Protein-photosensitizer co-transmission pH responsive polyamino acid nanogel and preparation method thereof

A polyamino acid and nanogel technology, applied in the field of biomedicine, can solve the problems of low protein loading efficiency, low photosensitizer transfer efficiency, poor protein escape ability, etc., achieve good biocompatibility and biodegradability, improve Efficient and reproducible results in cancer treatment

Active Publication Date: 2019-03-22
JILIN INST OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a kind of protein and photosensitizer co-delivery pH-responsive polyamino acid nanogel and its preparation method in order to overcome the defects existing in the prior art. , poor protein escape ability, low photosensitizer transfer efficiency, poor diagnostic sensitivity, etc., and realize the function of simultaneous treatment of proteins and photosensitizers

Method used

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  • Protein-photosensitizer co-transmission pH responsive polyamino acid nanogel and preparation method thereof
  • Protein-photosensitizer co-transmission pH responsive polyamino acid nanogel and preparation method thereof
  • Protein-photosensitizer co-transmission pH responsive polyamino acid nanogel and preparation method thereof

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Effect test

Embodiment 1

[0039] (1) During the preparation process, 10g of L-glutamic acid γ-benzyl ester and 6.3g of triphosgene were placed in a 250mL three-necked flask with a magnetic rotor, and were poured into 100mL of anhydrous tetrahydrofuran to dissolve it completely, and the reaction The mixture was reacted in a 60°C oil bath for 1.5 hours under nitrogen protection. Subsequently, the reaction solution was dropped into 1L of frozen n-hexane, and then put into a -20 degree refrigerator for recrystallization overnight. The next day, the mixed solution was filtered and recrystallized twice with a mixed solution of ethyl acetate and n-hexane (1:2 by volume) at -20°C. Finally, the solution was filtered and the remaining solid was vacuum dried to obtain pure BLG-NCA monomer.

[0040] (2) 1g single-ended polyethylene glycol monomethyl ether (mPEG-NH 2 , the average molecular weight is 5000) and 2.7g BLG-NCA were dissolved in 10mL of anhydrous chloroform, and the reaction solution was reacted at ro...

Embodiment 2

[0046] (1) During the preparation process, 10g of L-aspartate α-benzyl ester and 6.5g of triphosgene were placed in a 250mL three-necked flask with a magnetic rotor, and were poured into 100mL of anhydrous tetrahydrofuran to dissolve it completely, and the reaction The material was reacted for 1.5 hours in a 55 degree oil bath under the condition of nitrogen protection. Subsequently, the reaction solution was dropped into 1L of frozen n-hexane, and then put into a -20 degree refrigerator for recrystallization overnight. The next day, the mixed solution was filtered and recrystallized twice with a mixed solution of ethyl acetate and n-hexane (1:3 by volume) at -20°C. Finally, the solution was filtered and the remaining solid was vacuum dried to obtain pure BLA-NCA monomer.

[0047] (2) 1g single-ended polyethylene glycol monomethyl ether (mPEG-NH 2 , the average molecular weight is 5000) and 2.7g BLA-NCA were dissolved in 10mL of anhydrous chloroform, and the reaction solutio...

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Abstract

The invention discloses a protein-photosensitizer co-transmission pH responsive polyamino acid nanogel and a preparation method thereof and belongs to the technical field of biological medicines. Forthe prepared pH responsive polyamino acid nanogel, by taking methoxy polyethylene glycol with an amido group on a single end as a macroinitiator to initiate ring opening polymerization of gamma-benzyl-L-glutamate-N-carboxanhydride or gamma-benzyl-L-aspartate-N-carboxanhydride with one step to prepare a polyamino acid main skeleton, then the tail end of the main skeleton is used for grafting a photosensitizer, and a side chain is modified with cationic molecules to add the protein supporting ability and the lysosome escape ability; and finally, a pH responsive polyamino acid nanogel system is constructed with a core crosslinking reaction, so that the purposes of in-vivo safety transfer, targeted delivery, rapid cellular uptake, in-vivo protein release and photosensitizer activation are realized, and promotion of development of combined therapy of the proteins and photodynamic therapy in the medical field can be facilitated.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a pH-responsive polyamino acid nanogel co-delivered by a protein and a photosensitizer and a preparation method thereof. Background technique [0002] Cancer has become one of the serious diseases that threaten human health and has the highest mortality rate, and shows an increasing trend year by year. According to the latest data released by the National Cancer Center in 2017, about 10,000 people are diagnosed with cancer every day in the country, and the mortality rate is as high as 60%, and cancer patients are gradually becoming younger. Therefore, it is particularly important for human development, family happiness and even social harmony to develop a cancer treatment system that is non-toxic, efficient, safe and coexistent with multiple treatments. [0003] At present, the commonly used methods for the treatment of malignant tumors are chemotherapy and radio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/06A61K47/34A61K41/00A61P35/00
CPCA61K9/06A61K41/0057A61K47/34A61P35/00
Inventor 杨洪雨付雁刘长玲佀庆波宋岩
Owner JILIN INST OF CHEM TECH
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