Infect Immun. 11(6):1252-60). Streptococcus mutans breaks down sugar in the oral cavity and secretes glucosyltransferase (GTF) to form insoluble glucans on the surface of teeth, which causes Streptococcus mutans or various other bacteria to attach to the surface of teeth to proliferate thereon. Bacteria on the tooth surface cause dental caries, which is due to the decalcification of the enamel on the tooth surface by organic acids such as lactic acid as a carbohydrate metabolite (Da...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7004A61K8/60A23L33/10A61Q11/00
CPCA61Q11/00A61K31/7004A61K8/60A23L33/125A23V2002/00A61K9/0056A23V2200/312A23V2250/608
Inventor 金京宪崔仁杰尹银珠李雅凛
Owner KOREA UNIV RES & BUSINESS FOUND
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