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Method for improving purity of microbial flocculant for sewage treatment

A microbial flocculant and sewage treatment technology, applied in biological water/sewage treatment, water/sludge/sewage treatment, chemical instruments and methods, etc., can solve the constraints of microbial flocculant application, complicated purification process, and product recovery rate Low-level problems, achieving obvious purification effects, overcoming complicated process steps, and high purity

Inactive Publication Date: 2019-01-11
CHENDU NEW KELI CHEM SCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] It can be seen that the microbial flocculant used for sewage treatment in the prior art has the disadvantages of poor stability and low purity, while the traditional purification process is complicated and time-consuming, the product is variable in the extraction process, and the product recovery rate is low, which restricts the microbial recovery. Application of flocculant in sewage treatment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Centrifuge the fermentation liquid of microbial cells at a speed of 2800r / min for 16 minutes, remove most of the cells, and take the supernatant for use; The membrane has stepped pores, the average pore diameter is 20 nm, and the tube pore diameter distribution from the inside to the outside is 3nm layer, 17nm layer, and 26nm layer. The working temperature of the membrane separation is room temperature, and the operating pressure is 1.8MPa. 44% of the volume to produce a high-concentration microbial flocculant; then vacuum-dry the high-concentration microbial flocculant at a temperature of 68°C for 12 hours to obtain a refined product with a purity of 92.3%.

[0029] The test method is:

[0030] (1) Purity of microbial flocculant: take the microbial flocculant prepared in the present invention, and test the purity of microbial flocculant by ultraviolet scanning and SDS-PAGE protein electrophoresis;

[0031] (2) SS removal rate: In 100mL of kaolin suspension containing ...

Embodiment 2

[0036] Centrifuge the fermentation liquid of microbial cells at a speed of 2500r / min for 18 minutes. After removing most of the cells, take the supernatant for use; then use a tubular ceramic ultrafiltration membrane to separate, concentrate and purify the supernatant. The membrane has stepped pores, the average pore diameter is 8 nm, and the tube pore diameter distribution from the inside to the outside is 3nm layer, 17nm layer, and 22nm layer. The working temperature of the membrane separation is room temperature, the operating pressure is 1MPa, and the volume of the concentrated fermentation broth is the original volume 35% of the high-concentration microbial flocculant was obtained; then the high-concentration microbial flocculant was vacuum-dried at a temperature of 65°C for 14 hours to obtain a refined product with a purity of 91.4%.

[0037] The test method is consistent with Example 1, and the obtained data are shown in Table 1.

Embodiment 3

[0039] Centrifuge the fermentation liquid of microbial cells at a speed of 3600r / min for 13 minutes, remove most of the cells, and take the supernatant for later use; then use a tubular ceramic ultrafiltration membrane to separate, concentrate and purify the supernatant, The membrane has stepped pores, the average pore diameter is 25 nm, and the pore diameter distribution from the inside to the outside is 4nm layer, 19nm layer, and 27nm layer. The working temperature of the membrane separation is room temperature, the operating pressure is 2MPa, and the volume of the concentrated fermentation broth is the original volume 45% of the high-concentration microbial flocculant was obtained; then the high-concentration microbial flocculant was vacuum-dried at a temperature of 75°C for 10 hours to obtain a refined product with a purity of 93.5%.

[0040] The test method is consistent with Example 1, and the obtained data are shown in Table 1.

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Abstract

The invention belongs to the technical field of sewage processing, and provides a method for improving purity of a microbial flocculant for sewage treatment. The method comprises the steps of: separating a microorganism broth by centrifugation, and taking a supernatant to concentrate and purify the material by using an ultrafiltration membrane to obtain the high-concentration microbial flocculant,and then performing vacuum drying treatment to obtain a high-purity refined product. Compared with a conventional method, the method for purifying the microbial flocculant of the invention utilizes an ultrafiltration membrane concentration treatment technology to overcome the defects of complicated traditional process steps, the quality of the microbial broth after clarification and filtration can be greatly improved, the product yield can be improved, and the obtained microbial flocculant product has high purity, good stability and strong flocculation ability, and can be widely used in industrial production.

Description

technical field [0001] The invention belongs to the technical field of sewage treatment and provides a method for improving the purity of microbial flocculants for sewage treatment. Background technique [0002] At present, the flocculants widely used in industrial wastewater treatment, drinking water purification, sludge dehydration, food processing and fermentation industries mainly include the following categories: (1) Inorganic flocculants, such as iron salts, aluminum salts and their polymers; (2) Organic polymer flocculants, such as polyacrylamide (PAM); (3) Composite flocculants, such as polyaluminum ferric chloride-chitosan (PAFC-CTS). However, these kinds of chemical flocculants introduce a large amount of ions, have certain toxicity, produce secondary pollution and are not easy to remove. [0003] Microbial flocculant is a biodegradable metabolite produced by microorganisms or their secretions. It is a safe, efficient, non-toxic, and non-secondary pollution water ...

Claims

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Application Information

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IPC IPC(8): B01D61/14C02F3/34
CPCB01D61/145C02F3/34
Inventor 陈庆何方
Owner CHENDU NEW KELI CHEM SCI CO LTD
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