Preparation method of Clostridium butyricum powder

[0025] Example 1 Influence of inlet temperature

[0026] Adjust the inlet temperature of spray drying, and directly spray dry the untreated Clostridium butyricum fermentation broth, and the number of viable bacteria in the original bacterial solution is 5.7×10 8 cfu/mL, the obtained bacterial powder was counted on iron sulfite plates (the plate counting method is also used below), and the plate count was performed again after placing at 40ºC for 10 days. The results are shown in Table 1:

[0027] Table 1 Influence of inlet temperature

[0028]

[0029] Note: The number of viable bacteria 1 is the number of viable bacteria obtained by instant detection of the powder obtained by spray drying; the number of viable bacteria is the number of viable bacteria after being placed at 40ºC for 10 days; the number of viable bacteria 3 is placed at room temperature for 3 months The number of viable bacteria; the mass yield is the quality of the bacteria powder obtained by spraying 100 mL of fermentation stock solution; the viable bacteria yield is the ratio of the number of viable bacteria in the bacteria powder to the number of viable bacteria in the fermentation stock solution; the survival rate is the bacteria powder after storage The ratio of the number of viable bacteria to the number of viable bacteria in the powder immediately after spraying.

[0030] Example 2 Influence of centrifugal pretreatment of bacterial liquid

[0031] Take 100 mL of Clostridium butyricum fermentation broth, centrifuge at 4000 rmp for 10 min, and take the bacterial slurry, then reduce it with an equal volume of sterile water, and add 20% sodium alginate to the treated bacterial suspension; ferment at 100 mL. 20% sodium alginate was directly added to the solution as a control, and the viable count and stability of the spray-dried bacterial powder were compared. The inlet temperature of the sprayer during spray drying was 180ºC.

[0032] Three batches of fermentation broth were tested at the same time, and the number of viable bacteria in batch 1 was 7.2×10 8 cfu/mL, the number of viable bacteria in batch 2 is 5.7×10 8 cfu/mL batch, the number of viable bacteria in batch 3 is 2.7×10 8 cfu/mL. The results are shown in Table 2:

[0033]

[0034] Note: The number of viable bacteria 1 is the number of viable bacteria immediately detected; the number of viable bacteria 2 is the number of viable bacteria after being placed at 40ºC for 10 days; the number of viable bacteria 3 is the number of viable bacteria placed at room temperature for 3 months; the mass yield is 100 mL of fermentation stock solution after The quality of the bacteria powder obtained by spraying; the viable bacteria yield is the ratio of the number of viable bacteria in the bacteria powder to the number of viable bacteria in the fermentation stock solution; the survival rate is the difference between the number of viable bacteria in the powder after storage and the number of viable bacteria in the powder just after spraying. ratio.

[0035] The results showed that after the fermentation broth was treated, the bacterial powder was placed at 40ºC for 10 d and at room temperature for three months, with stable performance and good inter-batch stability. By analyzing the data, it can be found that the number of viable bacteria after being placed at 40ºC for 10 days is not significantly different from the number of viable bacteria placed at room temperature for three months. Therefore, in the later test, only the number of viable bacteria at 40ºC for 10 days can be detected to characterize the bacteria treated by the corresponding method. Powder stability, while shortening the test period.

[0036] Example 3 Influence of centrifugation conditions

[0037] Take 10 mL of fermentation broth respectively, centrifuge under different conditions in Table 3, discard the supernatant, add sterile water to 10 mL, and detect the viable bacteria content in the bacterial suspension after mixing. The results are shown in Table 3. :

[0038]

[0039] The results showed that when the rotation speed was 4000 rpm and the centrifugation time was 10 min, the residual amount of viable bacteria in the bacterial sludge was the highest.