Preparation method of ginsenoside Rk6
A technology of ginsenosides and seeds, which is applied in the field of preparation of ginsenoside Rk6, can solve problems such as inability to produce large quantities, and achieve the effects of fast response, strong specificity, and mild and controllable reaction conditions
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Embodiment 1
[0039] Embodiment 1 preparation method of the present invention
[0040] (1) Preparation of Ganoderma lucidum seed solution: the medium formula is glucose 2%, KH 2 PO 4 0.1%, MgSO 4 0.1%, peptone 0.5%, yeast powder 0.2%, vitamin B 1 0.1%, balance water, pH natural. Put the above substances in a large beaker, add an appropriate amount of water to make a seed liquid, and after the seed liquid is cooled, divide it into 250ml wide-mouth bottles, wrap it up, put it in a pressure steam sterilizer, and sterilize it at 121°C for 30 minutes . After cooling down, insert 3-4 pieces of strains on the preserved Ganoderma lucidum slant with an inoculation loop with a diameter of 1mm in the aseptic operating table. Put the liquid culture medium connected with Ganoderma lucidum into a constant temperature shaking incubator, and cultivate it in the dark for 5-7 days at 150r / min, 28°C, and 40% humidity, until the seed liquid is clarified and there are a large number of uniformly sized star...
Embodiment 2
[0055] Example 2 Different bacterial species and RK in the fermentation product of Panax notoginseng 6 content comparison
[0056] Using thirteen kinds of medicinal fungi (boletus, fungus, shiitake 1500, oyster mushroom, pleurotus eryngii, large green mushroom, white spirit mushroom, tree chicken, tree tongue, Yunzhi, Ganoderma lucidum, Grifola frondosa, white tree flower) respectively Inoculated into the Panax notoginseng solid medium for fermentation, other conditions are the same, with reference to the steps (1) to (3) of Example 1, carry out, wherein, the water content of the Panax notoginseng solid medium is 50%, and the ganoderma seed liquid V (ml ): Panax notoginseng solid medium m (g) = 1:1, the temperature of fermentation culture is 24°C. The solid medium was observed, the results are shown in Table 3, and the RK in the fermentation product was detected 6 The results are shown in Table 4.
[0057] Table 3 Fermentation conditions of different strains and Panax notog...
Embodiment 3
[0061] Embodiment 3 best process optimization test
[0062] In this embodiment, the process conditions of steps (2) to (3) are optimized by using an orthogonal test, and the water content (%) of the solid culture medium of A. (g), C fermentation culture temperature (°C) is an influencing factor, the factor level table is shown in Table 5, the results of the orthogonal test are shown in Table 6, and the comprehensive score analysis of variance is shown in Table 7.
[0063] Table 5 Factor level table
[0064]
[0065] Table 6 Orthogonal experiments and results
[0066]
[0067] Table 7 Comprehensive score analysis of variance table
[0068] source of variance
sum of squared deviations
F value
significant
A
0.012
2
7.416
B
0.075
2
47.219
*
C
0.011
2
6.653
D
0.015
2
9.708
[0069] f 1-0.10 (2,2)=9.00F 1-0.05 (2,2) = 19.00
[0070] As can be seen fro...
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