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A method for producing high-purity crystalline maltose

A technology of maltose and maltose amylase, which is applied in the field of food and pharmaceutical starch sugar production, can solve the problems of high-purity crystalline maltose production starting late and high price, and achieve stable product quality, improve crystallization purity, and realize the effect of industrialized production

Active Publication Date: 2019-01-18
湖南金代科技发展有限公司
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The production of high-purity crystalline maltose in my country started relatively late. In China, only Henan Baofeng and Jiangsu Aogu produce a small part of crystalline maltose, with a content of only about 92%. It is only used in some oral preparations and foods, and it is used in the preparation of intravenous injections. The raw material medicine of maltose is only imported, and the price is expensive, more than 400,000 yuan per ton

Method used

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  • A method for producing high-purity crystalline maltose
  • A method for producing high-purity crystalline maltose

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Experimental program
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Effect test

Embodiment 1

[0026] Synthesize the gene sequence encoding the maltogenic amylase shown in SEQ ID NO.1, connect it to pET24a, transform the ligated product into Escherichia coli JM109 competent cells, extract the plasmid for verification, the result is correct, and transform the plasmid into Escherichia coli BL21 (DE3) competent cells For the cells, the transformants were picked and cultured overnight at 37° C. in LB liquid medium (containing 100 mg / L kanamycin). Pick positive clones, cultivate seed liquid, and transfer to TB liquid fermentation medium. After culturing at 37°C for 2 hours, add 0.01mM IPTG at a final concentration for induction, and continue to culture and ferment on a shaker at 25°C for 48 hours, then centrifuge the fermentation broth at 4°C and 8000rpm for 10 minutes to remove bacteria, and collect the fermentation supernatant.

[0027] Slowly add 50% (NH 4 ) 2 SO 4 , placed overnight at 4°C, centrifuged at 8000rpm for 20min at 4°C, and collected the precipitate. After...

Embodiment 2

[0029] (1) Prepare 20% (w / v) potato starch solution, adjust pH to 5.5, add 30U / g dry starch α-amylase, add 24U / g dry starch pullulanase and 10U after spray liquefaction treatment β-amylase / g dry starch, stirred at 60°C for 24 hours, and carried out a saccharification. Then add 20U / g maltose amylase of dry starch, stir at 60°C for 8 hours, and carry out secondary saccharification. When the saccharification is finished, the measured maltose content is 96.4%.

[0030] (2) Decolorization and filtration: heat up to kill enzymes, add activated carbon to the saccharification solution for decolorization, and filter through filter paper to make the sugar solution clear and transparent, free of foreign matter and impurities visible to the naked eye.

[0031] (3) Purification and concentration: the mass concentration of the sugar solution is concentrated to 70% by anion-cation exchange resin treatment to a conductivity of 30 μs / cm.

[0032] (4) Stirring crystallization: 1.5% by mass of ...

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Abstract

The invention discloses a production method of high-purity crystalline maltose, belonging to the field of food and pharmaceutical starch sugar production. As the novel enzyme preparation participatesin the saccharification reaction in stages, the enzyme preparation is added in different catalytic periods in the saccharification process in different times, and the effect of saccharification is optimized, and the crystallization purity is improved by adopting the combination of the traditional cooling crystallization and the ethanol precipitation crystallization. The method of the invention hasthe advantages of stable product quality, completely reaching the 2015 edition standard of the Chinese Pharmacopoeia, realizing industrial production and filling the domestic blank through 10 repetitive experiments, wherein, the product quality is stable, and the product meets the 2015 edition standard of the Chinese Pharmacopoeia.

Description

technical field [0001] The invention relates to a production method of high-purity crystalline maltose, which belongs to the field of food and pharmaceutical starch sugar production. Background technique [0002] Maltose is made from starch or starchy substance through liquefaction and saccharification. According to the product form, it is divided into liquid maltose, maltose powder and crystallized maltose. Maltose has good anti-corrosion and thermal stability, low hygroscopicity, and various physiological functions, and is widely used in the food and pharmaceutical industry. In the food industry, it is mainly used to make hard candy, beer syrup, and stuffing syrup. The main applications in the pharmaceutical industry are: (1) Oral maltose syrup to supplement the carbon source and energy needed by the body; (2) Properly proportioned with other nutrients as a nutritional supplement; (3) Used to manufacture maltitol, which is consumed by humans After adding maltitol, it wi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/22C12P19/14C12P19/12
CPCC12P19/12C12P19/14C12P19/22
Inventor 谢艳萍邓希张国军
Owner 湖南金代科技发展有限公司
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