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Method for preparing nibea japonica scale immunomodulating peptide

A technology of immune regulation and croaker, which is applied to the preparation method of peptides, chemical instruments and methods, peptides, etc., can solve problems such as insufficient proteolysis, and achieve the effect of improving complexation, increasing production, and increasing removal effect

Active Publication Date: 2019-03-08
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention aims to overcome the problem of insufficient enzymatic hydrolysis of fish scales by proteases in the prior art, and provides a preparation method for the scale immunomodulatory peptide of Japanese yellow croaker. In the preparation method, proteases can fully enzymatically hydrolyze fish scales, thereby greatly improving immune regulation. Peptide yield

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The preparation method of the adsorbent includes the following steps:

[0026] Crab shell powder is obtained by crushing crab shell. Add crab shell powder and dopamine into water at a mass ratio of 1:0.3. The mass volume ratio of crab shell powder to water is 1:35g / mL. The temperature is raised to 50°C, and then glutaraldehyde is added. The molar ratio of aldehyde, glutaraldehyde and dopamine is 1:1.5, the reaction is stirred for 1.5h, and the stirring rate is controlled at 100r / min. After filtering and washing, the crab shell powder is dried at 60℃ for 2.5 hours to obtain pretreated crab shell Powder; the pretreated crab shell powder is soaked in 8% acetic acid aqueous solution with a mass concentration of 8% for 3 hours, washed with water to neutrality, and dried. The dried crab shells are purged under inert gas at 3.5°C / min The heating rate was raised to 330℃, roasted for 40min, and then heated to 620℃ at a heating rate of 7℃ / min, and roasted for 2h to obtain porous cra...

Embodiment 2

[0033] The preparation method of the adsorbent includes the following steps:

[0034] Crab shell powder is obtained by crushing crab shell. Add crab shell powder and dopamine into water at a mass ratio of 1:0.3. The mass volume ratio of crab shell powder to water is 1:32g / mL. The temperature is increased to 46°C, and then glutaraldehyde is added. The molar ratio of aldehyde, glutaraldehyde and dopamine is 1:1, the reaction is stirred for 1.5h, and the stirring rate is controlled at 85r / min. After filtering and washing, the crab shell powder is dried at 55°C for 2 hours to obtain pretreated crab shell powder ; The pretreated crab shell powder is soaked in a 6% acetic acid aqueous solution by mass concentration for 2.5 hours, washed with water to neutrality, and dried. The dried crab shells are purged under inert gas at 3℃ / min. The heating rate was increased to 310℃, roasted for 35min, and then heated to 620℃ at a heating rate of 6℃ / min, and roasted for 1.5h to obtain porous crab s...

Embodiment 3

[0041] The preparation method of the adsorbent includes the following steps:

[0042] Crab shell powder is obtained by crushing crab shell. Add crab shell powder and dopamine into water at a mass ratio of 1:0.4. The mass volume ratio of crab shell powder to water is 1:38 g / mL. The temperature is raised to 55°C, and then glutaric dioxide is added. The molar ratio of aldehyde, glutaraldehyde and dopamine is 1:1.5, the reaction is stirred for 2h, and the stirring rate is controlled at 100r / min. After filtering and washing, the crab shell powder is dried at 65°C for 3 hours to obtain the pretreated crab shell powder; The pretreated crab shell powder is soaked in 8% acetic acid aqueous solution with a mass concentration of 8% for 4 hours, washed with water to neutrality, and dried. The dried crab shells are purged with inert gas at a heating rate of 4℃ / min. Raise the temperature to 340°C, roast for 45min, then heat it to 640°C at a heating rate of 7°C / min, roast for 2.5h to obtain por...

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Abstract

The invention relates to the technical field of immunomodulating peptide preparation, aims to solve the problem of insufficient proteolysis in fish scales, and discloses a method for preparing nibea japonica scale immunomodulating peptides. The method for preparing the immunomodulating peptides comprises the following steps: firstly, putting fish scales into a sodium hydroxide alkali solution to remove viscous substances on surfaces of the fish scales, fat of the fish scales and other impurity substances, soaking into hydrochloric acid to remove calcium, further carrying out enzymolysis on proteins in the fish scales, and finally carrying out ultrafiltration, nanofiltration and drying in sequence, thereby obtaining the immunomodulating peptides. By adopting the method, bone bed ash contents are decomposed and damaged by using hydrochloric acid, and the proteins in the fish scales are out of protection of the bone bed ash contents, so that the enzymolysis efficiency of papain upon the proteins in the fish scales is greatly improved, the proteins in the fish scales can be subjected to sufficient enzymolysis, and the yield of the immunomodulating peptides can be increased.

Description

Technical field [0001] The invention relates to the technical field of preparation of immunomodulatory peptides, in particular to a method for preparing Japanese yellow croaker fish scale immunomodulatory peptides. Background technique [0002] The immune system is vital to our survival and has a role in defending against pathogens, but the immune system is vulnerable to many factors, including stress, unhealthy living habits, pathogens and antigens. Several drugs that have been found to regulate the human immune response include cyclosporine, tacrolimus, glucocorticoids, phytol, aristolochic acid, plumbagin and levamisole. However, due to the side effects and high cost of these drugs, their use in patients is limited, and most immunomodulatory drugs are not suitable for chronic or preventive use. Studies have shown that the oligopeptides, polypeptides, and some mixed peptides of protease hydrolysate obtained by using food-derived proteins as raw materials after commercial prote...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34C07K1/14
CPCC07K1/14C07K1/34C12P21/06
Inventor 余方苗张壮伟胡旭阳唐云平杨最素黄芳芳丁国芳
Owner ZHEJIANG OCEAN UNIV
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