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Composition for remediating arsenic-phenanthrene complex contaminated soil and application thereof

A compound pollution and composition technology, applied in the restoration, application, and fertilizer mixture of polluted soil, can solve the problems of the limitation of pollutant treatment capacity and the influence of centipede grass on the absorption of arsenic, so as to achieve rapid and efficient treatment and promote the degradation of phenanthrene The ability to promote the effect of soil remediation

Pending Publication Date: 2019-02-26
中国地质环境监测院 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, arsenic inhibits the growth of Agrobacterium in the later stage, which in turn affects the absorption of arsenic by centipede grass. Contaminated soil is often accompanied by a variety of pollutants, and the ability of centipede grass to treat pollutants is limited.

Method used

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  • Composition for remediating arsenic-phenanthrene complex contaminated soil and application thereof
  • Composition for remediating arsenic-phenanthrene complex contaminated soil and application thereof
  • Composition for remediating arsenic-phenanthrene complex contaminated soil and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] A composition for remediating arsenic-phenanthrene complex polluted soil, comprising centipede grass, microbial agent and glutathione, the method for using said composition to remediate polluted soil comprises the following steps:

[0049] (1) Microbial agent preparation: Activate Alcaligenes faecalis for 2 generations, then inoculate it in a fermenter for fermentation, the temperature of the fermentation is 32°C, the tank pressure of the fermenter is 0.03MPa, and the stirring speed is 330rpm. The time is 15 hours, and finally the fermentation broth is centrifuged at 4°C and 6500rpm for 10 minutes, the culture medium is removed, and sterile water is added to the cell sediment and mixed to obtain the microbial agent. The concentration of the microbial agent is 1×10 8 CCU / mL;

[0050] (2) Centipede Grass cultivation: when the Centipede Grass spores were cultivated to grow 6 pinnate leaves, they were transplanted into the soil of arsenic-phenanthrene compound pollution;

...

Embodiment 2

[0056] A composition for remediating arsenic-phenanthrene complex polluted soil, comprising centipede grass, microbial agent and glutathione, the method for using said composition to remediate polluted soil comprises the following steps:

[0057] (1) Preparation of microbial bacterial agent: Activate Alcaligenes faecalis for 1 generation, then inoculate it in a fermenter for fermentation, the temperature of the fermentation is 30°C, the pressure of the fermenter is 0.02MPa, the stirring speed is 350rpm, and the fermented The time is 18 hours, and finally the fermentation broth is centrifuged at 3°C ​​and 7000rpm for 8 minutes, the culture medium is removed, and sterile water is added to the cell sediment and mixed evenly to obtain the microbial inoculum. The concentration of the microbial inoculum is 2.5×10 8 CCU / mL;

[0058] (2) Centipede Grass cultivation: when the Centipede Grass spores were cultivated to grow 5 pinnate leaves, they were transplanted into the soil of arseni...

Embodiment 3

[0063] A composition for remediating arsenic-phenanthrene complex polluted soil, comprising centipede grass, microbial agent and glutathione, the method for using said composition to remediate polluted soil comprises the following steps:

[0064] (1) Preparation of microbial bacterial agent: activate Alcaligenes faecalis for 3 generations, then inoculate it in a fermenter for fermentation, the temperature of the fermentation is 35°C, the tank pressure of the fermenter is 0.05MPa, and the stirring speed is 300rpm. The time is 12 hours, and finally the fermentation broth is centrifuged at 8°C and 6000rpm for 15 minutes, the culture medium is removed, sterile water is added to the cell sediment and mixed evenly to obtain the microbial agent, the concentration of the microbial agent is 0.5×10 8 CCU / mL;

[0065] (2) Centipede Grass cultivation: when the Centipede Grass spores were cultivated to grow 7 pinnate leaves, they were transplanted into the soil of arsenic-phenanthrene comp...

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Abstract

The invention belongs to the field of environmental remediation engineering, and relates to a composition for soil remediation and application thereof. The composition comprises pteris vittata, a microbial agent and glutathione. According to the invention, remediation treatment is performed on organic-inorganic substances contaminated soil through a plant-microorganism system, and pteris vittata,the microbial agent and glutathione are used in combination for achieving a synergistic effect, wherein the microbial agent can effectively promote the increment of pteris vittata biomass, thereby improving the accumulation of arsenic; pteris vittata provides attachment carriers for microorganisms, thereby promoting the degradation of phenanthrene by microorganisms; and glutathione can effectivelyeliminate the inhibition action of arsenic-phenanthrene on pteris vittata and the microorganisms, thereby obviously improving the handling capacity of pteris vittata and the microorganisms on pollutants and promoting the soil remediation.

Description

technical field [0001] The invention belongs to the field of environmental remediation engineering, and relates to a composition for remediating soil and its application, mainly to a composition for remediating arsenic-phenanthrene complex polluted soil and its application, and in particular to a plant-microorganism joint remediation of arsenic-phenanthrene Compositions of complex polluted soils and their applications. Background technique [0002] Polycyclic aromatic hydrocarbons (PAHs) and arsenic (As) are two types of pollutants that are generally concerned in the field of soil environmental protection at home and abroad, among which 7 PAHs and As, including naphthalene, fluoranthene and benzo[a]pyrene, are listed as the No. A batch of 68 priority control pollutants have been announced, all of which have strong carcinogenic, teratogenic and mutagenic properties. The polyphenylene ring conjugated system makes it highly stable, can stay in the environment for a long time a...

Claims

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Application Information

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IPC IPC(8): C09K17/14B09C1/10B09C1/00
CPCC09K17/14B09C1/10B09C1/105Y02P10/20
Inventor 孙璐朱岗辉孟晖郭聪刘可袁富强李春燕
Owner 中国地质环境监测院