Application of flavonoid components to prepare pharmaceutical preparation for reducing enterogenous urotoxin or precursor molecules

A technology of pharmaceutical preparations and intestinal source urine, which is applied in the field of medicine, can solve the problems of low dialysis efficiency and filtration efficiency, inability to effectively remove protein-bound uremic toxins, and low clearance rate of protein-bound uremic toxins, and achieve reduced production , Significant kidney damage protection, reduce the effect of production

Inactive Publication Date: 2019-03-29
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the removal of uremic toxins mainly uses dialysis treatment to remove water-soluble small molecule uremic toxins in the blood. However, these methods cannot effectively remove protein-bound uremic toxins in the blood; The synthetic semipermeable membrane or the patient's own peritoneum used in peritoneal dialysis can only allow the free form of uremic toxin in the blood circulation to pass through, while the protein-bound uremic toxin has a small proportion of the free form, so the dialysis efficiency and filtration efficiency are both low. very low
Studies have shown that dialysis has a good effect on the removal of water-soluble small molecule urea toxins, but the removal rate of protein-bound urea toxins is very low

Method used

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  • Application of flavonoid components to prepare pharmaceutical preparation for reducing enterogenous urotoxin or precursor molecules
  • Application of flavonoid components to prepare pharmaceutical preparation for reducing enterogenous urotoxin or precursor molecules
  • Application of flavonoid components to prepare pharmaceutical preparation for reducing enterogenous urotoxin or precursor molecules

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Flavonoids reduce the experiment of model bacteria Escherichia coli to synthesize indole

[0020] 1. Experimental method

[0021] 1.1 Preparation of pharmaceutical compounds

[0022] Accurately weigh a certain amount of isoquercitrin (abbreviated Iso) standard substance, myricetin (abbreviated Myr) standard substance, quercetin (Hyp) standard substance, hyperoside (Que) standard substance, and add appropriate amount of DMSO to prepare Prepare the stock solution at the concentration required for the experiment and store it in a -20°C refrigerator for later use.

[0023] 1. Preparation of 2L-B medium

[0024] Accurately weigh 1 g of tryptone, 1 g of sodium chloride, and 0.5 g of yeast extract powder. Add 100ml of ultrapure water, stir to dissolve until clear and transparent without precipitation, sterilize it in a high-pressure steam sterilizer for 2 hours, and take it out of the refrigerator stored at 4°C for later use.

[0025] 1.3 Activation, dilution and...

Embodiment 2

[0037] Example 2 The experiment of flavonoids reducing the synthesis of indole by mixed bacteria in the cecum of rats

[0038] 1. Experimental method

[0039] With embodiment 1.

[0040] 2. Experimental results

[0041]Select four compounds, isoquercitrin, myricetin, hyperin, and quercetin, and give the compounds with a final concentration of 200 μM. After anaerobic culture with rat cecal bacteria for 12 and 24 hours, HPLC detects indole in the bacterial supernatant. content, and compared the inhibitory effects of the four compounds at a concentration of 200 μM on indole secretion.

[0042] The results showed that the four compounds of isoquercitrin, myricetin, hyperoside and quercetin could inhibit bacterial secretion of indole to varying degrees. Among the four compounds, isoquercitrin has the strongest inhibitory effect on indole secretion (eg figure 2 ). The four compounds co-cultured with the bacteria for 12 hours had no significant effect on the growth of the bacte...

Embodiment 3

[0043] Example 3 Flavonoids reduce the synthesis of indole by mixed bacteria in rat feces

[0044] 1. Experimental method

[0045] With embodiment 1.

[0046] 2. Experimental results

[0047] Four compounds, isoquercitrin, myricetin, hyperin, and quercetin were selected, given a final concentration of 200 μM, and after anaerobic culture with rat fecal bacteria for 12 hours, the indole content in the bacterial supernatant was detected by HPLC. The inhibitory effects of the four compounds on indole secretion at a concentration of 200 μM were compared.

[0048] The results showed that the four compounds could inhibit bacterial secretion of indole to varying degrees (such as image 3 shown).

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Abstract

The invention discloses an application of flavonoid components to prepare a pharmaceutical preparation for reducing enterogenous urotoxin or precursor molecules. The flavonoid components comprise flavonoid monomer components of isoquercitrin, hyperin, myricetin, gossypetin and the like. Through massive test screening, results prove that the flavonoid monomer components of the isoquercitrin, the hyperin, the myricetin, the gossypetin and the like can notably reduce the synthesis of the enterogenous urotoxin, can notably reduce the content of the enterogenous urotoxin, can develop an auxiliary pharmaceutical preparation for in vitro kidney replacement therapy of nephropathy dialysis, blood filtration and the like, and has good development and application prospects.

Description

technical field [0001] The invention belongs to the technical field of medicines, and in particular relates to the application of flavonoid components in the preparation of pharmaceutical preparations for reducing intestinal-derived uremic toxins and their precursor molecules. Background technique [0002] Chronic renal failure is the final result of the development of a variety of kidney diseases. It is a slow and irreversible pathological process due to damage to nephrons, decline in renal function, accumulation of metabolites and toxins, and disturbance of water, electrolyte and acid-base balance. Group of common clinical syndromes. Patients with renal failure cannot fully excrete metabolic waste in the body, thus accumulating toxic substances in the body that cause pathophysiological changes in the body and cause various symptoms and signs, called uremic toxins. It can induce complications such as bone disease, encephalopathy, cardiovascular disease, anemia, soft tissue...

Claims

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Application Information

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IPC IPC(8): A61K31/7048A61K31/352A61P13/12
CPCA61K31/352A61K31/7048A61P13/12
Inventor 郭建明李建萍张森江曙段金廒
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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