A marine microorganism ys2071 lipase stability protector
A marine microorganism, lipase technology, applied in the direction of enzyme stabilization, biochemical equipment and methods, enzymes, etc., can solve the problems of poor thermal stability, easy inactivation, affecting development and application, etc., to achieve broad application prospects and improve thermal stability. Effect
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Embodiment 1
[0017] Embodiment 1 The impact of alcohols on the stability of marine microorganism YS2071 lipase enzyme activity
[0018] Prepare glycerol, propylene glycol, ethanol, and sorbitol solutions with equal volumes of 1.5, 3, 4.5, 6, 7.5, 9, and 10.5%, respectively, and add marine microorganism YS2071 lipase enzyme powder to the above solutions to make the enzyme liquid final. The concentration was 2 mg / mL, and no reagent was added to the control group. The enzyme solutions of each group were placed in a 40°C incubator for 48 hours, and the remaining enzyme activity of the enzyme solutions of each group was measured after 48 hours.
[0019] Polyols (glycerol, propylene glycol, ethylene glycol, polyol-D), polyethylene glycol (PEG), thickener-B, sodium alginate, etc. can combine with water molecules to reduce the activity of water and make enzymes Proteins are less affected by water molecules, and after adding these substances, the viscosity of the enzyme solution increases, making t...
Embodiment 2
[0020] Embodiment 2 Effects of different sugars on the stability of marine microorganism YS2071 lipase
[0021] Add glucose, α-lactose, sucrose, fructose, xylan, and maltose solutions to equal volumes of 2 mg / mL marine microbial YS2071 lipase solution to prepare equal volumes of 2%, 4%, and 6% respectively , 8%, 10%, 12% glucose, α-lactose, sucrose, fructose, xylan, and maltose solutions, add enzyme powder to the above solutions respectively, so that the final concentration of the enzyme solution is 2mg / mL, and the control group does not add For any reagent, place each group of enzyme solutions in a 40°C incubator for 48 hours, and measure the remaining enzyme activity of each group of enzyme solutions after 48 hours.
[0022] Effects of different concentrations of sugars on the stability of lipase activity of marine microorganism YS2071 as follows: figure 2 , it can be seen that fructose has a certain protective effect on the lipase activity of marine microorganism YS2071, ...
Embodiment 3
[0023] The influence of embodiment 3 preservatives on the activity stability of marine microorganism YS2071 lipase
[0024] Add preservatives such as sodium benzoate and potassium sorbate to an equal volume of 2mg / ml marine microorganism YS2071 lipase enzyme liquid respectively, so that the content of the additive is 0.4%. The control group does not add any additives. Place in an incubator at ℃ for 48 hours, and measure the remaining enzyme activity of each enzyme solution after 48 hours.
[0025] The effect of sodium benzoate on the stability of enzyme activity is as follows: image 3 As shown, the results show that when the sodium benzoate content is 1.6%, the enzyme activity retention rate reaches the highest, which is 65.00%, and the stabilizing effect is good. The effect of potassium sorbate on the stability of enzyme activity is as follows: Figure 4 As shown, when the potassium sorbate content is 0.4%, the enzyme activity retention rate reaches the maximum, which is 6...
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