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Tissue engineering scaffold with vascular structure and preparation method thereof

A tissue engineering scaffold and vascular structure technology, applied in the field of biomedicine and tissue engineering materials, can solve the problems of poor material exchange and transport capacity, difficulty in forming a vascular network, difficulty in maintaining cell activity, etc., and achieve high plasticity, mechanical strength and mechanical strength. Excellent performance and the effect of maintaining cell viability

Active Publication Date: 2018-12-07
登腾(上海)医疗器械有限公司
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  • Abstract
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  • Claims
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Problems solved by technology

[0002] At present, 3D printing technology is widely used in the fields of biomedicine and tissue engineering materials. Through fast and efficient personalized preparation, bionic tissues or organs are expected to be applied to the repair of cartilage, skin, blood vessels, nerves, cardiac muscle and other tissues. However, existing Advanced 3D bioprinting technology is difficult to obtain a complex three-dimensional structure, and it is difficult to form a vascular network that provides the nutrients required for tissue production. Therefore, only a very thin layer of tissue can be printed to ensure that the cells inside the material obtain nutrients and the normal discharge of metabolites ; while building a thicker 3D printed tissue engineering scaffold, due to its poor material exchange and transport capacity, it is difficult to obtain sufficient nutrition and metabolic exchange inside, which will cause necrosis and make it difficult to maintain cell activity

Method used

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  • Tissue engineering scaffold with vascular structure and preparation method thereof

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preparation example Construction

[0014] Provide a kind of preparation method of the tissue engineering scaffold with vascular structure in one embodiment of the present invention, comprise the following steps:

[0015] S1. Construct a three-dimensional network model, and print out the support of the three-dimensional network structure through 3D printing;

[0016] S2. Immerse the scaffold printed by S1 in the ethanol mixed solution of calcium salt, and obtain the scaffold with calcium salt on the surface after the ethanol volatilizes;

[0017] S3. filling the modified photocurable polymer in the sacrificial scaffold in step S2, and forming a cured hydrogel scaffold through photocuring;

[0018] S4. Put the cured hydrogel scaffold in water, PBS or cell culture medium to remove the sacrificial scaffold to obtain the tissue engineering scaffold with bionic vascular network.

[0019] The sacrificial stent material used in the preparation of the tissue engineering stent with vascular structure in the present inve...

Embodiment 1

[0023] A preparation method of a tissue engineering scaffold with a vascular structure, comprising the following steps:

[0024] S1. Construct a three-dimensional network model, and print out the support of the three-dimensional network structure through 3D printing:

[0025] Concretely include: building a three-dimensional network model through MIMICS software, pouring a fructose solution with a concentration of 3g / ml into the barrel of the 3D printer, setting the temperature of the syringe to 120°C, the temperature of the receiving plate to 30°C, and the inner diameter of the needle to 0.13 mm, the distance between the needle head and the bracket receiving device is 0.5mm, the distance between the XY axes is 1.5mm, the moving speed of the XY axis platform is 1.5mm / s, and the extrusion rate is 0.01mm 3 / s, the Z-axis step height is 0.5mm, and the size is 20mm×20mm, and the sacrificial fructose support of the three-dimensional network structure with the gap of 1.5mm is obtaine...

Embodiment 2

[0036] A preparation method of a tissue engineering scaffold with a vascular structure, comprising the following steps:

[0037] S1. Construct a three-dimensional network model, and print out the support of the three-dimensional network structure through 3D printing:

[0038] Concretely include: building a three-dimensional network model through MIMICS software, pouring a glucose solution with a concentration of 2g / ml into the barrel of the 3D printer, setting the temperature of the syringe to 150°C, the temperature of the receiving plate to 30°C, and the inner diameter of the needle to 0.6 mm, the distance between the needle head and the bracket receiving device is 0.5mm, the distance between the XY axes is 1.5mm, the moving speed of the XY axis platform is 1.5mm / s, and the extrusion rate is 0.01mm 3 / s, the Z-axis step height is 0.5mm, and a sacrificial glucose scaffold with a three-dimensional network structure with a size of 20mm×20mm and a gap of 1.5mm is obtained;

[00...

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Abstract

The invention discloses a tissue engineering scaffold with a vascular structure and a preparation method thereof. The preparation method comprises the following steps: S1, establishing a three-dimensional stereoscopic network model, and performing 3D printing to obtain a sugar scaffold of a three-dimensional network structure; S2, immersing the sugar scaffold printed in S1 into an ethanol mixed solution of a calcium salt, and obtaining a sugar scaffold loaded with the calcium salt on the surface after ethanol volatilization; S3, filling modified light cured polymers in the sugar scaffold in the S2, and performing light curing to form a cured hydrogel scaffold; and S4, placing the cured hydrogel scaffold in water, PBS or a cell culture medium to remove the sugar scaffold, thereby obtainingthe tissue engineering scaffold with the bionic vascular network. The internal vascular structure and the like have integral connectivity, high precision and excellent mechanical strength and mechanical property, living cells can be directly loaded while the bionic vascular network and the tissue engineering scaffold are molded, and the cell viability is maintained for a long time.

Description

technical field [0001] The invention relates to the technical field of biomedicine and tissue engineering materials, in particular to a tissue engineering scaffold with a vascular structure and a preparation method thereof. Background technique [0002] At present, 3D printing technology is widely used in the fields of biomedicine and tissue engineering materials. Through fast and efficient personalized preparation, bionic tissues or organs are expected to be applied to the repair of cartilage, skin, blood vessels, nerves, cardiac muscle and other tissues. However, existing Advanced 3D bioprinting technology is difficult to obtain complex three-dimensional structures, and it is difficult to form a vascular network that provides nutrients required for tissue production. Therefore, only a very thin layer of tissue can be printed to ensure that the cells inside the material obtain nutrients and the normal discharge of metabolites ; while constructing a thicker 3D printed tissue...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/20A61L27/22A61L27/24A61L27/02A61L27/50A61L27/60
CPCA61L27/025A61L27/20A61L27/222A61L27/227A61L27/24A61L27/50A61L27/60A61L2430/06A61L2430/20C08L5/04C08L5/08C08L89/00C08L1/02C08L5/02
Inventor 杨高洁吴苏州李晓云陈明惠
Owner 登腾(上海)医疗器械有限公司
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