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Extraction process of cordyceps polysaccharide

An extraction process and a technology for Cordyceps polysaccharide, applied in the field of extraction process of Cordyceps polysaccharide, can solve the problems of low extraction efficiency, low extraction yield, long production cycle, etc., and achieve broad market application potential, important economic value, and efficient extraction. Effect

Inactive Publication Date: 2019-04-05
FOSHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But these several methods have a common shortcoming that the production cycle is long; the traditional methods for extracting cordycepin in Cordyceps militaris, such as heat reflux extraction and Soxhlet extraction, are all extraction processes, which require a large amount of extraction solvent, and the extraction efficiency is low. Low
And all there is energy consumption big, and extraction yield is on the low side, and the shortcoming that production cost is higher

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1: the cultivation of Cordyceps bacterial classification

[0016] 1. Activation of strains

[0017] The Cordyceps fungus stored in the refrigerator was inoculated on a potato medium plate, put into an incubator at 25° C., and cultivated for three days to complete the activation of the strain.

[0018] 2. Expansion of cultivation

[0019] Pick the bacteria grown after activation and insert them into the potato liquid culture medium and put them into a shaker, and shake them for ten days under the conditions of 25°C and 150r / min.

[0020] 3. Fermentation culture

[0021] Configure the medium with the following proportions: glucose 10g / L, sucrose 25g / L, corn flour 10g / L, maltose 5g / L, peptone 4g / L, ammonium nitrate 3g / L, magnesium sulfate 0.8g / L, dipotassium hydrogen phosphate 0.4g / L, potassium dihydrogen phosphate 0.4g / L, ferrous sulfate 1.4g / L, vitamin B1 0.03g / L, glycine 0.8g / L, yeast powder 4g / L and soybean meal 4g / L, after sterilization , take the expan...

Embodiment 2

[0022] Embodiment 2: polysaccharide extraction

[0023] The strains fermented and cultivated in Example 1 were subjected to suction filtration treatment, the mycelia obtained by suction filtration contained intracellular crude polysaccharides, and the mycelium obtained by suction filtration was put into a blast drying oven to dry for 6-7 hours, At this time, the mycelium is completely dry, and its mass is weighed and recorded; after weighing, the mycelium is cut into pieces and put into a mortar, poured into liquid nitrogen for grinding, and the mycelium is ground to a fineness of about 60 mesh That’s it; pour the crushed powder into a jar, add 15ml of pure water, and put it in an ultrasonic wave for ultrasonic extraction for 30 minutes; after the extraction, centrifuge at 4000 rpm for 15 minutes to obtain the supernatant solution, and the supernatant was stored in a test tube.

Embodiment 3

[0024] Embodiment 3: the purification of polysaccharide

[0025] Place the supernatant obtained in Example 2 in a jar, add 2 times the volume of the supernatant with 80% ethanol, precipitate for 24 hours, filter to obtain the intracellular crude solid polysaccharide, wash the solid 2-3 times with 20 ml of ether, Filtrate to obtain the intracellular crude solid polysaccharide without fat and protein, and store it in the refrigerator for later use.

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PUM

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Abstract

The invention provides an extraction process of cordyceps polysaccharide, comprising extraction of the polysaccharide and purification of the polysaccharide. The extraction process herein enables cordyceps polysaccharide to be effectively extracted; the cost is lowered; the extraction process has important economic valve and great market application potential and is suitable for industrial production.

Description

technical field [0001] The invention belongs to the field of biological products, and in particular relates to an extraction process of cordyceps polysaccharide. Background technique [0002] Cordyceps polysaccharide is a kind of polymer compound, it is one of the main active components of Cordyceps sinensis, and it is the pharmacological active substance with the highest content in Cordyceps sinensis. A large number of pharmacological experiments have shown that Cordyceps polysaccharides have anti-tumor, immune-regulating, hypoglycemic, hypolipidemic, anti-atherosclerosis, kidney-protecting, analgesic and anti-radiation effects. [0003] Traditional methods for extracting Cordyceps polysaccharides from Cordyceps sinensis, such as decoction, reflux, continuous reflux, dipping, percolation, etc., have been well known and detailed. But these several methods have a common shortcoming that the production cycle is long; the traditional methods for extracting cordycepin in Cordyc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 刘燊邓赣奇冷桂华张辉华林树茂
Owner FOSHAN UNIVERSITY
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