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Process for treating fermented wastewater with high concentration of threonine

A technology for threonine fermentation liquid and fermentation wastewater, which is applied in the field of treating high-concentration threonine fermentation wastewater, can solve the problems of difficulty in optimizing the composition of amino acid waste liquid, restriction on utilization of amino acid waste liquid, poor field effect, etc., and achieves the realization of direct biological Utilize and improve the effect of industrial added value, strong ion exchange capacity and adsorption capacity

Pending Publication Date: 2019-03-08
许传高
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, due to the basic characteristics of the liquid, there are negative factors such as strong acidity, strong corrosiveness, heavy odor, difficulty in optimizing the composition of amino acid waste liquid, poor field effect, and even harmful to some crops, making amino acid waste liquid The use of organic fertilizers has been limited, and the fertilizers produced, whether solid waste or liquid fertilizers, are difficult to promote

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A process for treating high-concentration threonine fermentation wastewater, comprising the steps of:

[0024] Step 1) Escherichia coli engineering bacteria K12△dapA seed solution (the concentration of the seed solution is 1×10 8 cfu / mL) according to the inoculum amount of 8%, was inserted into the fermenter containing the fermentation medium for fermentation, the temperature was 30°C, the tank pressure was 0.04MPa, the ventilation rate was 0.5vvm, the rotation speed was 100rpm, and the fermentation time was 36h, and then Inject Chlamydomonas reinhardtii according to 10% inoculum (the concentration of Chlamydomonas reinhardtii is 1×10 5 cfu / mL), continue to ferment for 36 hours, stop the fermentation, and collect the fermented liquid;

[0025] The components of the fermentation medium are: glucose 20g / L, glycerol 15g / L, corn steep liquor 15g / L, ammonium sulfate 2g / L, potassium dihydrogen phosphate 0.2g / L, dipotassium hydrogen phosphate 0.2g / L, Magnesium sulfate heptah...

Embodiment 2

[0031] The impact of different factors on threonine output and acetic acid output in the fermentation method of the present invention:

[0032] Set group:

[0033] Experimental group: Example 1;

[0034] Control group 1: without adding Chlamydomonas reinhardtii, the rest are the same as in Example 1;

[0035] Control group 2: Glycerin was replaced by glucose of the same quality, and the rest were the same as in Example 1;

[0036] Control group 3: No Chlamydomonas reinhardtii was added, and glycerol was replaced by glucose of the same quality, and the rest were the same as in Example 1.

[0037] The contents of threonine and acetic acid in the final fermentation broth of each group are shown in Table 1:

[0038] Table 1

[0039] [0001] Group

[0040] Conclusion: The experimental group can use the acetic acid in the threonine fermentation broth as a carbon source to carry out non-light summing through the auxiliary fermentation treatment of Chlamydomonas reinhardt...

Embodiment 3

[0042] The fertilizer effect test of the biological organic matter prepared by the present invention:

[0043] Take tomato as an example to detect tomato yield and quality.

[0044] Experimental group: spraying biological organic matter of the present invention;

[0045] Control group: Spray the same amount of water.

[0046] The location is a greenhouse in Inner Mongolia; the planting area of ​​each plot is 10m×10m, and 3 repetitions are set up. A total of 6 experimental plots are set up and arranged in random blocks. The tomato variety is Fenli No. 1, and 100kg of liquid fertilizer is sprayed per mu, and other planting conditions are the same. The results are shown in Table 2:

[0047] Table 2

[0048] Group

[0049] Conclusion: As shown in Table 2, compared with the control group without bio-organic matter, the organic matter in the experimental group can increase the weight of single fruit, increase the proportion of soluble solids and soluble sugar, reduce t...

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PUM

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Abstract

The invention belongs to the technical field of bioenvironmental protection, and discloses a process for treating fermented wastewater with high concentration of threonine. The process includes the following steps: step 1), preparing threonine fermentation liquid, step 2), preparing protein paste; step 3), preparing the threonine; step 4), collecting threonine fermentation wastewater; step 5), preparing bioorganic matter. The threonine, the protein paste and the biological organic matter are simultaneously prepared in the fermentation process, an intermediate pretreatment step is omitted, anddirect biological utilization of sewage is realized, so that waste is turned into treasures, discharge is reduced, and economic benefits are remarkable.

Description

technical field [0001] The invention belongs to the technical field of biological environmental protection, and in particular relates to a process for treating high-concentration threonine fermentation wastewater. Background technique [0002] Threonine is an essential amino acid, which is the second limiting amino acid in pig feed and the third limiting amino acid in poultry feed. With the wide application of lysine and methionine synthetic products in compound feed, threonine has gradually become an The main limiting factor for the growth of livestock and poultry. Threonine is mainly used as a feed additive. Its production steps are concentration, crystallization, and extraction of bacterial protein. However, the mother liquor produced in the process is difficult to deal with and the cost is high. It is difficult to obtain cost-effective by-products. [0003] At present, a small part of the wastewater from the amino acid fermentation industry is used for the development o...

Claims

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Application Information

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IPC IPC(8): C12P13/08C05G3/00C05F17/00C12R1/19
CPCC12P13/08C05B7/00C05F17/00C05G5/20C05C9/00C05C11/00C05F11/02C05F11/08Y02W30/40
Inventor 赵春晓徐太海杨鑫哲曹博超
Owner 许传高
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