Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Construction method and application of Guilingji UPLC fingerprint

A fingerprint and construction method technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effect of convenient operation, guaranteed curative effect, and improved product consistency

Active Publication Date: 2019-01-04
山西广誉远国药有限公司
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no literature report on the research on the HPLC fingerprint of Guilingji. In order to ensure the safety and effectiveness of medication for patients, the present invention establishes a fingerprint detection method suitable for the quality control of Guilingji.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Construction method and application of Guilingji UPLC fingerprint
  • Construction method and application of Guilingji UPLC fingerprint
  • Construction method and application of Guilingji UPLC fingerprint

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: Construction of Guilingji UPLC fingerprint

[0031] 1.1 Instrument

[0032] Waters Acquity UPLC H-Class ultra-high performance liquid chromatography system, Empower workstation with quaternary gradient pump, autosampler, PDA detector (Waters company); ultrasonic cleaner (KQ2200DB numerical control ultrasonic cleaner, Kunshan Ultrasonic Instrument Co., Ltd. Company); balance (CPA225D electronic analytical balance, Sartorius Scientific Instrument Co., Ltd.); centrifuge (SC-3160 low-speed centrifuge, Anhui Zhongke Zhongjia Scientific Instrument Co., Ltd.); water bath (HH-ZK4 type, Gongyi Yuhua Instrument Co., Ltd.); Milli-Q Integral Water Purification System (Millipore, USA).

[0033] 1.2 Reagents and Reagents

[0034] 样品:龟龄集(山西广誉远国药有限公司),批号为20130201、20130302、20141112、20150101、20150507、20151212、103160203、103160407、103160508、103160917、103161018、103170305、103170408、103170510、103170714。

[0035] Reagents: ultrapure water; methanol and acetonitrile are chromat...

Embodiment 2

[0052] Example 2: Research on the Methodology of Guiling Collection Fingerprint

[0053] 2.1 Precision test

[0054] Accurately weigh the same batch of Guilingji, prepare the test solution according to the above-mentioned experimental method, continuously inject 6 times according to the above-mentioned chromatographic conditions, and record the chromatogram. The chromatogram obtained by the continuous injection of the same test sample for 6 times is carried out for similarity analysis, and the result shows that its similarity is 1, and the relative retention time of 19 common peaks is investigated, and the RSD of the relative retention time of each chromatographic peak<0.13%, It shows that the precision of the instrument is good.

[0055] 2.2 Repeatability test

[0056] A total of 6 samples of the same batch of Guilingji were weighed, and 6 samples of the test solution were prepared in parallel according to the above-mentioned experimental method, measured according to the a...

Embodiment 3

[0059] Embodiment 3 Guiling set comparison feature map and similarity evaluation

[0060] A total of 15 batches of different batches of Guilingji were taken and analyzed respectively according to the established fingerprint analysis method of Guilingji to obtain the UPLC fingerprint overlay of 15 batches of medicinal materials (such as image 3 shown). Based on the chromatograms of the above-mentioned 15 batches of samples, use the "Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System" (2008 edition) recommended by the Pharmacopoeia Committee to perform data processing to obtain a common pattern characteristic map, which is the Guiling set control characteristic map R (like figure 1 shown). The retention times of the main chromatographic peaks of the 15 batches of samples were basically the same, and the 19 peaks with larger peak areas and better separation under the detection conditions were determined to be their common chromatographic peaks. Calculat...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Boiling pointaaaaaaaaaa
Login to View More

Abstract

The invention discloses a construction method and application of a Guilingji UPLC fingerprint and belongs to the technical field of traditional Chinese medicine. Psoralen, neobavaisoflavone and oleanolic acid are selected as reference samples, and 2-5 microliters of test sample solution and 2-5 microliters of reference sample solution are precisely extracted and separately injected into an ultra-high performance liquid chromatograph for analysis separately; according to chromatographic conditions, a chromatographic column adopts octadecylsilane bonded silica as filler, the column temperature is 25-35 DEG C, the flow rate is 0.1-0.3 mL / min, the detection wavelength is 203 nm, a mobile phase adopts acetonitrile and water gradient elution, and the Guilingji UPLC fingerprint is obtained. According to the Guilingji UPLC fingerprint established by means of the method, the material information of Guilingji can be comprehensively reflected, the precision degree is high, the reproducibility isgreat, and the quality of the Guilingji can be accurately and effectively evaluated and controlled.

Description

technical field [0001] The invention relates to a method for constructing fingerprints of traditional Chinese medicines, in particular to a method for constructing UPLC fingerprints of Guilingji and its application in quality monitoring of Guilingji. Background technique [0002] Guilingji consists of red ginseng, velvet antler, hippocampus, medlar, clove, pangolin, finch brain, achyranthes bidentata, Cynomorium cynomorium, Rehmannia glutinosa, psoralen, dodder, Eucommia ulmoides, stone swallow, cistanche, licorice, asparagus, epimedium , Daqing salt, amomum and other 28 kinds of medicinal materials are processed and refined into a large compound prescription of traditional Chinese medicine (Pharmacopoeia of the People's Republic of China, 2015 edition, page 985). Its formula is huge and its ingredients are complex. In order to maintain the stable efficacy of different batches of this large compound Chinese medicine, it is necessary to formulate strict enterprise internal co...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N30/02G01N30/86
CPCG01N30/02G01N30/8686
Inventor 王佩义郝晋琪秦雪梅高晓霞史静超
Owner 山西广誉远国药有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com