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Tin-antimony oxide nanoparticles with peroxidase-like characteristic and preparation method and application thereof

An antimony tin oxide, nanoparticle technology, applied in chemical instruments and methods, nanotechnology for materials and surface science, nanotechnology, etc., can solve the problem of large-scale use, decreased HRP activity, and reaction conditions. Advanced problems, to achieve the effect of high yield, simple preparation process and good repeatability

Active Publication Date: 2018-12-14
JIANGSU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Although HRP is widely used as a peroxidase, it is subject to many limitations when used as a biological enzyme. For example, the reaction conditions need to be relatively mild: studies have shown that HRP has begun to inactivate when the pH is lower than 6, and when the temperature is higher than The activity of HRP also began to drop sharply at 40°C
At the same time, in addition to high requirements on reaction conditions, HRP is also relatively expensive as a biological enzyme itself, so it cannot be used on a large scale

Method used

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  • Tin-antimony oxide nanoparticles with peroxidase-like characteristic and preparation method and application thereof
  • Tin-antimony oxide nanoparticles with peroxidase-like characteristic and preparation method and application thereof
  • Tin-antimony oxide nanoparticles with peroxidase-like characteristic and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Weigh 1g of tin particles and 0.44g of antimony trioxide powder into a 100mL three-necked flask, and add 8mL of concentrated sulfuric acid. Then the mixed solution was magnetically stirred and heated at 95° C. for 10 hours. After the heating was completed, the solution was mixed in an ice-water bath, and 32 mL of hydrogen peroxide was slowly added dropwise. After 20 minutes, 24 mL of ultrapure water was added under the condition of magnetic stirring, and stirred for 20 minutes. Finally, the mixed solution was transferred to a 100 mL autoclave, and reacted in an oven at 160° C. for 10 hours. After the reaction, the mixed liquid was centrifuged (rotating speed 5500rpm, 5 minutes), and the precipitate was washed 3 times with ultra-pure water, then 1 time with ethanol, dried, and ground into powder to obtain 35% antimony-doped oxidation Tin-antimony nanopowder, sealed and preserved for future use.

Embodiment 2

[0030] Weigh 1g of tin particles and 0.31g of antimony trioxide powder into a 100mL three-necked flask, and add 8mL of concentrated sulfuric acid. Then the mixed solution was magnetically stirred and heated at 95° C. for 10 hours. After the heating was completed, the solution was mixed in an ice-water bath, and 32 mL of hydrogen peroxide was slowly added dropwise. After 20 minutes, 24 mL of ultrapure water was added under the condition of magnetic stirring, and stirred for 20 minutes. Finally, the mixed solution was transferred to a 100 mL autoclave, and reacted in an oven at 160° C. for 10 hours. After the reaction is over, centrifuge the mixed liquid (5500 rpm for 5 minutes), take the precipitate, wash it with ultrapure water for 3 times, then wash it with ethanol once, dry it, and grind it into powder to obtain tin oxide with 25% antimony doping Antimony nanopowder, sealed for future use.

Embodiment 3

[0032] Weigh 1g of tin particles and 0.18g of antimony trioxide powder into a 100mL three-necked flask, and add 8mL of concentrated sulfuric acid. Then the mixed solution was magnetically stirred and heated at 95° C. for 10 hours. After the heating was completed, the solution was mixed in an ice-water bath, and 32 mL of hydrogen peroxide was slowly added dropwise. After 20 minutes, 24 mL of ultrapure water was added under the condition of magnetic stirring, and stirred for 20 minutes. Finally, the mixed solution was transferred to a 100 mL autoclave, and reacted in an oven at 160° C. for 10 hours. After the reaction, the mixed liquid was centrifuged (rotating speed 5500rpm, 5 minutes), and the precipitate was washed 3 times with ultrapure water, then 1 time with ethanol, dried, and ground into powder to obtain 15% antimony-doped oxidation Tin-antimony nanopowder, sealed and preserved for future use.

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Abstract

The invention discloses tin-antimony oxide nanoparticles with a peroxidase-like characteristic and a preparation method and an application thereof. The tin-antimony oxide nanoparticles are prepared mainly by the steps: mixing and heating tin, antimony trioxide and concentrated sulfuric acid, then successively adding hydrogen peroxide and ultrapure water, carrying out hydrothermal reaction, finally, centrifuging and separating a precipitate, washing, drying and grinding to obtain the tin-antimony oxide nanoparticles containing tin, antimony and oxygen elements. The preparation method has the advantages of sufficient sources of used raw materials, low production cost, simple material preparation process, simple operation and strong repeatability; the obtained tin-antimony oxide nanoparticleshave uniform crystal grain size, can be uniformly dispersed in an aqueous phase in the presence of triethylamine, can catalyze the oxidation of a chromogenic substrate 3,3',5,5'-tetramethylbenzidineby hydrogen peroxide, and have great potential applications in the fields of food testing, pollutant treatment and the like.

Description

technical field [0001] The invention relates to a tin antimony oxide nanoparticle with peroxidase-like properties and a preparation method and application thereof, belonging to the technical field of synthesis of nanometer analysis materials. Background technique [0002] There are many types of catalyst systems, among which enzyme catalyst is a very important part of the catalyst. The selectivity of enzyme catalysts is extremely high, that is, an enzyme can usually only catalyze one or one type of reaction, and can only catalyze the conversion of one or one type of reactant (also known as substrate), including the selectivity in stereochemical structure . Among enzyme catalysts, horseradish peroxidase (HRP) is a common peroxidase and has been widely used in enzyme immunoassays. Among the three substrates in enzyme immunoassays, namely chromogen substrate, fluorescent substrate and chemiluminescent substrate, tetramethylbenzidine (3,3',5,5'-Tetramethylbenzidine, TMB) is us...

Claims

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Application Information

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IPC IPC(8): C09K11/75B82Y30/00B82Y40/00G01N21/64
CPCB82Y30/00B82Y40/00C09K11/751G01N21/643
Inventor 唐盛孙俊李亚拿沈薇祁桐毛威刘凯朱安妮许孟媛许孟婵
Owner JIANGSU UNIV OF SCI & TECH
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