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1-Hexadecanoyl-sn-glycerol-phosphorylcholine and dodecanedioic acid as diagnostic markers for idiopathic male infertility and their application

A technology for dodecanedioic acid and male infertility, applied in the fields of analytical chemistry and clinical medicine, can solve the problems of limited application, increased instrument occupancy, long detection time, etc., to improve sensitivity and specificity, and prevent disease progression. , the effect of delaying disease progression

Active Publication Date: 2021-08-17
NANJING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, gas chromatography tandem mass spectrometry is limited to the detection of volatile substances, and the detection range cannot cover the requirements of metabolic profiling
In order to make up for this defect, gas chromatography tandem mass spectrometry often uses a derivatization method to detect chemicals, which makes the pretreatment method complicated and introduces more errors
At the same time, due to the limitation of separation technology, gas chromatography tandem mass spectrometry detection generally takes a long time, which prolongs the experimental period and increases the occupancy rate of the instrument, which limits its application in the detection of large sample populations.
Capillary electrophoresis tandem mass spectrometry has advantages in the separation and detection of polar compounds, but the problem of instrument stability is difficult to solve

Method used

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  • 1-Hexadecanoyl-sn-glycerol-phosphorylcholine and dodecanedioic acid as diagnostic markers for idiopathic male infertility and their application
  • 1-Hexadecanoyl-sn-glycerol-phosphorylcholine and dodecanedioic acid as diagnostic markers for idiopathic male infertility and their application
  • 1-Hexadecanoyl-sn-glycerol-phosphorylcholine and dodecanedioic acid as diagnostic markers for idiopathic male infertility and their application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0106] Embodiment 1: Research object selection and group basis

[0107]The subjects of this part of the study are from the first-diagnosed idiopathic male infertility cases and healthy childbearing controls in the Affiliated Hospital of Nanjing Medical University. The research content and the informed consent form were approved by the Ethics Committee of Nanjing Medical University and complied with the requirements of relevant laws and regulations. Cases and controls signed the informed consent after understanding the contents. All subjects underwent a complete physical examination and completed a questionnaire including basic personal information, living habits, occupational and environmental exposures, genetic risk factors, sexual and reproductive function, disease history, and physical activity. The first stage included 607 idiopathic male infertility cases and 430 healthy controls who met the requirements; the second stage included 15 idiopathic male infertility cases and...

Embodiment 2

[0138] Example 2: UPLC-MS metabolomics biomarker screening for idiopathic male infertility

[0139] 1. Sample pretreatment

[0140] 1.1. Take 300 μL of urine, add 10 μL of internal standard A, add 10 μL of internal standard B, add 10 μL of internal standard C, add 40 μL of methanol (reagent A), and vortex for 30 seconds.

[0141] 1.2. Centrifuge at 16000g at 4°C for 15min in a centrifuge, transfer the supernatant to a 1.5mL imported EP tube, and concentrate the supernatant to dryness in a centrifugal concentrator at room temperature.

[0142] 1.3. Reconstitute with 5 μL ultrapure water (reagent D) and wait for analysis.

[0143] 2. Instrument testing

[0144] 2.1. Analytical instruments: UPLC Ultimate 3000 system (Dionex) high-performance liquid chromatography; Q-Exactive high-resolution mass spectrometer.

[0145] 2.2. Liquid phase conditions:

[0146] 2.2.1 The liquid chromatographic column is a Hypersil GOLD C18 chromatographic column (100mm×2.1mm, particle size 1.9μm, ...

Embodiment 3

[0159] Example 3 Stability analysis of 1-hexadecanoyl-SN-glycerol-phosphorylcholine and dodecanedioic acid in urine

[0160] The stability of the levels of 1-hexadecanoyl-SN-glycerol-phosphorylcholine and dodecanedioic acid in urine was evaluated by the method of Example 2 (the interval was 2 weeks). The results showed that the levels of 1-hexadecanoyl-SN-glycerol-phosphocholine and dodecanedioic acid in urine were stable ( figure 2 ), with properties as diagnostic / monitoring markers.

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Abstract

The invention belongs to the fields of analytical chemistry and clinical medicine, and discloses 1-hexadecanoyl-SN-glycerol-phosphorylcholine and dodecanedioic acid as diagnostic markers for idiopathic male infertility and applications thereof. The markers are urine 1-hexadecanoyl-SN-glycerol-phosphorylcholine and dodecanedioic acid, which have high sensitivity and specificity in the diagnosis of idiopathic male infertility, and can be used to prepare idiopathic male infertility Diagnostic or monitoring reagents for male infertility.

Description

[0001] field of invention [0002] The invention belongs to the field of analytical chemistry and clinical medicine, and relates to 1-hexadecanoyl-SN-glycerol-phosphocholine and dodecanedioic acid in urine related to idiopathic male infertility as biomarkers and its Detection method and application based on UPLC-Q active MS. Background technique [0003] Currently, about 10-15% of couples of reproductive age worldwide suffer from fertility disorders. Due to the large population base in my country, the number of infertility patients among newlyweds is far more than one million, and the infertility caused by male factors is higher than 50%. There are still about 40%-75% of male infertility whose cause cannot be found, which is called idiopathic male infertility. Idiopathic male infertility poses a disproportionately high disease burden. [0004] In fact, the diagnosis of idiopathic male infertility is very difficult. The WHO diagnostic criteria for male infertility are coupl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/72
CPCG01N30/02G01N30/72
Inventor 陈敏健丁兴旺胡艳辉卢婷庄婷钰管昱盛
Owner NANJING MEDICAL UNIV
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