A microbubble preparation for ultrasonic diagnosis and sonodynamic therapy and its preparation method
An ultrasonic diagnosis and microbubble technology, applied in the field of biomedical materials, can solve problems such as increasing the pain of patients, and achieve the effects of reducing drug intake, less drug leakage, and enhancing efficiency
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Embodiment 1
[0038] A preparation method of the microbubble preparation is as follows figure 1 , As shown in 2, includes the following steps:
[0039] (1) Tiger Red Sodium Salt (0.5g, 4.92x 10 -4 mol), 8-bromooctanoic acid (0.33g, 1.48x 10 -3 mol) 5mL DMF was added and stirred at 80°C for 7 hours. After the DMF in the product was spin-dried under vacuum, 50mL of ether was added, stirred at room temperature 25°C for 18 hours, and filtered to obtain a red powder. Add 50mL of pure water to the red powder of the product, stir for 18 hours at room temperature 25 degrees Celsius, and filter to obtain product 1 0.44g, 3.95x10 -4 mol). Use mass spectrometry and nuclear magnetism to determine the relative molecular mass and structure of the product. 1 H NMR (500MHz, DMSO-d6): 0.80 (m, CH 2 ,2H),1.00(m,CH 2 ,2H),1.10(m,CH 2 ,4H),1.40(m,CH 2 ,2H),2.20(t,CH 2 COOH,2H),3.90(t,OCH 2 ,2H),7.50(s,ArH,2H),11.90(s,COOH,1H). Theoretical mass spectrum value: 1115.8, experimental value 1114.6. The reaction formul...
Embodiment 2
[0046] (1) Observe the microbubble preparations prepared in Example 1 under a fluorescence microscope to obtain imaging under white light and fluorescence images under 540nm green light excitation.
[0047] (2) Then take 1 mL of microbubbles for ultrasonic disintegration, perform dynamic light scattering and zeta potential evaluation, and then observe the nanoparticles under a transmission electron microscope to obtain image results.
[0048] The final result is image 3 As shown, the fluorescence image of the microbubble preparation illustrates the successful combination of rose bengal and the microbubbles, which are transformed into nanoparticles of appropriate size after ultrasonic disintegration.
Embodiment 3
[0050] The in vivo imaging effect evaluation of the microbubble preparation prepared in Example 1 mainly includes the following steps:
[0051] (1) Choose a 2-2.5kg female New Zealand white rabbit, and remove the abdominal hair. Urethane was injected via ear vein for anesthesia. The abdomen was coated with ultrasound coupling agent, and an ultrasound instrument was used for contrast-free renal radiography (frequency 3.5MHz, mechanical index 0.06 and 0.75). Save the image.
[0052] (2) Inject 1 mL of the microbubble preparation through the ear vein, and then inject 4 mL of normal saline to better enter the blood circulation. Then it was performed renal imaging (frequency of 5MHz, mechanical index of 0.06 and 0.75). Save the image.
[0053] The result is Figure 4 As shown, after the injection of rose bengal microbubbles, ultrasound contrast can be performed well under the condition of low mechanical index, which verifies its contrast function as microbubbles and proves that the pr...
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