A kind of dexamethasone slow-release system and a kind of osteogenesis differentiation induction liquid and application
A technology of dexamethasone and osteogenic differentiation, which is applied in the field of nanomedicine and regenerative medicine, and can solve problems such as osteogenic differentiation that have not yet been retrieved
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Embodiment 1
[0034] Example 1: Preparation of Cyclodextrin / dexamethasine Complex (CD / DEX)
[0035] It is weighted 1.1135 g of beta-Cd (1 mmol) in 20 ml of deionized water, heated to 80 ° C in a stirred state, weighing 0.382 g of dexamethasone (1 mmol) in 10 ml of ethanol, then slowly added to heat In the β-CD solution, stirring was continued for 11.0 hours, stop heating, and stirring at room temperature for 24.0 h, and the refrigerator (4 ° C) was placed overnight, filtered, and a small amount of acetone multiple times. The resulting precipitate was frozen to give the coated product.
[0036] From figure 1 It can be seen that these absorption peaks are obvious in the physical mixture, but 1664cm in the cladding -1 Place Feature peak disappeared, 1621cm -1 C = C telescopic vibration disappears, 1299cm -1 The coupling between OH bending vibration and C-O telescopic vibration is disappeared. This makes it possible to determine that the DEX is enclosed in the β-CD cavity.
Embodiment 2
[0037] Example 2: Preparation of β-cyclodextrin / goldenene-dexamethasone adherate (CD / Ad-DEX)
[0038] Diamamic acid (AD-COOH) (0.1378 g, 0.76 mmol) was dissolved in two in the second of the 2- (3-dimethylaminopropyl) -3-ethyl carbon diimide hydrochloride (0.1465 g, 0.76 mmol) In chloromethane (7 mL), 4-dimethylaminopyridine (0.0156 g, 0.13 mmol) and dexamethamol (0.1000 g, 0.25 mmol) were dissolved in dichloromethane (7 ml) at the same time under expanded light. The mixture was stirred for 15 min, and then the mixture was mixed overnight. The final reaction liquid was separated with silica gel, and the expander was mixed with a metallool of 1 to 30 with a mixed solution of dichloromethane, and the position of Ad-DEX was determined by fluorescence, and then methanol and The mixed solution of the dichloromethane mixed solution was separated. After the AD-DEx was separated, the solvent was rotated to dry, and the resulting white product was ad-den. The AD-DEX powder was dissolved...
Embodiment 3
[0040] Example 3: Preparation of induced induction of bone differentiation efficiency of stem cells according to the present invention
[0041] In the commercially available 500ml low sugar DMEM medium, the volume is added to 10% fetal bovine serum, 10 mmol / Lβ-glycyl phosphate, 50 mg / L ascorbic acid, dexamethasone final concentration of 10 -7 Mol / L adhesion, i.e., new induced fluids that increase the bone differentiation efficiency of stem cells.
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