Method for improving efficiency of killing retained bacteria by aminoglycoside antibiotics through low ion shock

An aminoglycoside and antibiotic technology, applied in botanical equipment and methods, chemicals for biological control, biocides, etc., can solve problems such as long contact time and bacterial resistance, reduce side effects, eliminate Bacterial persistence, the effect of reducing bacterial tolerance

Active Publication Date: 2019-04-16
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] Treating bacterial infections with antibiotics is a typical clinical and aquaculture treatment, but with the widespread use of antibiotics, some bacteria have developed drug resistance
In addition, in some chronic persisten

Method used

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  • Method for improving efficiency of killing retained bacteria by aminoglycoside antibiotics through low ion shock
  • Method for improving efficiency of killing retained bacteria by aminoglycoside antibiotics through low ion shock
  • Method for improving efficiency of killing retained bacteria by aminoglycoside antibiotics through low ion shock

Examples

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Example Embodiment

[0023] Example 1

[0024] Low ion shock enhances the efficiency of aminoglycoside antibiotics in killing type I Escherichia coli

[0025] 1. Activate the standard strain of Escherichia coli (E.coli BW25113). Draw 1μl of the bacterial solution stored in the refrigerator at -80℃ with 40% glycerol, and inoculate it in 1ml M9 plus glucose liquid medium (configuration ingredients: M9 medium, 5g / L glucose, 1.5g / L (NH 4 ) 2 SO 4 , 1mg / L VB 1 , PH=7.3), 37℃ shaker (220rpm) culture to plateau, re-diluted 1000 times and then inoculated in 10ml M9 plus glucose liquid medium, 37℃ shaker (220rpm) culture to logarithmic phase (OD 600 =0.5-0.6). Take 2 5ml EP tubes, each tube with 4ml bacteria liquid centrifugation (5000g, 4℃, 5min), remove the supernatant; resuspend in the same volume of 4℃ pre-cooled M9 liquid medium and centrifuge (5000g, 4℃, 5min) ), remove the supernatant; use 1ml of normal temperature M9 liquid medium (PH=7.3) to resuspend and centrifuge (5000g, 4℃, 5min), remove the super...

Example Embodiment

[0036] Example 2

[0037] Low ion shock enhances the efficiency of aminoglycoside antibiotics in killing type Ⅱ Escherichia coli

[0038] 1. Activate the standard strain of Escherichia coli (E.coli BW25113). Aspirate 1μl of bacteria liquid stored in a refrigerator at -80℃ with 40% glycerol, inoculate it in 1ml MHB liquid medium (Mueller-Hinton broth), and incubate for 24h on a shaker (220rpm) at 35℃ (platform period 10 9 CFU / ml), re-diluted 1000 times and then inoculated in 2ml MHB liquid medium, cultivated on a shaker (220rpm) at 35℃ for 24h (platform period 10 9 CFU / ml). Take 1 1.5ml EP tube, suck 1ml bacterial liquid and centrifuge (13000g, 2min), remove the supernatant; resuspend with 1ml M9 carbon-free liquid medium and transfer 9ml M9 carbon-free liquid medium into sterile shake flasks (I.e., the bacterial solution is 10 8 The concentration of CFU / ml is continued to be cultured in a medium with M9 carbon-free liquid medium as a substrate), and treated in a shaker (220 rpm) a...

Example Embodiment

[0049] Example 3

[0050] Low ion shock enhances the efficiency of aminoglycoside antibiotics in killing type Ⅲ Staphylococcus aureus

[0051] 1. Activated standard strain of Staphylococcus aureus (S. aureus ATC25923). Aspirate 1μl of bacteria liquid stored in a refrigerator at -80℃ with 40% glycerol, inoculate it in 1ml MHB liquid medium (Mueller-Hinton broth), and incubate for 24h on a shaker (220rpm) at 35℃ (platform period 10 9 CFU / ml), re-diluted 1000 times and then inoculated in 2ml MHB liquid medium, cultivated on a shaker (220rpm) at 35℃ for 24h (platform period 10 9 CFU / ml). Take 1 1.5ml EP tube, pipette 1ml bacterial liquid and centrifuge (13000g, 2min), remove the supernatant; resuspend with 1ml YNB non-carbon source liquid medium (Yeast nitrogen base broth, without anamino acid) and 9ml YNB non-carbon source liquid The culture medium was transferred into sterile shake flasks (ie: the bacterial solution was 10 8 The concentration of CFU / ml is continued to be cultured in...

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Abstract

The invention discloses a method for improving the efficiency of killing retained bacteria by aminoglycoside antibiotics through low ion shock. The method comprises the following steps of: preparing antibiotics into an antibiotic pretreatment solution with a certain concentration by adopting an isotonic solution or a sterile aqueous solution, wherein the isotonic solution is a 0.3M glycerol solution or a 0.9% Nacl solution, and the sterile aqueous solution is double distilled water. The antibiotic pretreatment solution is used for carrying out resuspension treatment on bacterial liquid containing retained bacteria to be killed. The method can greatly improve the efficiency of killing the remained bacteria by the aminoglycoside antibiotics, effectively eliminate the retention of bacteria, reduce the tolerance of the bacteria, and reduce the dosage and the administration time on the premise of achieving the same treatment effect, thereby reducing the side effect of the antibiotics.

Description

technical field [0001] The invention belongs to the field of antibiotics and microorganisms, and in particular relates to a method for improving the efficiency of aminoglycoside antibiotics in killing persistent bacteria by low ion shock. Background technique [0002] Treating bacterial infections with antibiotics is a typical clinical and aquaculture treatment method, but with the widespread use of antibiotics, some bacteria have developed drug resistance. In addition, in some chronic persistent infections and biofilm infections, the bacteria did not undergo drug-resistant mutations, but required longer exposure time when using antibiotics, which is related to the persistence of bacteria. [0003] Persisters are a small portion of bacteria with phenotype drug resistance in the bacterial population. There is no drug resistance mutation in their own genes, but they are insensitive and resistant to antibiotics due to their low metabolism, no growth or slow growth. [0004] Nu...

Claims

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Application Information

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IPC IPC(8): A61L2/02A61L2/18A01N43/16A01N47/44A01P1/00
CPCA01N43/16A01N47/44A61L2/02A61L2/18
Inventor 付新苗陈钟毓高媛媛
Owner FUJIAN NORMAL UNIV
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