A method for preparing pei functionalized green fluorescent carbon dots and a thrombin detection method based on the carbon dots

A technology of green fluorescence and detection methods, applied in chemical instruments and methods, fluorescence/phosphorescence, nano-carbon, etc., can solve the problems of reducing nucleic acid aptamers, increasing costs, complexity, etc., and the method is simple, low-cost, The effect of high quantum yield

Active Publication Date: 2021-09-28
XIANYANG NORMAL UNIV
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Problems solved by technology

However, these methods often require chemical modification of aptamers or the use of some advanced nanomaterials, fluorescein, etc. as fluorescent groups to label nucleic acid aptamers. Reduced aptamer affinity for target

Method used

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  • A method for preparing pei functionalized green fluorescent carbon dots and a thrombin detection method based on the carbon dots
  • A method for preparing pei functionalized green fluorescent carbon dots and a thrombin detection method based on the carbon dots
  • A method for preparing pei functionalized green fluorescent carbon dots and a thrombin detection method based on the carbon dots

Examples

Experimental program
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Effect test

Embodiment 1

[0039] Example 1 Preparation method of PEI functionalized green fluorescent carbon dots:

[0040] (1) Dissolve 0.5g of PEI and 0.5g of malic acid in 13mL of ultrapure water, stir evenly, place in a reaction kettle, and conduct a hydrothermal reaction at 180°C for 10 hours to obtain a brown-yellow solution;

[0041] (2) The brownish-yellow solution obtained in step (1) is suction-filtered through a filter membrane with a pore size of 0.22 μm to remove large particles of impurities, and the filtrate is vacuum-dried at 50° C. to obtain a solid powder;

[0042] (3) Add absolute ethanol to the solid powder obtained in step (2), centrifuge with a centrifuge at 16000r / min for 15min after ultrasonication, remove the supernatant, and redisperse the precipitate in ultrapure water after freeze-drying, the resulting That is the P-CDs dispersion.

[0043] figure 1 It is a transmission electron microscope picture of the P-CDs obtained in Example 1. It can be seen from the picture that th...

Embodiment 2

[0048] The preparation of embodiment 2P-CDs:

[0049] The preparation steps are the same as in Example 1, only changing the ratio of malic acid to PEI to prepare 6 kinds of P-CDs with different fluorescence quantum yields, as shown in Table 1:

[0050]

Embodiment 3

[0051] The preparation of embodiment 3P-CDs:

[0052] The preparation steps were the same as in Example 1, except that the hydrothermal reaction time and temperature were changed and the reaction was carried out at 120° C. for 12 hours to prepare P-CDs with different fluorescence intensities.

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Abstract

The invention discloses a preparation method of PEI functionalized green fluorescent carbon dots and a thrombin detection method based on the carbon dots, using malic acid and polyethyleneimine (PEI) as precursors to prepare PEI functionalized carbon dots in one step by hydrothermal method Green fluorescent carbon dots, the method is simple and easy, the raw materials are cheap and easy to obtain, the cost is low, and the output is high, which is suitable for mass production, and the prepared carbon dots have a long emission wavelength; The luminous efficiency and dispersion of carbon dots are improved, and at the same time, the surface of carbon dots can be rich in positive charges and the quantum yield is high; the detection method of thrombin based on green fluorescent carbon dots, carbon dots and aptamers are not modified, The label-free method is adopted, which greatly simplifies the synthesis steps and reduces the cost; it has the characteristics of low operating cost, simple preparation process, mild reaction conditions and easy promotion.

Description

technical field [0001] The invention belongs to the technical fields of fluorescent carbon nanomaterials and biosensing, and relates to a preparation method of PEI functionalized green fluorescent carbon dots and a thrombin detection method based on the carbon dots. Background technique [0002] Carbon dots are a new type of zero-dimensional carbon nanomaterials. In addition to excellent fluorescence properties, carbon dots also have the advantages of low toxicity, good biocompatibility, simple and mild preparation steps, easy surface modification, and abundant and cheap raw materials. Therefore, carbon dots can be used in the biomedical field as a substitute for semiconductor quantum dots and organic dyes. [0003] At present, the synthesis methods of carbon dots mainly include laser ablation, electrochemical method, pyrolysis method, acid oxidation method, microwave method, ultrasonic method and hydrothermal method. The properties of carbon dots obtained by different pre...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64C09K11/65C01B32/15
CPCC09K11/65C01B32/15G01N21/6486G01N2021/6432
Inventor 郭颖张君才张卫红
Owner XIANYANG NORMAL UNIV
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