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Water storage porous silicon dioxide magnetic particles as well as preparation process and application thereof

A technology of porous silica and magnetic particles, which is applied in the direction of DNA preparation, biochemical equipment and methods, and the measurement/testing of microorganisms, etc. It can solve the problems of nucleic acid concentration and purity decline, and achieve the effect of high binding capacity

Active Publication Date: 2019-02-15
SUZHOU ENRICHING BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, this method will also bring some problems. The purity and concentration of nucleic acid are not as high as those of the two-step method (cleavage, combination and separation). This is because the release of nucleic acid from cells also releases a lot of impurities. Impurities will also be adsorbed to the surface of the magnetic particles, which makes it difficult for the nucleic acid released from the cells to bind to the surface of the magnetic particles. As a result, the concentration and purity of the nucleic acid will decrease to a certain extent, which will lead to some false negatives

Method used

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  • Water storage porous silicon dioxide magnetic particles as well as preparation process and application thereof
  • Water storage porous silicon dioxide magnetic particles as well as preparation process and application thereof
  • Water storage porous silicon dioxide magnetic particles as well as preparation process and application thereof

Examples

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Embodiment 2

[0055] Another specific example of preparing superparamagnetic ferric oxide particles is provided below:

[0056] Dissolve 2.8g of ferric chloride hexahydrate into 100mL of tetraethylene glycol, add 1.6g of polyacrylic acid (mw=500) and 5.2g of sodium acetate, stir well, add to the polytetrafluoroethylene reactor, put Into the oven at 200 ° C for 10 hours. After cooling to room temperature, it was cleaned with deionization, and dried at 60°C to obtain 120nm monodisperse superparamagnetic ferric oxide particles.

Embodiment 3

[0058] Provide a kind of specific embodiment of preparing superparamagnetic ferric oxide particle below:

[0059] Add 10 g of ferric chloride hexahydrate and 4.5 g of ferrous chloride tetrahydrate into 1 L of deionized water, stir mechanically at 500 rpm for 30 min, add 50 mL of aqueous ammonia solution, continue mechanical stirring for 1 hour, and clean the magnetic separation with deionized properties, to obtain 15nm magnetic ferric oxide particles.

Embodiment 4

[0061] Provide a kind of specific embodiment of preparing superparamagnetic ferric oxide particle below:

[0062] Add 50g of ferric chloride hexahydrate and 24g of ferrous chloride tetrahydrate into 2L of deionized water, stir mechanically at 500rpm for 30min, add 100mL of 1M sodium hydroxide solution, raise the temperature to 80°C, continue mechanically stirring for 2 hours, cool down After reaching room temperature, the magnetic separation was washed to neutrality with deionization to obtain 30nm magnetic ferric oxide particles.

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Abstract

The invention discloses water storage porous silicon dioxide magnetic particles as well as a preparation process and application thereof. The water storage porous silicon dioxide magnetic particles comprise the following raw materials: a magnetic core, silicone, silica sol, a pH adjusting substance and a polylactic acid-glycolic acid copolymer. In the reaction composition, the pH regulator can continuously deposit silicone and silica sol on the surface of the magnetic core, and the polylactic acid-glycolic acid copolymer adsorbs the silica sol and causes a silicon dioxide coating to form a reticular porous structure, thereby forming magnetic particles with a diameter of 10[mu]m or less. The silicon magnetic particles provided by the invention have a higher binding capacity to nucleic acidsand less adsorption to salt ions, proteins or other impurities, have specific porosity, and the binding capacity and binding specificity can be obtained by selectively changing parameters of a synthesis reaction.

Description

technical field [0001] The invention relates to the field of biological nanomaterials, in particular to a water-storage porous silicon dioxide magnetic particle and its preparation process and application. Background technique [0002] The isolation of nucleic acids is one of the important steps in molecular diagnostics. The quality and quantity of nucleic acids isolated from samples greatly affect the success of downstream diagnostic applications, and clinical applications also require rapid and automated isolation procedures. Some types of clinical and environmental samples present particular challenges to the successful isolation of nucleic acids, such as degradation of target nucleic acids during vigorous sample handling that can lead to false negative results in downstream detection assays, jeopardizing the purity and yield of extracted nucleic acids. Higher requirements on the rate can make the downstream detection have better sensitivity and reliability. [0003] Th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L67/04C08K13/06C08K7/26C08K3/22C12N15/10C12Q1/6806
CPCC08K3/22C08K7/26C08K13/06C08K2003/2275C08K2201/003C08K2201/006C08K2201/01C08K2201/011C12N15/1013C12Q1/6806C08L67/04
Inventor 张佳斌刘栋然曲峰尚春庆
Owner SUZHOU ENRICHING BIOTECH CO LTD
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