A kind of methanogen and its application
A technology of methane bacteria and methane, applied in the field of methane bacteria, can solve the problems of meat and milk quality and human health hazards, and achieve the effect of reducing toxicity
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Embodiment 1
[0018] Example 1 Screening bacterial strain LGM-RCC1
[0019] 1. Materials and methods
[0020] 1.1 Inoculum
[0021] Goat fresh rumen juice.
[0022] 1.2 Medium composition and preparation
[0023] 1.2.1 Medium composition
[0024] Methanogen BRN medium, the composition is shown in Table 1. The tests of the present invention are all carried out using BRN medium.
[0025] Table 1 Methanogen BRN medium composition (1L formula)
[0026]
[0027] Note: The solvent of the above solutions is water.
[0028] 1.2.2 Cell-free rumen fluid
[0029] Fresh goat rumen fluid was collected through a rumen fistula, and then filtered through two layers of gauze. The liquid was centrifuged at 12,000 rpm at 4°C for 20 minutes, and the supernatant was taken, and stored at -20°C for later use.
[0030] 1.2.3 Volatile fatty acid solution
[0031] Accurately measure acetic acid, 6.85mL; propionic acid, 3.0mL; butyric acid, 1.84mL; 2-methylbutyric acid, 0.55mL; isobutyric acid, 0.47mL; I...
Embodiment 2
[0044] Example 2 LGM-RCC1 utilizes different substrates to produce methane
[0045] The components and preparation method of the BRN medium are the same as in Example 1, except that the substrates of the fermentation medium are respectively methanol (MeOH, 0.06mol / L), monomethylamine (MMA, 0.06mol / L), dimethylamine (DMA, 0.03mol / L), trimethylamine (TMA, 0.02mol / L) or methyl 3-methylthiopropionate (M 3 PT, 0.06mol / L). BRN medium was used to prepare the inoculum of strain LGM-RCC1, and the substrate was a mixture of five methyl compounds (0.01mol / L for each substrate), and cultured at 39°C for 20 days to inoculate. Afterwards, the strains were inserted into a fermentation tube of 10 mL of BRN medium that had been preheated to 39°C and contained penicillin, vitamin solution, and substrate solution, and then 100Kpa of hydrogen gas was fed into the fermentation tube, cultured at 39°C, and methane was measured. output, see figure 1 .
[0046] Such as figure 1 As shown, LGM-RCC1...
Embodiment 3
[0047] The influence of embodiment 3pH on LGM-RCC1 methanogenic
[0048] The components and preparation method of the BRN medium, the inoculum preparation method (medium pH 6.9-7.0), and the fermentation are the same as in Example 2, except that the substrate is only trimethylamine, and the concentration is 0.02mol / L. The pH of the culture medium in the fermentation stage is set to 5.0, 5.5, 6.0, 6.5, 7.0, 7.5, 8.0 respectively, the substrate is trimethylamine, the bacteria are inserted into the preheated to 39°C, and contain penicillin, streptomycin, vitamin solution , trimethylamine (0.02mol / L) solution in the fermentation tube of 10mL BRN medium, the fermentation tube was fed with 100Kpa hydrogen, cultivated at 39°C and measured the methane production, the results are shown in figure 2 , LGM-RCC1 can grow between pH 6.5-8.0.
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