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Method for efficiently separating lignin based on dilute acid preimpregnation

A lignin and pre-impregnation technology, which is applied in the field of biomass separation and conversion, can solve the problems of low yield, long-term mechanical action, fracture, etc., and achieve the effects of high yield, pollution reduction and cost reduction

Active Publication Date: 2019-01-08
BEIJING FORESTRY UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The lignin obtained by this method is considered to be the lignin sample that best represents the original lignin structure, but its disadvantages are low yield and high sugar content
Generally, reducing the crystallinity of cellulose requires long-term mechanical action and the effect is not obvious
In recent years, mechanochemical pretreatment has been used to prepare water-soluble oligosaccharides. After impregnation with mineral liquid acid, high-temperature hydrolysis is carried out to realize the sugar conversion of cellulose and hemicellulose, and finally the remaining lignin components can be obtained. However, this Process that breaks β-O-4 bonds in lignin and model compounds
In addition, the acid pickling stage of the process uses H 2 SO 4 and diethyl ether, due to the pollution of the environment and the high cost of the two reagents, the method is limited to a certain extent in the subsequent industrial application

Method used

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  • Method for efficiently separating lignin based on dilute acid preimpregnation
  • Method for efficiently separating lignin based on dilute acid preimpregnation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Raw material pretreatment stage: crush the eucalyptus wood raw material to 20-100 mesh, and dry it.

[0027] (2) Pre-impregnation-ball milling stage: mix eucalyptus raw material with 5% dilute acetic acid solution at a ratio of 1g: 4ml, stand for impregnation at room temperature for 12h (overnight), freeze-dry it after impregnation, and then freeze-dry The eucalyptus raw material was ball milled (450-500rpm) for 2 hours to obtain the ball milled eucalyptus raw material.

[0028] (3) Enzymatic hydrolysis of treated eucalyptus raw materials: add sodium acetate buffer to the milled eucalyptus raw materials, adjust the pH to 4.8 with acetic acid, then add 10FPU / g cellulose compound enzyme, and enzymatically hydrolyze at 50°C 48h. After the enzymatic hydrolysis, the mixture was centrifuged to remove carbohydrates to obtain an enzymatic hydrolysis residue.

[0029] (4) The enzymolysis residue was repeatedly washed with hot acid water (pH=2) to remove polysaccharides and...

Embodiment 2

[0031] (1) Raw material pretreatment stage: crush the eucalyptus wood raw material to 20-100 mesh and dry it.

[0032] (2) Pre-impregnation-ball milling treatment stage: mix eucalyptus raw material with 2.5% dilute acetic acid solution in a ratio of 1g:3ml, stand for impregnation at room temperature for 12h (overnight), freeze-dry it after impregnation, and then freeze-dry The eucalyptus raw material was ball milled (450-500rpm) for 2 hours to obtain the ball milled eucalyptus raw material.

[0033] (3) Enzymolysis of treated raw materials: Add sodium acetate buffer to ball-milled eucalyptus raw materials, adjust pH to 4.8 with acetic acid, then add 10 FPU / g cellulose compound enzyme, and enzymolyze at 50°C for 48 hours. After the enzymatic hydrolysis, the mixture was centrifuged to remove carbohydrates to obtain an enzymatic hydrolysis residue.

[0034] (4) The enzymolysis residue was repeatedly washed with hot acid water (pH=2) to remove polysaccharides and monosaccharides,...

Embodiment 3

[0036] (1) Raw material pretreatment stage: crush the eucalyptus wood raw material to 20-100 mesh, and dry it.

[0037] (2) Pre-impregnation-ball milling treatment stage: mix eucalyptus raw material with 1% dilute acetic acid solution at a ratio of 1g:5ml, stand for impregnation at room temperature for 12h (overnight), freeze-dry it after impregnation, and then freeze-dry The eucalyptus raw material was ball milled (450-500rpm) for 2 hours to obtain the ball milled eucalyptus raw material.

[0038] (3) Enzymolysis of treated raw materials: Add sodium acetate buffer to ball-milled eucalyptus raw materials, adjust pH to 4.8 with acetic acid, then add 10 FPU / g cellulose compound enzyme, and enzymolyze at 50°C for 48 hours. After the enzymatic hydrolysis, the mixture was centrifuged to remove carbohydrates to obtain an enzymatic hydrolysis residue.

[0039] (4) The enzymolysis residue was repeatedly washed with hot acid water (pH=2) to remove polysaccharides and monosaccharides, an...

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Abstract

The invention discloses a method for efficiently separating lignin based on dilute acid preimpregnation. The method comprises the following steps: a, removing impurities from a biomass raw material, crushing, and drying; b, impregnating the biomass raw material with an aqueous dilute acid solution, then lyophilizing, and then performing ball-milling and crushing to obtain ball-milled biomass; andc, performing hydrolytic treatment on the ball-milled biomass obtained in the step b by using composite cellulose to remove carbohydrates from the biomass and obtain lignin in a form of residue, and repeatedly washing the residue to obtain a high-purity enzymolysis residue, namely the lignin. The invention provides an environmentally-friendly and high-efficiency lignin separating method; the environmentally-friendly and high-efficiency lignin separating method can be used for preparing high-purity and structurally intact lignin and is directly applied to structural characterization; a novel method is provided for interpreting the original lignin structure in the biomass, and a theoretical basis and a technical support are also provided for forest tree breeding and further high-value application of the lignin.

Description

technical field [0001] The invention relates to the field of biomass separation and conversion, in particular to a method for efficiently separating lignin based on dilute acid pre-dipping. Background technique [0002] Due to the massive emission of carbon dioxide and the irreversible consumption of fossil fuels, renewable fuels and biomass-based platform chemicals have attracted widespread attention and gradually occupied a dominant position. Biomass can provide the largest reserves, renewable, high value-added carbon-based materials. In the current biorefinery industry, the comprehensive utilization of lignocellulosic biomass can not only substantially reduce carbon emissions and costs, but also promote the conversion of biomass into high value-added commercial products, which also makes lignocellulose a fossil fuel Economically reasonable alternatives. Lignocellulose is mainly composed of cellulose, hemicellulose and lignin, which are intricately intertwined through va...

Claims

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Application Information

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IPC IPC(8): C08H7/00
CPCC08H6/00
Inventor 孙润仓文甲龙王汉敏袁同琦
Owner BEIJING FORESTRY UNIVERSITY
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