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A kind of potato endophyte and its application

A potato and endophyte technology, applied in the field of microorganisms, can solve problems such as the biological control of solanine that has not yet been seen, and achieve the effect of improving food safety

Active Publication Date: 2021-10-22
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research on potato endophytes is mainly focused on disease resistance and growth promotion, and there is no research on the biological control of solanine by potato endophytes.

Method used

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  • A kind of potato endophyte and its application
  • A kind of potato endophyte and its application
  • A kind of potato endophyte and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] This example is used to illustrate the isolation, purification and identification of potato endophytes that can inhibit the increase of total solanine content in potatoes.

[0042] 1. Isolation and purification of potato endophytes

[0043] A. Material: The potato variety is Holland 15;

[0044] B. Disinfection: Potato tubers are disinfected with ultrapure water for 15 minutes, soaked in 0.01% Tween-20 for 1 minute, soaked in 3% sodium chlorate for 7 minutes, soaked in 2.5% sodium thiosulfate for 9 minutes, soaked in 75% alcohol for 3 minutes, and washed 5 times in distilled water , wherein 200 μL of the third washing water was used to coat the ISP2 plate, and then soaked in 10% sodium bicarbonate for 10 min;

[0045] C, separation: after cutting the potato tubers sterilized on the surface, get 25g samples respectively, grind them with a sterilized mortar, then transfer them to conical flasks, add 225mL of normal saline to get 10 -1 , fully oscillate. Transfer 1 mL t...

Embodiment 2

[0068] This example is used to illustrate the optimization of the most suitable fermentation conditions for the potato endophyte described in the present invention. Specifically: a single factor optimization test was carried out on the carbon source, nitrogen source, culture temperature, culture time, initial pH value, etc. of the bacterial strain isolated in Example 1 during the fermentation process, and the experimental design was as follows:

[0069] 1. The material used is Holland No. 15 potato. Whole potato tubers with uniform shape and good condition are randomly selected to optimize the fermentation conditions. The single factor design is as follows:

[0070] Carbon sources: glucose, fructose, lactose, sucrose, maltose, starch, mannitol.

[0071] Nitrogen sources: peptone, beef extract, yeast extract, urea, potassium nitrate and ammonium sulfate.

[0072] Culture time: 20h, 40h, 60h, 80h and 100h.

[0073] Culture temperature: 15°C, 20°C, 25°C, 28°C and 30°C.

[0074...

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Abstract

The invention relates to the technical field of microorganisms, and specifically discloses a potato endophyte and its application. The potato endophyte is Bacillus megaterium of the genus Bacillus, which was preserved in the China General Microorganism Culture Collection Management Center on April 3, 2018, address: No. 1 Beichen West Road, Chaoyang District, Beijing No. 3, zip code: 100101, and the deposit number is CGMCC NO.15554. The invention achieves the effect of reducing the content of solanine in the potato by soaking the potato tubers in the fermented liquid of the bacterial strain. The potato endophytic bacteria provided by the present invention can significantly inhibit the increase of solanine content in potato tubers during storage. By inoculating the strains in potato tubers, α-solanine and α-chaconine can be inhibited, and the potato can be improved. food safety. The invention further clarifies the optimum culture formula and fermentation conditions for preparing the potato endophyte fermentation culture liquid, and provides available strains and theoretical basis for further developing and developing potato biocontrol bacteria agents.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a potato endophyte and its application. Background technique [0002] Potato is the fourth largest food crop after wheat, rice and corn in the world. It is a worldwide economic crop. It is widely distributed, easy to cultivate, suitable for food and feed, suitable for various raw materials, and rich in nutrition. Both dishes. [0003] Affected by changes in arable land area, water resources, climate change and other factors, it is more difficult to continue to increase the production of traditional food crops such as wheat, rice, and corn. Potato has the advantages of wide adaptability, cold resistance, drought resistance, and barrenness tolerance, and has great potential for expansion of planting area. The ecological conditions in most parts of China can meet the requirements of potato cultivation and production. Potato production potential is far superior to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01P21/00C12R1/11
CPCA01N63/10C12N1/20C12N1/205C12R2001/11
Inventor 刘佳萌金诺范蓓李敏敏孙玉凤郑旭
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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