Novel long-acting degradable biological nano membrane slowing releasing VEGF and bFGF and preparation method of nano membrane

A bio-nano-film and nano-fiber film technology, applied in the field of nano-biomedical materials, can solve the problems of poor controlled release time and controlled release concentration, achieve good biocompatibility, improve treatment effect, and reduce the risk of recurrence. Effect

Inactive Publication Date: 2019-04-12
SHANGHAI TONGJI HOSPITAL
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] In the existing reports, the Chinese patent "A Preparation Method for Biodegradable Periodontal Tissue Regeneration Film" (Chinese Patent No.: CN201010105573.2, Authorized Announcement No.: CN101791431A) discloses that by electrospinning, A nanostructured biodegradable material film (such as polylactic acid) was prepared, and the growth factors that in

Method used

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  • Novel long-acting degradable biological nano membrane slowing releasing VEGF and bFGF and preparation method of nano membrane
  • Novel long-acting degradable biological nano membrane slowing releasing VEGF and bFGF and preparation method of nano membrane
  • Novel long-acting degradable biological nano membrane slowing releasing VEGF and bFGF and preparation method of nano membrane

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Example Embodiment

[0029] Example 1

[0030] Polylactic acid was dissolved in chloroform / ethanol mixed solution to prepare a solution with a concentration of 10% (wt). Add this mixture solution to the electrospinning syringe controlled by the syringe pump, set the voltage of the high-voltage generator to 20KV, and the collection distance to 15cm, and a membrane composed of ultrafine fibers with a diameter of nanometer scale can be obtained, and then the The membrane was washed several times with deionized water and dried in vacuum before use. Step 1: Soak the dried membrane in polyethyleneimine (0.5-2mg / ml) solution for 30-60 minutes, and then soak it in deionized water for 10 minutes. Then VEGF and bFGF are made into a mixed growth factor solution with a concentration of 0.1-10 μg / ml, the membrane is soaked in the growth factor solution for 10-20 minutes for adsorption, and then washed with deionized water. Step 2: Then immerse in hyaluronic acid (0.5-2mg / ml) solution, growth factor solution ...

Example Embodiment

[0031] Example 2

[0032] Polylactic acid was dissolved in chloroform / ethanol mixed solution to prepare a solution with a concentration of 8% (wt). Add this mixture solution to the electrospinning syringe controlled by the syringe pump, set the voltage of the high-voltage generator to 15KV, and the collection distance to 15cm, and a membrane composed of ultrafine fibers with a diameter of nanometer scale can be obtained, and then the The membrane was washed several times with deionized water and dried in vacuum before use. Step 1: Soak the dried membrane in polyethyleneimine (0.5-2mg / ml) solution for 30-60 minutes, and then soak it in deionized water for 10 minutes. Then VEGF and bFGF are made into a mixed growth factor solution with a concentration of 0.1-10 μg / ml, the membrane is soaked in the growth factor solution for 10-20 minutes for adsorption, and then washed with deionized water. Step 2: Then immerse in hyaluronic acid (0.5-2mg / ml) solution, growth factor solution a...

Example Embodiment

[0033] Example 3

[0034] Polylactic acid was dissolved in a chloroform / ethanol mixed solution to prepare a solution with a concentration of 9% (wt). Add this mixture solution to the electrospinning syringe controlled by the syringe pump, set the voltage of the high-voltage generator to 20KV, and the collection distance to 15cm, and a membrane composed of ultrafine fibers with a diameter of nanometer scale can be obtained, and then the The membrane was washed several times with deionized water and dried in vacuum before use. Step 1: Soak the dried membrane in polyethyleneimine (0.5-2mg / ml) solution for 30-60 minutes, and then soak it in deionized water for 10 minutes. Then VEGF and bFGF are made into a mixed growth factor solution with a concentration of 0.1-10 μg / ml, the membrane is soaked in the growth factor solution for 10-20 minutes for adsorption, and then washed with deionized water. Step 2: Then immerse in hyaluronic acid (0.5-2mg / ml) solution, growth factor solution...

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Abstract

The invention relates to the technical field of nano biomedical materials, and particularly discloses a novel long-acting degradable biological nano membrane slowing releasing VEGF and bFGF and a preparation method of the nano membrane. The nanofiber membrane obtained by the method of the invention has the following characteristics: 1, The nano membrane is non-toxic to human tissues, does not produce harmful substances after degradation in the body, and has good biocompatibility; 2, the nano membrane can avoid the inactivation of growth factors by organic solvents, strong acids and alkalis, high temperature and other factors, ensures that the growth factors loaded on the membrane maintain good activity and has a best therapeutic effect; and 3, the nano membrane avoids trauma caused by direct injection of growth factors into a ventricle and the problem of uncontrollable concentration, has a good effect of promoting vascular and nerve regeneration, can be applied to insufficient vascularregeneration after indirect vascular bypass surgery in neurosurgery, improves the therapeutic effect of related ischemic cerebrovascular diseases, and reduces the risk of recurrence of ischemic stroke.

Description

technical field [0001] The invention relates to the technical field of nano biomedical materials, in particular to a novel long-acting slow-release VEGF and bFGF degradable bio-nano membrane and a preparation method thereof. Background technique [0002] In recent years, porous electrospun films have been used as composite materials in optics, electrical appliances, and pharmacy because their diameter is similar to that of the body's extracellular matrix, and they have large specific surface area, adjustable diameter and pore size, and three-dimensional network structure. Show great application potential. Nanomaterials are prepared by electrospinning technology. In a high-voltage electric field, the degradable polymer solution or melt is stretched (such as polycaprolactone, polylactose, polylactic acid-glycolic acid copolymer, collagen, silk fibroin) Nanofiber film), droplets are ejected from the tip of the spinneret to form a jet, and under the interaction of electrostatic...

Claims

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Application Information

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IPC IPC(8): A61L27/34A61L27/18A61L27/50A61L27/54A61L27/58
CPCA61L27/18A61L27/34A61L27/50A61L27/54A61L27/58A61L2300/252A61L2300/414A61L2300/602A61L2400/12A61L2400/18A61L2420/02A61L2420/06A61L2420/08A61L2430/32C08L89/00C08L67/04
Inventor 海舰王大鹏吴一芳苏少华
Owner SHANGHAI TONGJI HOSPITAL
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