Method for producing avermectins by fermentation by using air-lift loop reactor

A loop reactor and air-lift technology, applied in the field of microbial fermentation, can solve the problems of high production cost, low utilization rate of fermenter equipment, energy waste, etc., to improve oxygen and nutrient transfer efficiency, reduce energy consumption and bacterial contamination. efficiency, reducing production costs

Inactive Publication Date: 2019-03-26
内蒙古新威远生物化工有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the fermentation equipment used for the fermentation and production of abamectin is a general-purpose stirring fermenter. The fermentation method is continuous fermentation, that is, all the base materials are put in at one time. The base materials are mainly high-concentration corn starch, soybean cake powder and yeast powder. , do not add materials in the middle until the tank is put into the tank. However, in order to pursue high output and low cost, too much starch, soybean cake powder and yeast powder are added in the basic material, resulting in a viscous material liquid, especially in the early stage of fermentation. Poor effect of dissolved oxygen in the culture medium
Therefore, it is necessary to increase the stirring speed and ventilation, and equip motors and stirring equipment

Method used

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  • Method for producing avermectins by fermentation by using air-lift loop reactor
  • Method for producing avermectins by fermentation by using air-lift loop reactor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Embodiment 1 adopts method fermentation of the present invention to produce Abamectin

[0055] Incline medium (g / L): glucose 25, agar powder 25, beef extract 10, potassium dihydrogen phosphate 1, the balance is water, adjust the pH to 7.0, sterilize at 121°C for 30 minutes, cool to room temperature for later use;

[0056] Seed medium (g / L): 25 corn starch, 25 soybean cake powder, 12 yeast powder, 12 peanut powder, 2.5 antifoaming agent, the balance is water, adjust the pH to 7.0, sterilize at 121°C for 30 minutes, cool to room temperature for later use ;

[0057] Fermentation medium (g / L): corn starch 110, soybean cake powder 25, yeast powder 18, ammonium sulfate 0.5, calcium carbonate 2.5, defoamer 3, the balance is water, adjust the pH to 7.0, sterilize at 121°C for 30 minutes, Cool to room temperature for later use;

[0058] Feed medium (g / L): cornstarch 300, defoamer 0.6, calcium carbonate 2.5, dissolved in appropriate amount of water, sterilized at 121°C for 30 m...

Embodiment 2

[0066] Embodiment 2 adopts method fermentation of the present invention to produce Abamectin

[0067]The difference between embodiment 2 and embodiment 1 is that the feeding time in the fermentation process is different, specifically:

[0068] Incline medium (g / L): glucose 25, agar powder 25, beef extract 10, potassium dihydrogen phosphate 1, the balance is water, adjust the pH to 7.0, sterilize at 121°C for 30 minutes, cool to room temperature for later use;

[0069] Seed medium (g / L): 25 corn starch, 25 soybean cake powder, 12 yeast powder, 12 peanut powder, 2.5 antifoaming agent, the balance is water, adjust the pH to 7.0, sterilize at 121°C for 30 minutes, cool to room temperature for later use ;

[0070] Fermentation medium (g / L): corn starch 110, soybean cake powder 25, yeast powder 18, ammonium sulfate 0.5, calcium carbonate 2.5, defoamer 3, the balance is water, adjust the pH to 7.0, sterilize at 121°C for 30 minutes, Cool to room temperature for later use;

[0071]...

Embodiment 3

[0079] Embodiment 3 adopts method fermentation of the present invention to produce Abamectin

[0080] The difference between embodiment 3 and embodiment 1 is that the feeding time in the fermentation process is different, specifically:

[0081] Incline medium (g / L): glucose 25, agar powder 25, beef extract 10, potassium dihydrogen phosphate 1, the balance is water, adjust the pH to 7.0, sterilize at 121°C for 30 minutes, cool to room temperature for later use;

[0082] Seed medium (g / L): 25 corn starch, 25 soybean cake powder, 12 yeast powder, 12 peanut powder, 2.5 antifoaming agent, the balance is water, adjust the pH to 7.0, sterilize at 121°C for 30 minutes, cool to room temperature for later use ;

[0083] Fermentation medium (g / L): corn starch 110, soybean cake powder 25, yeast powder 18, ammonium sulfate 0.5, calcium carbonate 2.5, defoamer 3, the balance is water, adjust the pH to 7.0, sterilize at 121°C for 30 minutes, Cool to room temperature for later use;

[0084...

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Abstract

The invention belongs to the technical field of microbial fermentation, and particularly relates to a method for producing avermectins by fed-batch fermentation by using an air-lift loop reactor. Themethod comprises the following steps that streptomyces avermitilis is inoculated to a slant culture medium for culture, and a spore slant is taken to prepare into a spore suspension; the spore suspension is inoculated to a seed culture medium and cultured to a logarithmic growth phase; and the streptomyces avermitilis spore suspension cultured to the logarithmic growth phase is transplanted into the air-lift loop reactor provided with a fermentation culture medium for fermentation culture, and a supplementary medium is added in batches in the middle and later periods of fermentation. The method adopts the air-lift loop reactor as a fermentation vessel, optimizes the fed-batch process and the composition of the supplementary medium, effectively reduces the bacterial infection rate in the fermentation culture process, and significantly increases the titer of the avermectins in fermentation liquid.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a method for producing abamectin by fed-batch fermentation in an airlift loop reactor. Background technique [0002] Avermectins are a group of 16-membered macrolide antibiotics with insecticidal, acaricidal and nematicidal activities, which are fermented by Streptomyces avermitilis in Streptomyces griseus. Natural abamectin contains 8 homologue components with similar structures, the main 4 species are A1a, A2a, B1a and B2a, and their total content is ≥80%; the corresponding 4 homologues with smaller proportions are A1b, A2b, B1b and B2b, the total content of which is ≤20%. Abamectin is widely used in agriculture and is a common pesticide variety. It is used as an insecticide to control Brassicaceae diamondback moth, cotton bollworm, mites, cabbage caterpillar, etc. [0003] At present, the fermentation equipment used for the fermentation and product...

Claims

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Application Information

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IPC IPC(8): C12P19/62C12N1/20C12R1/465
CPCC12N1/20C12P19/623
Inventor 郑智新高鹤永李军华张强刘文彬
Owner 内蒙古新威远生物化工有限公司
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