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Preparation method of Clostridium butyricum powder

A technology of Clostridium butyricum and bacteria powder is applied in the directions of microorganism-based methods, biochemical equipment and methods, bacteria, etc., to achieve the effects of good stability, strong process controllability and low production cost.

Active Publication Date: 2018-12-18
HENAN JINBAIHE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the spray drying method is a relatively commonly used process, how to provide a preparation process for a bacterial powder with a high number of viable bacteria and good stability for different active ingredients, such as Clostridium butyricum liquid products with poor stability, still needs to be solved The problem

Method used

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  • Preparation method of Clostridium butyricum powder
  • Preparation method of Clostridium butyricum powder
  • Preparation method of Clostridium butyricum powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1 Influence of inlet temperature

[0026] Adjust the inlet temperature of the spray drying, spray and dry the untreated Clostridium butyricum fermentation broth directly, the number of live bacteria in the original bacterial liquid is 5.7×10 8 cfu / mL, the ferric sulfite plate counting method was used for the obtained bacterial powder (the plate counting method is also used in the following), and the plate counting was performed again after 10 days at 40°C. The results are shown in Table 1:

[0027] Table 1 Effect of inlet temperature

[0028]

[0029] Note: The number of viable bacteria 1 is the number of viable bacteria obtained by the instant detection of the bacteria powder obtained by spray drying, that is, the number of viable bacteria; the number of viable bacteria 2 is the number of viable bacteria after being placed at 40ºC for 10 days; the number of viable bacteria 3 is the number of viable bacteria after being placed at room temperature for 3 m...

Embodiment 2

[0030] Embodiment 2 Bacterial liquid carries out the impact of centrifugation pretreatment

[0031] Take 100 mL of Clostridium butyricum fermentation broth, centrifuge at 4000 rpm for 10 min, take the sludge, and then reduce it with an equal volume of sterile water, add 20% sodium alginate to the treated bacterial suspension; ferment with 100 mL 20% sodium alginate was directly added to the solution as a control, and the number of viable bacteria and stability of the spray-dried bacteria powder were compared. The inlet temperature of the nebulizer during spray drying was 180ºC.

[0032] Three batches of fermentation broth were tested at the same time, and the number of viable bacteria in batch 1 was 7.2×10 8 cfu / mL, the number of viable bacteria in batch 2 is 5.7×10 8 cfu / mL batch, the number of viable bacteria in batch 3 is 2.7×10 8 cfu / mL. The results are shown in Table 2:

[0033]

[0034] Note: The number of viable bacteria 1 is the number of viable bacteria imm...

Embodiment 3

[0036] Embodiment 3 The influence of centrifugation condition

[0037] Take 10 mL of fermentation broth, centrifuge under the different conditions in Table 3, discard the supernatant, add sterile water to 10 mL, and measure the number of viable bacteria in the bacterial suspension after mixing. The results are shown in Table 3 :

[0038]

[0039] The results showed that when the rotational speed was 4000 rpm and the centrifugation time was 10 min, the residual amount of viable bacteria in the sludge was the highest.

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Abstract

The invention belongs to the technical field of fungus powder preparation, and especially relates to a preparation method of Clostridium butyricum powder. A Clostridium butyricum fermentation solutionundergoes spray drying, and the inlet temperature of spray drying is 150-180 DEG C. The preparation method of the Clostridium butyricum powder has the advantages of low production cost and strong process controllability, and the obtained Clostridium butyricum powder contains a large number of live bacteria, has a good storage stability at room temperature, and has great values in the promotion ofthe Clostridium butyricum market.

Description

technical field [0001] The invention belongs to the technical field of bacterial powder preparation, in particular to a preparation method of Clostridium butyricum bacterial powder. Background technique [0002] Clostridium butyricum (Clostridium butyricum) is also known as butyric acid bacteria, Clostridium butyricum. Clostridium butyricum is a gram-positive bacterium, obligate anaerobic, straight rod-shaped or slightly curved, with endogenous spores; the colonies are irregular round, white or milky white, with a sour odor; the suitable growth temperature is 25ºC ~37ºC, the suitable growth pH is 4.0~9.8; can utilize glucose, sucrose, fructose, maltose, starch, cellulose and other carbon sources, and the fermentation product is mainly butyric acid. Clostridium butyricum is a kind of normal flora in the intestinal tract of humans and animals. It has probiotic properties such as regulating the intestinal microecological balance, enhancing immunity, and producing probiotics. ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/04C12R1/145
CPCC12N1/04C12N1/20
Inventor 李永红李克克冯利菲朱广跃李光华
Owner HENAN JINBAIHE BIOTECH CO LTD
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