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Fluorescence sensor and its preparation and application

A fluorescent sensor and fluorescent molecule technology, applied in the field of biosensors, can solve the problems of low sensitivity and low specificity, and achieve the effect of good fluorescence recovery rate

Active Publication Date: 2020-12-15
XINXIANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, this type of fluorescent material has defects of low specificity and low sensitivity.

Method used

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  • Fluorescence sensor and its preparation and application
  • Fluorescence sensor and its preparation and application
  • Fluorescence sensor and its preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] This embodiment provides a fluorescent sensor and a preparation method thereof. The preparation method comprises the following steps:

[0039] (1) The graphene oxide was prepared by the Hummer method, and the graphene oxide powder was added to deionized water at a concentration of 1 mg / mL, and ultrasonically dispersed for 3 hours to obtain a uniform graphene oxide water dispersion system;

[0040] (2) Take 100mL graphene oxide water dispersion system, add 20mL crown ether, 3mL ammonia water and 200μL 80% (mass fraction) hydrazine hydrate, stir for 10 minutes and place it at 60°C for 3.5 hours; dialyze after the reaction to obtain the reduced A solution of graphene oxide-crown ether complex;

[0041] (3) Add rhodamine 6G with a final concentration of 1.5 μmol / L to the solution of the prepared reduced graphene oxide-crown ether complex, and place it in the dark at room temperature for 1 hour to carry out inclusion reaction to obtain the reduced A solution of graphene ox...

Embodiment 2

[0044] The present embodiment provides the application of a kind of fluorescence detector in the detection of magnesium ion (derived from magnesium acetate), comprising:

[0045] (1) According to the preparation method described in Example 1, a reduced graphene oxide-crown ether complex solution with a concentration of 0.3333 mg / mL was prepared.

[0046](2) A: Take the above-mentioned reduced graphene oxide-crown ether complex solution, add rhodamine 6G solution respectively, wherein, adjust the final concentration of the reduced graphene oxide-crown ether complex to 0.005mg / mL, rhodamine 6G The final concentrations were 0.25μmol / L, 0.5μmol / L, 0.75μmol / L, 1.0μmol / L, 1.25μmol / L, 1.5μmol / L, 1.75μmol / L, 2.0μmol / L, 2.25μmol / L, 2.5 μmol / L, placed at room temperature in the dark for 1 hour to carry out inclusion reaction, and 10 solutions of reduced graphene oxide-crown ether-rhodamine 6G complexes with different concentrations were obtained.

[0047] The solution of above-mentione...

Embodiment 3

[0052] The present embodiment provides the application of a kind of fluorescence detector in the detection of magnesium ion (derived from magnesium chloride), including:

[0053] (1) According to the preparation method described in Example 1, a reduced graphene oxide-crown ether complex solution of 0.3333 mg / mL was prepared.

[0054] (2) Get above-mentioned reduced graphene oxide-crown ether complex solution, add a certain amount of rhodamine 6G solution respectively, make the final concentration of reduced graphene oxide-crown ether complex be 0.005mg / mL, rhodamine 6G final concentration is 1.5 μmol / L, placed in the dark at room temperature for 1 hour to carry out the inclusion reaction, and obtained the reduced graphene oxide-crown ether-rhodamine 6G complex solution. Under excitation wavelength 480nm, detection wavelength 551nm, measure its fluorescence intensity, record as I 0 . The reduced graphene oxide-crown ether-rhodamine 6G complex solution was equally divided into...

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Abstract

The invention provides a fluorescent sensor, and a preparation method and an application thereof. The fluorescence sensor is a reduced graphene oxide-crown ether-fluorescent molecule complex; the crown ether is modified on the surface of the reduced graphene oxide through hydrogen bonds; and the fluorescent molecule is included in the crown ether. The fluorescent sensor provided by the invention is the reduced graphene oxide-crown ether-fluorescent molecule complex, especially a complex formed by matching a proper type of the crown ether with a proper type of the fluorescent molecule. A cavityof the crown ether in the complex is very matched in size with the fluorescent molecule included in the crown ether or a magnesium ion entering the cavity in a later stage; the bonding force of the crown ether and the fluorescent molecule is moderate; when the fluorescent sensor is used for detecting the magnesium ion, the fluorescent molecule can be replaced very quickly, easily and accurately;and the fluorescent sensor has very high specificity and sensitivity.

Description

technical field [0001] The invention belongs to the technical field of biosensors, and in particular relates to a fluorescent sensor and its preparation and application. Background technique [0002] Magnesium ion is the main cation in human cells. It is concentrated in the mitochondria and ranks third in the extracellular fluid after sodium and calcium. It is an essential substance for the basic biochemical reactions of various cells in the body. Magnesium ions affect a variety of biological functions of cells: affect the transport of potassium ions and calcium ions, regulate the transmission of signals, participate in energy metabolism, protein and nucleic acid synthesis; can complex negatively charged groups, especially phosphate in nucleotides Magnesium is used to maintain the structure and function of substances; the activation and inhibition of catalytic enzymes and the regulation of cell cycle, cell proliferation and cell differentiation; magnesium is also involved in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64C09K11/02
CPCC09K11/025G01N21/6428G01N2021/6432
Inventor 刘巍岳悦于凯利张强刘宇飞
Owner XINXIANG MEDICAL UNIV
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