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Water solution for efficiently detecting Bacillus anthraci marker pyridine-2,6-dicarboxylic acid as well as preparation method and application of water solution

A technology of bacillus anthracis and dicarboxylic acid, which is applied in the direction of measuring devices, instruments, fluorescence/phosphorescence, etc., can solve the problems of long reaction cycle, high synthesis cost, cumbersome synthesis and purification steps, etc., and achieve long reaction cycle and high synthesis cost , the effect of simple method

Active Publication Date: 2019-03-15
HEBEI UNIV OF TECH
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, the research on the detection of pyridine-2,6-dicarboxylic acid by fluorescence method has gradually increased. However, these often require the preparation of fine nanoparticles or other nanometer means, which often involve tedious synthesis and purification steps, resulting in long reaction cycles. high synthesis cost

Method used

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  • Water solution for efficiently detecting Bacillus anthraci marker pyridine-2,6-dicarboxylic acid as well as preparation method and application of water solution
  • Water solution for efficiently detecting Bacillus anthraci marker pyridine-2,6-dicarboxylic acid as well as preparation method and application of water solution
  • Water solution for efficiently detecting Bacillus anthraci marker pyridine-2,6-dicarboxylic acid as well as preparation method and application of water solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Accurately weigh 15.1 mg of sodium polyacrylate (average relative molecular mass: 5,000,000 to 7,000,000) into a 100 mL beaker, add 19,600 μL of water, stir at room temperature for 50 minutes until completely dissolved without bubbles, and then add 400 μL of 0.1M EuCl 3 ·6H 2 O aqueous solution, continue stirring for 30 minutes, and record it as the main solution.

[0034] (2) Take 3mL of the main body solution in the quartz cell, add 1 μL of 1.5mM 8-hydroxypyrene-1,3,6-trisodium trisulfonate solution (at this time, the 8-hydroxypyrene-1,3,6-trisodium in the quartz cell - The concentration of trisodium trisulfonate solution is 0.5 μM) (calculated as: the mass ratio is fluorescent molecular group: rare earth ion: acrylate = 1:1160:2170, and the mass fraction of water in the solution is 99.90%), stirring Evenly, the fluorescence emission spectrum of the blank was measured 11 times at an excitation wavelength of 280nm. Add different concentrations of pyridine-2,6-dic...

Embodiment 2

[0049] (1) Accurately weigh 19.0mg of sodium polyacrylate into a 100mL beaker, add 19600μL of water, stir at room temperature for 50 minutes until completely dissolved without bubbles, and then add 400μL of 0.1M TbCl 3 Aqueous solution, continue to stir for 30 minutes, recorded as the main solution.

[0050] (2) Take 3mL of the main body solution in a quartz cell, (calculated as: the mass ratio is rare earth ion: acrylate = 100:220, the mass fraction of water in the solution is 99.90%) and stir evenly, and the excitation wavelength is 280nm Measure the fluorescence emission spectrum of the blank 11 times. Add different concentrations of pyridine-2,6-dicarboxylic acid (DPA) solutions with a 1 μL injector (fluorescence titration). The fluorescence emission spectrum is measured with 280nm as the maximum excitation wavelength and 544nm as the maximum emission wavelength.

[0051] Chart 2: As the concentration of pyridine-2,6-dicarboxylic acid aqueous solution increases, the corr...

Embodiment 3

[0062] Step (1) (2) (3) (4) is the same as embodiment 2, the TbCl in the step (1) 3 Aqueous solution changed to NdCl3 Aqueous solution, with other conditions unchanged, the identification and detection of pyridine-2,6-dicarboxylic acid were also carried out with the main solution. Under the ultraviolet light, the fluorescence brightness of the host solution has been significantly changed. The ratio of fluorescence intensity has also been greatly improved.

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Abstract

The invention discloses a water solution for efficiently detecting a Bacillus anthraci marker pyridine-2,6-dicarboxylic acid as well as a preparation method and application of the water solution. Thesolution comprises the following compounds: sodium polyacrylate, rare earth ions, fluorescent molecular groups and water; the sodium polyacrylate, rare earth ions and fluorescent molecular groups aretaken as solutes, and water is taken as a solvent, wherein the mass ratio of the fluorescent molecular groups to rare earth ions to acrylic acid radicals is 1:(100 to 10000):(200 to 20000), and the mass fraction of water in the solution is 98.50% to 99.98%. The existence of pyridine-2,6-dicarboxylic acid can be quickly and accurately judged through visual inspection and simple fluorescence intensity contrast.

Description

technical field [0001] The invention belongs to the field of rare earth luminescence, and specifically relates to an aqueous solution for efficiently detecting pyridine-2,6-dicarboxylic acid, a marker of Bacillus anthracis, and a preparation method and application thereof. Through the significant enhancement of fluorescence under ultraviolet light, trace amounts of pyridine-2,6-dicarboxylic acid can be quickly detected with a detection limit of 10.3nM; and in phthalic acid, isophthalic acid, terephthalic acid, nicotinic acid, Under the interference of isonicotinic acid, 2-pyridinecarboxylic acid, benzoic acid, L-glutamic acid, L-aspartic acid and nicotinamide adenine dinucleotide, only pyridine-2,6-dicarboxylic acid was recognized, while at 10 times It also has good anti-interference ability under the coexistence of interfering substances, so as to achieve the purpose of distinction. technical background [0002] Anthrax is the first pathogen discovered in human history, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432G01N2021/6443
Inventor 李志强李焕荣刘潇杨大清
Owner HEBEI UNIV OF TECH
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