Lactobacillus plantarum strain BX62 with antioxidant ability and application thereof

A technology of Lactobacillus plantarum and antioxidant capacity, applied in the application of Lactobacillus plantarum strain BX62, the field of Lactobacillus plantarum strain BX62, can solve problems such as damage to biological macromolecules, achieve damage reduction, antioxidation and scavenging free radicals, improve The effect of the body's immunity

Inactive Publication Date: 2019-01-18
甘肃普诺贝康生物科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Oxidation is a necessary metabolic process for the body, but excessive oxidation can cause damage to biological macromolecules

Method used

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  • Lactobacillus plantarum strain BX62 with antioxidant ability and application thereof
  • Lactobacillus plantarum strain BX62 with antioxidant ability and application thereof
  • Lactobacillus plantarum strain BX62 with antioxidant ability and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1: Isolation and identification of lactic acid bacteria from traditional fermented yak yoghurt and silage on the Qinghai-Tibet Plateau

[0043] 1. Sample source

[0044] It comes from traditional fermented yak dairy products and silage in Tianzhu Tibetan Autonomous County, Gansu Province, my country.

[0045] 2. Isolation of lactic acid bacteria from traditional yak dairy products and silage

[0046] Take 1mL of fermented yak milk product and dilute it with sterilized saline at a ratio of 1:10, which is 10 -1 dilution. Then draw 1mL of the above diluent, and then dilute it 10 times, which is 10 -2 dilution, and so on. Pick 10 -5 、10 -6 、10 -7 For three dilutions, draw 100 μL of the dilutions and spread them evenly on the MRS, M17, KFS and SL (adjust pH 5.4) plate culture medium respectively. Cultivate for 48 hours, pick characteristic colonies, and further purify to obtain pure isolated lactic acid bacteria strains.

[0047] 3. Identification of strains...

Embodiment 2

[0054] Embodiment 2: Screening of high antioxidant capacity lactic acid bacteria

[0055] 1. Preparation of complete cell and cell-free extracts and fermentation supernatant of lactic acid bacteria

[0056] Preparation of cell bacterial liquid: the bacterial liquid stored at -80°C in the laboratory was inserted into the sterilized MRS medium and cultivated overnight at 37°C, and was continuously passaged for 3 times. The culture solution was collected by centrifugation at 12,000 g, washed with PBS three times, resuspended in PBS, and the number of bacteria was adjusted.

[0057] Preparation of cell-free extract: activate the bacterial solution twice overnight at 37°C, centrifuge at 8000g, 10min, 4°C, collect the bacterial cells, wash with PBS for 3 times, resuspend in PBS, and adjust the number of bacteria to 1×10 9 CFU / mL or 1×10 10 CFU / mL. Sonicate the cells in an ice bath, 5s, 5s, 30min, 360W. 8000g, 10min, 4°C, and the supernatant was collected as a cell-free extract (...

Embodiment 3

[0082] Example 3: In vitro studies on probiotic properties

[0083] 1. The digestive tract simulation test and hydrophobicity determination of the invented strain

[0084] (1) Hydrophobic ability

[0085] According to the method of Mansona et al. (1992), the activated lactic acid bacteria were inoculated into liquid MRS medium, cultivated at 37°C for 16-18h, centrifuged at 5000rpm / min, and washed with 0.1mol KNO 3 (pH6.2) After washing twice, use the solution to suspend the bacterial liquid, and measure its initial absorbance A at 600nm 0 . Then draw 3ml of bacterial suspension, add 1ml of xylene, pre-incubate at room temperature for 10min, vortex quickly mix for 2min, let stand at room temperature for 15min to separate layers, absorb the lower aqueous phase, use the buffer as a blank control, measure the final absorbance A at 600nm and Make a note.

[0086] Hydrophobic capacity (%) = (A 0 -A) / A 0 ×100%

[0087] In the formula: A 0 and A are the absorbance values ​​mea...

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Abstract

The invention discloses a Lactobacillus plantarum strain BX62 with antioxidant ability. A preservation number is CGMCC15779. And the application of the strain is also included in the invention. The Lactobacillus plantarum strain BX62 is isolate from that traditional fermented yak yoghurt of the Qinghai-Tibet plateau, and is prove to be capable of remarkably improving the T-AOC, GSH-Px, T-SOD activity, and decrease the content of MDA in mice serum. The strain has good antioxidant ability and can effectively promote the activity of antioxidant enzymes in vivo. The hydrophobic ability of the strain increases the chance for it to adhere to the intestinal wall and play a probiotic role. The strain has no toxic and side effects and can be used in the preparation of fermented foods and health products, cosmetics and medicines.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the field of development and application of functional lactic acid bacteria, in particular to a strain of Lactobacillus plantarum strain BX62 with high antioxidant capacity, and the invention also includes the application of the Lactobacillus plantarum strain BX62. Background technique [0002] Oxidation is a necessary metabolic process of the body, but excessive oxidation can cause damage to biological macromolecules. Oxidative stress is the root cause of body aging and aging-related diseases. [0003] Free radicals have a strong oxidative effect, which can trigger lipid peroxidation, damage the cell membrane, and cause cell death. When the human body produces excessive free radicals, the free radicals will combine with biological macromolecules such as proteins and nucleic acids in human cells, and destroy their structures and functions. The specific harms of free radicals to peo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02A23L33/135A23L29/00A23C9/123A61K35/747A61P39/06A61P37/04A61Q19/08C12R1/25
CPCA61P37/04A61P39/06A61Q19/08A23C9/1234A23L29/065A23L33/135A61K8/99A61K35/747C12N1/02C12N1/20A23V2002/00C12R2001/25C12N1/205A23V2400/169A23V2200/324A23V2200/30
Inventor 郭旭生丁武蓉王丽娜柯文灿
Owner 甘肃普诺贝康生物科技有限责任公司
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