Method for extracting and purifying alkaloid in plants

A plant alkaloid and plant technology, applied in the field of alkaloid extraction and purification in plants, can solve the problems of cumbersome process flow, loss of effective components, etc., and achieve the effects of less extraction interference, saving intermediate processing links, and high efficiency

Inactive Publication Date: 2019-03-29
GUIZHOU INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although flash extraction combined with polymer flocculation can improve the purity of baicalein, the process of polymer flocculation is cumbersome, and some active ingredients will be lost during the precipitation process.

Method used

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  • Method for extracting and purifying alkaloid in plants
  • Method for extracting and purifying alkaloid in plants
  • Method for extracting and purifying alkaloid in plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Embodiment 1: Smelly goat alkaloid extraction

[0059] (1) Take 15g of stinky goat coarse powder that has been dried and crushed to 24 mesh at 40°C, add 450ml of ethanol solution with a volume fraction of 60% and soak for 1h, then use a flash extractor to extract at a voltage of 100V to obtain an extract , get filtrate after filtering, take rutone base as standard substance, detect its content with HPLC;

[0060] (2) Rotate the obtained filtrate to dryness to obtain the total extract, weigh 1g and dissolve it with 150ml 2% hydrochloric acid, filter, adjust the pH value of the filtrate to 8.5 with ammonia water, add 75ml of organic solvent ethyl acetate, and place it in a micro-extraction device Carry out micro-controlled extraction;

[0061] (3) After the extract was allowed to stand until the layers were completely separated, the organic layer was taken and evaporated to dryness to obtain the active ingredient total biological alkaloids. The content was detected by HP...

Embodiment 2

[0062] Embodiment 2: lotus leaf alkaloid extraction

[0063] (1) Take 12.5g of lotus leaf coarse powder that has been dried and crushed to 20 mesh at 45°C, add 250ml of ethanol solution with a volume fraction of 80% and soak for 1h, then use a flash extractor to extract at a voltage of 100V to obtain an extract , get the filtrate after filtering, take nuciferine as standard substance, measure its content with ultraviolet spectrophotometry;

[0064] (2) Rotate the obtained filtrate to dryness to obtain the total extract, weigh 1g and dissolve it with 150ml of 2% hydrochloric acid, filter, adjust the pH value of the filtrate to 10 with ammonia water, add 150ml of organic solvent dichloromethane, and place it in a micro-extraction device Carry out micro-controlled extraction;

[0065] (3) After the extract was allowed to stand until the layers were completely separated, the organic layer was taken and evaporated to dryness to obtain the active ingredient total biological alkaloi...

Embodiment 3

[0066] Embodiment 3: Uncaria alkaloid extraction

[0067] (1) Take 12.5 g of Uncaria coarse powder that has been dried and crushed to 24 mesh at 45°C, add 250 ml of ethanol solution with a volume fraction of 70% and soak for 1 hour, then use a flash extractor to extract at a voltage of 100 V to obtain an extract , get filtrate after filtering, take rhynchophylline as standard substance, measure its content with ultraviolet spectrophotometry;

[0068] (2) Rotate the obtained filtrate to dryness to obtain the total extract, weigh 1g and dissolve it with 150ml of 2% hydrochloric acid, filter, adjust the pH value of the filtrate to 8 with ammonia water, add 150ml of organic solvent dichloromethane, and place it in a micro-extraction device Carry out micro-controlled extraction;

[0069] (3) After the extract was allowed to stand until completely separated, the organic layer was taken and evaporated to dryness to obtain the active ingredient, total biological alkaloids. Taking rhy...

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Abstract

The invention belongs to the technical field of plant extraction, and particularly relates to a method for extracting and purifying alkaloid in plants and an application. The plant alkaloid is obtained through combination of flash extraction with micro-controlled extraction, and extremely short extraction time and a sufficient and complete distilling manner are used, so that the method disclosed by the invention concurrently has the characteristics of being quick and efficient; the extraction rate and the distilling rate of alkaloid are high, the content of obtained active components is high,the content of impurities is low, the operation time is short, the usage quantity of a solvent is low, and a convenient and efficient obtaining way is provided for extraction and purification of effective components in medical and edible plants.

Description

technical field [0001] The invention relates to the field of plant extraction and separation, in particular to a method and application for extracting and purifying alkaloids in plants. Background technique [0002] The chemical components contained in medicinal plants are very complex, including a variety of active components, some ineffective components, and toxic components; extracting the active components and further separating and purifying them to obtain effective monomers is the research goal of medicinal plants. an important item. At present, plant extraction and industrial production of its active ingredients have become emerging industries. How to improve the yield and purity has become an important measure to support the sustainable and stable development of this industry. [0003] At present, for the extraction of plant active ingredients, there are generally traditional solvent reflux methods, dipping methods, decoction methods, ultrasonic-assisted extraction ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/75A61K36/62A61K36/74A61K36/489
CPCA61K36/489A61K36/62A61K36/74A61K36/75A61K2236/51A61K2236/33A61K2300/00
Inventor 张振刁银娟杨海艳邱树毅禹志宏赵天明邹树良章绍凡
Owner GUIZHOU INST OF TECH
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