Method for preparing pravastatin-loaded vascular tissue engineering scaffold material by electrospinning
An electrospinning and vascular tissue technology, applied in the field of fibrous artificial vascular materials, can solve the problems of non-degradable, non-degradable, and only 1 to 7 days, and achieve the effects of easy regulation, simple method, and prevention of recurrence.
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Embodiment 1
[0023] 1. Preparation of scaffold materials for vascular tissue engineering
[0024] (1) Add poly(3-hydroxybutyrate-3-hydroxyhexanoate) into dichloromethane, heat and magnetically stir in a water bath at 30°C for 24 hours, and prepare a polyhydroxyalkanoate solution with a mass concentration of 7%, statically Remove air bubbles after placing;
[0025] (2) Add pravastatin sodium in acetic acid, stir magnetically at 25° C., and make a pravastatin sodium solution with a concentration of 20 mg / mL;
[0026] (3) According to the volume ratio of polyhydroxyalkanoate solution: pravastatin sodium solution=1:1, under the condition of mixing slowly while stirring, the polyhydroxyalkanoate solution and pravastatin sodium solution are mixed uniformly, Obtain an electrospinning solution;
[0027] (4) Put the electrospinning solution obtained in step (3) into an electrospinning device for electrospinning to obtain micro-nano fibers, and dry them in vacuum at 60° C. for 24 hours to obtain t...
Embodiment 2
[0031] 1. Preparation of scaffold materials for vascular tissue engineering
[0032] (1) Add poly(3-hydroxybutyrate-3-hydroxyvalerate) into a mixed solvent of chloroform and DMF, heat and magnetically stir in a water bath at 50°C for 24 hours, and prepare polyhydroxyalkane with a mass concentration of 30%. Ester solution, remove bubbles after leaving standstill; Wherein the mixed volume ratio of chloroform and DMF in the mixed solvent is 8: 2;
[0033] (2) Add pravastatin sodium to isopropanol, stir magnetically at 40° C., and prepare a pravastatin sodium solution with a concentration of 300 mg / mL;
[0034] (3) According to the volume ratio of polyhydroxyalkanoate solution: pravastatin sodium solution=8:1, under the condition of mixing slowly while stirring, the polyhydroxyalkanoate solution and pravastatin sodium solution are mixed uniformly, Obtain an electrospinning solution;
[0035] (4) Put the electrospinning solution obtained in step (3) into an electrospinning device...
Embodiment 3
[0039] 1. Preparation of scaffold materials for vascular tissue engineering
[0040] (1) Add poly(3-hydroxybutyrate-4-hydroxybutyrate) into chloroform, heat and magnetically stir in a water bath at 40°C for 12h, and prepare a polyhydroxyalkanoate solution with a mass concentration of 20%, statically Remove air bubbles after placing;
[0041] (2) Pravastatin sodium is added in the mixed solvent of ethanol and DMF, under 25 ℃ magnetic stirring, be made into the pravastatin sodium solution that concentration is 100mg / mL; Wherein the volume ratio of ethanol and DMF in the said mixed solvent is 1:1;
[0042] (3) According to the volume ratio of polyhydroxyalkanoate solution:pravastatin sodium solution=6:1, under the condition of mixing slowly while stirring, the polyhydroxyalkanoate solution and pravastatin sodium solution are mixed evenly, Obtain an electrospinning solution;
[0043] (4) Put the electrospinning solution obtained in step (3) into an electrospinning device for el...
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