A kind of preparation method of natural melanin nanoparticle
A technology of natural melanin and nanoparticles, which is applied in the field of nanomaterial preparation, can solve the problems of inhomogeneous supersaturation, difficulty in rapid and uniform mixing of solutions, uneven particle size distribution, etc. Effect
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Embodiment 1
[0063] The apricot core shell was washed with distilled water, and dried in a blast oven at 70°C for 2 days. Crumble the apricot core shell with a grinder, and pass through a 20-mesh sieve to obtain the apricot core shell powder, which is stored under dry conditions at room temperature.
[0064] Weigh 1 g of dried apricot core-shell powder, add it to 20 mL of 2 mol / L NaOH solution, extract with 60 Hz, 200 W ultrasonic wave for 60 min, use a circulating water vacuum pump to remove insoluble matter, and collect the filtrate to obtain a crude melanin extract.
[0065] Adjust the pH value of the crude melanin extract to 1.5-2.0 with 7mol / L hydrochloric acid solution, centrifuge at 11000r / min to remove the supernatant, and collect the precipitate.
[0066] Dilute the precipitate with distilled water, adjust the pH value to 4.5-5.0, add cellulase at a mass ratio of 0.2%, and place it in a water bath at 50-55°C for enzymolysis reaction for 60 minutes. The enzymatic hydrolysis produc...
Embodiment 2
[0077] The apricot core shell was washed with distilled water, and dried in a blast oven at 70°C for 2 days. Crumble the apricot core shell with a grinder, and pass through a 20-mesh sieve to obtain the apricot core shell powder, which is stored under dry conditions at room temperature.
[0078] Weigh 1g of dried apricot core-shell powder, add it to 15mL of 3mol / L NaOH solution, extract with 60Hz, 200W ultrasonic wave for 90min, remove insoluble matter by suction filtration with a circulating water vacuum pump, collect the filtrate to obtain crude melanin extract.
[0079] Adjust the pH value of the crude melanin extract to 1.0-1.5 with 5 mol / L hydrochloric acid solution, centrifuge at 9000 r / min, remove the supernatant and collect the precipitate.
[0080] Dilute the precipitate with distilled water, adjust the pH value to 4.5-5.0, add cellulase at a mass ratio of 0.4%, and place it in a water bath at 50-55°C for 90 minutes for enzymatic hydrolysis. The enzymatic hydrolysis ...
Embodiment 3
[0087] Take the pure melanin prepared in Example 1 or 2, dissolve it in 0.1 mol / L NaOH solution, and prepare a 25 μg / mL melanin solution.
[0088] Adjust the pH value of the solution to 7.0 with 1mol / L HCl, and ultrasonically treat with 200W, 60Hz ultrasonic wave for 30min, stirring continuously during this period; then, use an ultrasonic cell crusher with a power of 200W to crush for 10min, during the crushing process, sonicate for 1s, stop 2s.
[0089] Use 7mol / L HCl to adjust the pH value of the solution to 1.5~2.0, centrifuge at 11000r / min to remove the supernatant, collect the solid matter, and use a vacuum freeze dryer to vacuum freeze-dry under the conditions of -40~-45°C and 5~13Pa. , to obtain natural melanin nanoparticles MNPs, stored at 4°C.
[0090] Figure 5 , Image 6 The particle size distribution and TEM images of the prepared MNPs are given respectively. It can be seen that the MNPs have a particle size range of 10 ± 5 nm and have good dispersion stability...
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