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Copeptin immunochromatographic detection reagent card and preparation method thereof

An immunochromatographic detection and reagent card technology, which is applied in the field of biomedicine, can solve the problems of complex detection operation of the kit, failure to meet the detection requirements, time-consuming results, etc., and achieve the effect of rapid diagnosis, high sensitivity and simple operation

Inactive Publication Date: 2018-12-21
广州华澳生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the detection kits currently on the market are complex, time-consuming, and the results have certain deviations, which cannot meet the detection needs

Method used

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  • Copeptin immunochromatographic detection reagent card and preparation method thereof
  • Copeptin immunochromatographic detection reagent card and preparation method thereof
  • Copeptin immunochromatographic detection reagent card and preparation method thereof

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preparation example Construction

[0042] A method for preparing a copeptin immunochromatographic detection reagent card, comprising a method for preparing a sample pad, an anti- copeptin antibody tracer label pad and a coating film. The anti-copeptin antibody tracer labeling pad includes anti-copeptin antibody quantum dots, rare earth complexes, anti-copeptin antibody polystyrene nano fluorescent microspheres, colored polystyrene microspheres and anti-copeptin Antibody colloidal gold. The preparation steps of the anti-copeptin antibody quantum dots and rare earth complex label pads are:

[0043] ① Dissolve water-soluble carboxyl quantum dots and lanthanide rare earth complexes with labeling buffer;

[0044] ② Add 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloric acid solution to the quantum dot and lanthanide rare earth complex solution, react at room temperature for 1-2 hours, and remove unreacted Add labeling buffer after chemicals;

[0045] ③ Add anti-peptin antibody to the above-mentioned activ...

Embodiment 1

[0062] Example 1, preparation method of copeptin immunochromatographic detection reagent card (quantum dots, rare earth complex labeling):

[0063] 1) The preparation method of the sample pad is:

[0064] Use 0.01M phosphoric acid containing 0.5~5wt% BSA, 0.5~5wt% polyvinylpyrrolidone, 0.5~5wt% polyethylene glycol, 0.1~5wt% Tween-20, 1~5wt% Triton-100 for the sample pad After soaking in salt buffer solution (0.01 M disodium hydrogen phosphate: 0.01M sodium dihydrogen phosphate = 81:19 (V: V)) (pH 7.4), dry at 37°C or in an environment with a relative humidity of ≤35% , cropped for use.

[0065] The sample pad buffer is preferably 0.5wt% BSA, 0.5wt% polyvinylpyrrolidone, 0.5wt% polyethylene glycol, 0.25wt% Tween-20 and 1wt% Triton-100 dissolved in 0.01M phosphate buffer.

[0066] 2) The preparation method of anti-copeptin antibody quantum dots or rare earth complex labeled pads is:

[0067] ① Dissolve and dilute the water-soluble carboxyl CdSe / ZnS or Eu(TTA) 3Phen to 1 μM wi...

Embodiment 2

[0087] Example 2, preparation method of copeptin immunochromatographic detection reagent card (labeled with fluorescent nanospheres or colored nanospheres):

[0088] 1) Preparation of anti-copeptin antibody polystyrene nanofluorescent microspheres or colored polystyrene microspheres labeling pad:

[0089] ① Use 0.1M MES buffer solution with pH 6.0 (with 0.1M MES aqueous solution, adjust the pH with 1 M NaOH solution) to mix carboxypolystyrene nano fluorescent microspheres (particle size 190nm) or colored polystyrene microspheres (particle size 200nm) ) to a solid content of about 0.2%;

[0090] ② Take 1mL polystyrene nanofluorescent microspheres or colored polystyrene microspheres with a solid content of 0.2%, add 50μL EDC solution (10mg / mL) and 50μL sulfo-NHS solution (50mg / mL), activate for 10min, 16500r / min Centrifuge for 30 minutes, remove the supernatant, add 240 μg anti-copeptin antibody (primary antibody), and mix the coupling reaction for 1 hour;

[0091] ③ Add 650 μ...

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Abstract

The invention discloses a copeptin immunochromatographic detection reagent card and a preparation method thereof and aims to provide a copeptin detection method which is quick and convenient to use, high in sensitivity and reliable in detection result. The detection reagent card comprises a box body, wherein the box body is provided with a base plate and a box cover, the box cover is provided witha sample feeding hole and a result detection window, detection test paper is arranged in the box body, the base plate is arranged below the detection test paper, the detection test paper is providedwith a detection area, the detection area is provided with a sample pad, an anti-copeptin antibody tracer marking pad, a coating film and a water absorption pad, the coating film is provided with a copeptin antibody detection area T-line and a quality control area C-line, and the detection area T-line and the quality control area C-line are arranged in parallel. The reagent box and the method havethe advantages that sensitivity is high, specificity is high, fastness and convenience are achieved, and an analysis object can be measured quantitatively or qualitatively.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a detection reagent card for measuring copeptin content in blood (serum, plasma, whole blood) and a preparation method thereof. Background technique [0002] The hypothalamus-pituitary-adrenal axis, also known as the "stress axis," is activated every time a stroke occurs. During the pathogenesis, arginine vasopressin (AVP, also known as antidiuretic hormone, vasopressin, etc.) secreted by the posterior pituitary gland plays an important role. However, arginine vasopressin (AVP) is secreted and released in pulses, has an extremely unstable structure, a short half-life, and is easily cleared, making it difficult to measure accurately in vitro. [0003] Copeptin (copeptin), as the carbon-terminal partial peptide of pro-arginine vasopressin, is not only produced at an equimolar concentration with AVP, but also has good stability and is easy to detect. Therefore, it can be used...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N33/58G01N33/533G01N33/532G01N33/531
CPCG01N33/531G01N33/532G01N33/533G01N33/54306G01N33/582G01N33/585G01N33/588G01N2333/575
Inventor 于龙波于永涛黎权刘日俊
Owner 广州华澳生物科技有限公司
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